A simple and high-yield method for preparation of rat microglial cultures utilizing Aclar plastic film

Neuropathology

Volumn 31, Issue 3, 2011, Pages 215-222

  Seki, Yoshihiro ^a,b   Suzuki, Satoshi O ^a   Masui, Kenta ^a   Harada, Shiori ^a,b   Nakamura, Seiji ^b   Kanba, Shigenobu ^a   Iwaki, Toru ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Aclar plastic film; Isolation method; Microglia; Primary culture; SV40 T antigen

Indexed keywords

CALCIUM PHOSPHATE; CD11B ANTIGEN; CD45 ANTIGEN; CD68 ANTIGEN; CELL MARKER; EXCITATORY AMINO ACID TRANSPORTER; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; VIRUS T ANTIGEN;

ANIMAL TISSUE; ARTICLE; ASTROCYTE; CELL CLONE; CELL IMMORTALIZATION; CELL ISOLATION; CELL LINEAGE; CELL PROLIFERATION; CENTRAL NERVOUS SYSTEM DISEASE; CONTROLLED STUDY; FILM; GENETIC CODE; GENETIC TRANSFECTION; MICROGLIA; NERVE CELL CULTURE; NERVE DEGENERATION; NEUROPROTECTION; NONHUMAN; PHAGOCYTOSIS; PRECIPITATION; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; SIMIAN VIRUS 40;

ANIMALS; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CELL SEPARATION; CELL TRANSPLANTATION; FEMALE; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; MICROGLIA; MICROSPHERES; PLASTICS; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS;

EID: 79957536532     PISSN: 09196544     EISSN: 14401789     Source Type: Journal    
DOI: 10.1111/j.1440-1789.2010.01163.x     Document Type: Article

References (31)

1. Tambuyzer BR, Ponsaerts P, Nouwen EJ. Microglia: gatekeepers of central nervous system immunology. J Leukoc Biol 2009; 85: 352-370.
2. Aloisi F. Immune function of microglia. Glia 2001; 36: 165-179.
3. Ford AL, Goodsall AL, Hickey WF, Sedgwick JD. Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+ T cells compared. J Immunol 1995; 154: 4309-4321.
4. Hao C, Richardson A, Fedoroff S. Macrophage-like cells originate from neuroepithelium in culture: characterization and properties of the macrophage-like cells. Int J Dev Neurosci 1991; 9: 1-14.
5. Ganter S, Northoff H, Mannel D, Gebicke-Harter PJ. Growth control of cultured microglia. J Neurosci Res 1992; 33: 218-230.
6. Colton CA, Yao J, Taffs RE, Keri JE, Oster-Granite ML. Abnormal production of interleukin-1 by microglia from trisomy 16 mice. Neurosci Lett 1991; 132: 270-274.
7. Saura J, Tusell JM, Serratosa J. High-yield isolation of murine microglia by mild trypsinization. Glia 2003; 44: 183-189.
8. Giulian D, Baker TJ. Characterization of amoeboid microglia isolated from developing mammalian brain. J Neurosci 1986; 6: 2163-2178.
9. Frei K, Bordmer S, Schwerdel C, Fontana A. Astrocyte-derived interleukin 3 as a growth factor for microglia cells and peritoneal macrophages. J Immunol 1986; 137: 3521-3527.
10. Imai F, Suzuki H, Oda J etal. Neuroprotective effect of exogenous microglia in global brain ischemia. J Cereb Blood Flow Metab 2007; 27: 488-500.
11. Sawada M, Suzumura A, Yamamoto H, Marunouchi T. Activation and proliferation of the isolated microglia by colony stimulating factor-1 and possible involvement of protein kinase C. Brain Res 1990; 509: 119-124.
12. Electron Microscopy Sciences. [homepage on the Internet]. Chicago: American Medical Association [updated 23 Aug 2001; cited 12 Aug 2002.] AMA office of Group practice Liaison. Available from URL: .
13. Kim DW, Uetsuki T, Kaziro Y, Yamaguchi N, Sugano S. Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene 1990; 91: 217-223.
14. Kakita A, Goldman JE. Patterns and dynamics of SVZ cell migration in the postnatal forebrain: monitoring living progenitors in slice preparations. Neuron 1999; 23: 461-472.
15. Ponomarev ED, Novikova M, Maresz K, Shriver LP, Dittel BN. Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J Immunol Methods 2005; 300: 32-46.
16. Gingras M, Gagnon V, Minotti S, Durham HD, Berthod F. Optimised protocols for isolation of primary motor neurons, astrocytes and microglia from embryonic mouse spinal cord. J Neurosci Methods 2007; 163: 111-118.
17. Moussaud S, Draheim HJ. A new method to isolate microglia from adult mice and culture them for an extended period of time. J Neurosci Methods 2010; 187: 243-253.
18. Sawada M, Imai F, Suzuki H, Hayakawa M, Kanno T, Nagatsu T. Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain. FEBS Lett 1998; 433: 37-40.
19. van Furth R. Current view on the mononuclear phagocyte system. Immunobiology 1982; 161: 178-185.
20. Williams K, Bar-Or A, Ulvestad E, Olivier A, Antel JP, Yong VW. Biology of adult human microglia in culture: comparisons with peripheral blood monocytes and astrocytes. J Neuropathol Exp Neurol 1992; 51: 538-549.
21. McGeer PL, Kawamata T, Walker DG, Akiyama H, Tooyama I, McGeer EG. Microglia in degenerative neurological disease. Glia 1993; 7: 84-92.
22. Neumann H. Control of glial immune function by neurons. Glia 2001; 36: 191-199.
23. Pirami L, Stockinger B, Corradin SB etal. Mouse macrophage clones immortalized by retroviruses are functionally heterogeneous. Proc Natl Acad Sci U S A 1991; 88: 7543-7547.
24. Suzumura A, Sawada M, Takayanagi T. Production of interleukin-12 and expression of its receptors by murine microglia. Brain Res 1998; 787: 139-142.
25. Salimi K, Moser K, Zassler B etal. Glial cell line-derived neurotrophic factor enhances survival of GM-CSF dependent rat GMIRI-microglial cells. Neurosci Res 2002; 43: 219-221.
26. Okada M, Irie S, Sawada M etal. Pepstatin A induces extracellular acidification distinct from aspartic protease inhibition in microglial cell lines. Glia 2003; 43: 167-174.
27. Hassan NF, Rifat S, Campbell DE, McCawley LJ, Douglas SD. Isolation and flow cytometric characterization of newborn mouse brain-derived microglia maintained in vitro. J Leukoc Biol 1991; 50: 86-92.
28. Abromson-Leeman S, Hayashi M, Martin C etal. T cell responses to myelin basic protein in experimental autoimmune encephalomyelitis-resistant BALB/c mice. J Neuroimmunol 1993; 45: 89-101.
29. Fischer HG, Nitzgen B, Germann T, Degitz K, Daubener W, Hadding U. Differentiation driven by granulocyte-macrophage colony-stimulating factor endows microglia with interferon-gamma-independent antigen presentation function. J Neuroimmunol 1993; 42: 87-95.
30. Slepko N, Levi G. Progressive activation of adult microglial cells in vitro. Glia 1996; 16: 241-246.
31. Pinteaux E, Parker LC, Rothwell NJ, Luheshi GN. Expression of interleukin-1 receptors and their role in interleukin-1 actions in murine microglial cells. J Neurochem 2002; 83: 754-763.