Real-time PCR improves helicobacter pylori detection in patients with peptic ulcer bleeding

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Ramírez Lázaro, María José ^a,b   Lario, Sergio ^a,b   Casalots, Alex ^c   Sanfeliu, Esther ^c   Boix, Loreto ^b,d   García Iglesias, Pilar ^a   Sánchez Delgado, Jordi ^a,b   Montserrat, Antònia ^a,b   Bella Cueto, Maria Rosa ^c   Gallach, Marta ^a   Sanfeliu, Isabel ^a,e   Segura, Ferran ^a,e   Calvet, Xavier ^a,b  

^a PARC TAULÍ HOSPITAL UNIVERSITARI   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c HOSPITAL DE SABADELL   (Spain)

^d HOSPITAL CLÍNIC   (Spain)

^e Spanish Network for the Research in Infectious Diseases ^*  (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; RNA 16S; RNA 23S; UNCLASSIFIED DRUG; UREA PROTEIN; PRIMER DNA;

ADULT; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; FEMALE; HELICOBACTER INFECTION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MALE; MOLECULAR DIAGNOSIS; NONHUMAN; PEPTIC ULCER BLEEDING; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS; AGED; BACTERIAL GENE; BIOPSY; BLEEDING; GENETICS; HELICOBACTER PYLORI; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; NUCLEOTIDE SEQUENCE; PATHOLOGY; PEPTIC ULCER; POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE;

HELICOBACTER PYLORI;

ADULT; AGED; BASE SEQUENCE; BIOPSY; DNA PRIMERS; GENES, BACTERIAL; HELICOBACTER PYLORI; HEMORRHAGE; HUMANS; IMMUNOHISTOCHEMISTRY; MIDDLE AGED; PEPTIC ULCER; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIES SPECIFICITY;

EID: 79956369208     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020009     Document Type: Article

References (45)

1. Suerbaum S, Michetti P, (2002) Helicobacter pylori infection. N Engl J Med 347: 1175-1186.
2. Gisbert JP, Khorrami S, Carballo F, Calvet X, Gene E, et al. (2004) Meta-analysis: Helicobacter pylori eradication therapy vs. antisecretory non-eradication therapy for the prevention of recurrent bleeding from peptic ulcer. Aliment Pharmacol Ther 19: 617-629.
3. Gisbert JP, Pajares JM, (2003) Helicobacter pylori and bleeding peptic ulcer: what is the prevalence of the infection in patients with this complication? Scand J Gastroenterol 38: 2-9.
4. Gisbert JP, Abraira V, (2006) Accuracy of Helicobacter pylori diagnostic tests in patients with bleeding peptic ulcer: a systematic review and meta-analysis. Am J Gastroenterol 101: 848-863.
5. Raderer M, Streubel B, Wohrer S, Hafner M, Chott A, (2006) Successful antibiotic treatment of Helicobacter pylori negative gastric mucosa associated lymphoid tissue lymphomas. Gut 55: 616-618.
6. Bik EM, Eckburg PB, Gill SR, Nelson KE, Purdom EA, et al. (2006) Molecular analysis of the bacterial microbiota in the human stomach. Proc Natl Acad Sci USA 103: 732-737.
7. He Q, Wang JP, Osato M, Lachman LB, (2002) Real-time quantitative PCR for detection of Helicobacter pylori. J Clin Microbiol 40: 3720-3728.
8. Tankovic J, Chaumette-Planckaert MT, Deforges L, Launay N, Le Glaunec JM, et al. (2007) Routine use of real-time PCR for detection of Helicobacter pylori and of clarithromycin resistance mutations. Gastroenterol Clin Biol 31: 792-795.
9. Lo CC, Lai KH, Peng NJ, Lo GH, Tseng HH, et al. (2005) Polymerase chain reaction: a sensitive method for detecting Helicobacter pylori infection in bleeding peptic ulcers. World J Gastroenterol 11: 3909-3914.
10. Ciesielska U, Jagoda E, Marciniak Z, (2002) Value of PCR technique in detection of Helicobacter pylori in paraffin-embedded material. Folia Histochem Cytobiol 40: 129-130.
11. Scholte GH, van Doorn LJ, Quint WG, Lindeman J, (1997) Polymerase chain reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, paraffin-embedded gastric biopsies. Diagn Mol Pathol 6: 238-243.
12. Weiss J, Mecca J, da Silva E, Gassner D, (1994) Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients. J Clin Microbiol 32: 1663-1668.
13. Kobayashi D, Eishi Y, Ohkusa T, Ishige, Suzuki T, et al. (2002) Gastric mucosal density of Helicobacter pylori estimated by real-time PCR compared with results of urea breath test and histological grading. J Med Microbiol 51: 305-311.
14. Schabereiter-Gurtner C, Hirschl AM, Dragosics B, Hufnagl P, Puz S, et al. (2004) Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol 42: 4512-4518.
15. Lascols C, Lamarque D, Costa JM, Copie-Bergman C, Le Glaunec JM, et al. (2003) Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by real-time PCR. J Clin Microbiol 41: 4573-4577.
16. Guell M, Artigau E, Esteve V, Sanchez-Delgado J, Junquera F, et al. (2006) Usefulness of a delayed test for the diagnosis of Helicobacter pylori infection in bleeding peptic ulcer. Aliment Pharmacol Ther 23: 53-59.
17. Castro-Fernandez M, Sanchez-Munoz D, Garcia-Diaz E, Miralles-Sanchiz J, Vargas-Romero J, (2004) Diagnosis of Helicobacter pylori infection in patients with bleeding ulcer disease: rapid urease test and histology. Rev Esp Enferm Dig 96: 395-398.
18. Gisbert JP, Esteban C, Jimenez I, Moreno-Otero R, (2007) 13C-urea breath test during hospitalization for the diagnosis of Helicobacter pylori infection in peptic ulcer bleeding. Helicobacter 12: 231-237.
19. Gisbert JP, Trapero M, Calvet X, Mendoza J, Quesada M, et al. (2004) Evaluation of three different tests for the detection of stool antigens to diagnose Helicobacter pylori infection in patients with upper gastrointestinal bleeding. Aliment Pharmacol Ther 19: 923-929.
20. Calvet X, Lario S, Ramírez-Lázaro MJ, Montserrat A, Quesada M, Reeves L, et al. (2010) Comparative accuracy of 3 monoclonal stool tests for diagnosis of Helicobacter pylori infection among patients with dyspepsia. Clin Infect Dis 50: 323-8.
21. Calvet X, Sánchez-Delgado J, Montserrat A, Lario S, Ramírez-Lázaro MJ, et al. (2009) Accuracy of diagnostic tests for Helicobacter pylori: a reappraisal. Clin Infect Dis 48: 1385-91.
22. Chomczynski P, Sacchi N, (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
23. Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA, et al. (1986) Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature 324: 163-166.
24. Biswas B, (2006) Assessing agreement for diagnostic devices. FDA/Industry Statistics Workshop. September 28-29, available at: http://www.amstat.org/meetings/fdaworkshop/presentations/2006/Assessing% 20Agreement%20for%20diagnostic%20tests%202006.ppt. Accessed: 15/09/08. 2006.
25. Genta RM, Lash RH, (2010) Helicobacter pylori-negative gastritis: Seek, yet ye shall not always find. Am J Surg Pathol 34: e25-e34.
26. Dixon MF, Genta RM, Yardley JH, Correa P, (1994) Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol 20: 1161-1181.
27. Calvet X, Barkun AN, Kuipers EJ, Lanas A, Bardou M, et al. (2009) Is H. pylori testing clinically useful in the acute setting of upper gastrointestinal bleeding? A systematic review. Gastroenterology 136 supplement 1 T1943.
28. Lanas A, Calvet X, Feu F, Ponce J, Gisbert JP, et al. (2010) First Spanish consensus on peptic ulcer bleeding management. Consenso sobre Hemorragia Digestiva por Úlcera Péptica. Med Clin (Barc) 135: 608-616.
29. Martin de Argila C, Boixeda D, (2001) Practical considerations for the diagnosis of Helicobacter pylori infection [in Spanish]. Med Clin (Barc) 117: 386-391.
30. Houghton J, Ramamoorthy R, Pandya H, Dhirmalani R, Kim KH, (2002) Human plasma is directly bacteriocidal against Helicobacter pylori in vitro, potentially explaining the decreased detection of Helicobacter pylori during acute upper GI bleeding. Gastrointest Endosc 55: 11-16.
31. Leung WK, Sung JJ, Siu KL, Chan FK, Ling TK, et al. (1998) False-negative biopsy urease test in bleeding ulcers caused by the buffering effects of blood. Am J Gastroenterol 93: 1914-1918.
32. Graham DY, Opekun AR, Hammoud F, Yamaoka Y, Reddy R, et al. (2003) Studies regarding the mechanism of false negative urea breath tests with proton pump inhibitors. Am J Gastroenterol 98: 1005-1009.
33. Fantry GT, Rosenstein AH, James SP, (1999) Confounding factors in the detection of Helicobacter pylori infection in patients with upper gastrointestinal bleeding. Am J Gastroenterol 94: 1421-1422.
34. Lage AP, Godfroid E, Fauconnier A, Burette A, Butzler JP, et al. (1995) Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens. J Clin Microbiol 33: 2752-2756.
35. Lin HJ, Lo WC, Perng CL, Tseng GY, Li AF, et al. (2005) Mucosal polymerase chain reaction for diagnosing Helicobacter pylori infection in patients with bleeding peptic ulcers. World J Gastroenterol 11: 382-385.
36. Castillo-Rojas G, Ballesteros MA, Ponce de LS, Leon S, et al. (2002) Bleeding peptic ulcers and presence of Helicobacter pylori by various tests: a case-control study. Eur J Gastroenterol Hepatol 14: 1113-1118.
37. Resti M, Micheli A, Moriondo M, Becciolini L, Cortimiglia M, et al. (2009) Comparison of the effect of antibiotic treatment on the possibility to diagnose invasive pneumococcal disease by cultural or molecular methods: a prospective observational study of children with proven pneumococcal infection. Clin Therapeutics 31: 1266-1273.
38. Lee JM, Breslin NP, Fallon C, O'Morain CA, (2000) Rapid urease tests lack sensitivity in Helicobacter pylori diagnosis when peptic ulcer disease presents with bleeding. Am J Gastroenterol 95: 1166-1170.
39. Wu CY, Poon SK, Chen GH, Chang CS, Yeh HZ, (1999) Interaction between Helicobacter pylori and non-steroidal anti-inflammatory drugs in peptic ulcer bleeding. Scand J Gastroenterol 34: 234-237.
40. Sotoudehmanesh R, Asgari AA, Fakheri HT, Nouraie M, Khatibian M, et al. (2005) Peptic ulcer bleeding: is Helicobacter pylori a risk factor in an endemic area? Indian J Gastroenterol 24: 59-61.
41. Hayat M, Everett S, Breslin NP, Moayyedi P, O'Mahony S, et al. (1999) The prevalence of Helicobacter pylori in bleeding peptic ulcer disease. Gut 45: A77.
42. Pilotto A, Leandro G, Di Mario F, Franceschi M, Bozzola L, et al. (1997) Role of Helicobacter pylori infection on upper gastrointestinal bleeding in the elderly: a case-control study. Dig Dis Sci 42: 586-591.
43. Hung LC, Ching JY, Sung JJ, To KF, Hui AJ, et al. (2005) Long-term outcome of Helicobacter pylori-negative idiopathic bleeding ulcers: a prospective cohort study. Gastroenterology 128: 1845-1850.
44. Arroyo MT, Forne M, de Argila CM, Feu F, Arenas J, et al. (2004) The prevalence of peptic ulcer not related to Helicobacter pylori or non-steroidal anti-inflammatory drug use is negligible in southern Europe. Helicobacter 9: 249-254.
45. Gisbert JP, Gonzalez L, de Pedro A, Valbuena M, Prieto B, et al. (2001) Helicobacter pylori and bleeding duodenal ulcer: prevalence of the infection and role of non-steroidal anti-inflammatory drugs. Scand J Gastroenterol 36: 717-724.