The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "Ajar" intermediate conformation in the nucleotide selection mechanism

Journal of Biological Chemistry

Volumn 286, Issue 22, 2011, Pages 19758-19767

  Wu, Eugene Y ^a,b   Beese, Lorena S ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b UNIVERSITY OF RICHMOND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; CRYSTALLOGRAPHIC ANALYSIS; DNA POLYMERASE; DNA SYNTHESISS; HIGH FIDELITY; INSERTION SITE; INTERMEDIATE CONFORMATION; PRIMER TERMINUS; REACTION PATHWAYS; SELECTION MECHANISM; TERNARY COMPLEX; TRIPHOSPHATE; WILD TYPES;

AMINO ACIDS; CRYSTAL STRUCTURE; DNA; NUCLEOTIDES; REACTION INTERMEDIATES; REACTION KINETICS; SYNTHESIS (CHEMICAL);

POLYMERS;

DNA DIRECTED DNA POLYMERASE BETA; NUCLEOSIDE TRIPHOSPHATE; NUCLEOTIDE; TYROSINE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME STRUCTURE; GEOBACILLUS STEAROTHERMOPHILUS; MUTANT; NONHUMAN; PRIORITY JOURNAL; WILD TYPE;

BACTERIAL PROTEINS; DNA POLYMERASE I; DNA, BACTERIAL; GEOBACILLUS STEAROTHERMOPHILUS; KINETICS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY;

EID: 79957603036     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.191130     Document Type: Article

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