Targeting the tcdA gene: Is this appropriate for detection of A and/or B Clostridium difficile toxin-producing strains?

Journal of Clinical Microbiology

Volumn 49, Issue 6, 2011, Pages 2383-

  Kozak, Ken ^a   Elagin, Vecheslav ^a  

^a Meridian Bioscience   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAY; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; CULTURE TECHNIQUE; GENE; GENE DELETION; GENE TARGETING; INTERMETHOD COMPARISON; LETTER; LOOP MEDIATED ISOTHERMAL DNA AMPLIFICATION; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TCDA GENE; TCDB GENE;

BACTERIAL PROTEINS; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CONSERVED SEQUENCE; DNA PRIMERS; ENTEROTOXINS; MOLECULAR DIAGNOSTIC TECHNIQUES; SENSITIVITY AND SPECIFICITY; VIRULENCE FACTORS;

CLOSTRIDIUM DIFFICILE;

EID: 79957474450     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00523-11     Document Type: Letter

References (5)

1. Couturier, B., and R. She. 2010. The illumigene C. difficile assay detects both A+B+ and A-B+ toxin-producing strains of Clostridium difficile, abstr. ID 55. Abstr. Mol. Pathol. 16th Annu. Meet., San Jose, CA.
2. Coyle, K., S. Elagin, J. Kraft, and X. K. Deng. 2010. Reactivity of Clostridium difficile toxinotypes with the illumigene C. difficile molecular assay, abstr. C-1107, p. 219. Abstr. 110th Gen. Meet. Am. Soc. Microbiol., San Diego, CA.
3. Norén, T., I. Alriksson, J. Andersson, T. Åkerlund, and M. Unemo. 2011. Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard. J. Clin. Microbiol. 49:710-711.
4. Rupnik, M. 2008. Heterogeneity of large clostridial toxins: importance of Clostridium difficile toxinotypes. FEMS Microbiol. Rev. 32:541-555.
5. Rupnik, M., V. Avesani, M. Janc, C. Eichel-Streiber, and M. Delmee. 1998. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36:2240-2247. (Pubitemid 28324074)