Bacterial biomass production in an estuarine system: High variability of leucine conversion factors and changes in bacterial community structure during incubation

Aquatic Microbial Ecology

Volumn 62, Issue 3, 2011, Pages 299-310

  Baptista, Inês ^a   Luísa Santos, Ana ^a   Cunha, Ângela ^a   Gomes, Newton C M ^a   Almeida, Adelaide ^a  

^a UNIVERSITY OF AVEIRO   (Portugal)

Author keywords

Bacterial c.ommunities.; Conversion f.actors.; Estuary; Heterotrophic bacterioplankton; Isotope d.ilution.; Leucine i.ncorporation.

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 79956325300     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame01472     Document Type: Article

References (70)

1. Almeida MA, Cunha MA, Alcântara F (2001a) Factors influencing bacterial production in a shallow estuarine system. Microb Ecol 42:416-426 (Pubitemid 33141532)
2. Almeida MA, Cunha MA, Alcântara F (2001b) Physiological responses of marine and brackish water bacterial assemblages in a tidal estuary (Ria de Aveiro, Portugal). Aquat Microb Ecol 25:113-125 (Pubitemid 34253484)
3. Almeida MA, Cunha MA, Alcântara F (2002) Seasonal change in the proportion of bacterial and phytoplankton production along a salinity gradient in a shallow estuary. Hydrobiologia 475-476:251-262 (Pubitemid 35288753)
4. Almeida MA, Cunha MA, Alcântara F (2005) Relationship of bacterioplankton production and respiration in a shallow estuarine system (Ria de Aveiro, Portugal). Microbiol Res 160:315-328 (Pubitemid 40793852)
5. Almeida MA, Cunha MA, Dias JM (2007) Bacterial productivity distribution during a rainy year in an estuarine system. Microb Ecol 53:208-220
6. Bååth E (1998) Growth rates of bacterial communities in soils at varying pH: a comparison of the thymidine and leucine incorporation techniques. Microb Ecol 36:316-327 (Pubitemid 29014579)
7. Bell RT, Kuparinen J (1984) Assessing phytoplankton and bacterioplankton production during early spring in Lake Erken, Sweden. Appl Environ Microbiol 48:1221-1230
8. Bjørnsen PK (1986) Automatic determination of bacterioplankton biomass by image analysis. Appl Environ Microbiol 51:1199-1204 (Pubitemid 16068616)
9. Bjørnsen PK, Kuparinen J (1991) Determination of bacterioplankton biomass, net production and growth efficiency in the Southern Ocean. Mar Ecol Prog Ser 71:185-194
10. Bratbak G, Dundas I (1984) Bacterial dry matter content and biomass estimations. Appl Environ Microbiol 48: 755-757 (Pubitemid 14010843)
11. Buesing N, Marxsen J (2005) Theoretical and empirical conversion factors for determinating bacterial production in freshwater sediments via leucine incorporation. Limnol Oceanogr Methods 3:101-107 (Pubitemid 43723680)
12. Calvo-Díaz A, Morán XAG (2009) Empirical leucine-tocarbon conversion factors for estimating heterotrophic bacterial production: seasonality and predictability in a temperate coastal ecosystem. Appl Environ Microbiol 75: 3216-3221
13. Caron DA, Dam HG, Kremer P, Lessard EJ and others (1995) The contribution of microorganisms to particulate carbon and nitrogen in surface waters of the Sargasso Sea near Bermuda. Deep-Sea Res I 42:943-972 (Pubitemid 26450894)
14. Coffin RB (1989) Bacterial uptake of dissolved free and combined amino acids in estuarine waters. Limnol Oceanogr 34:531-542
15. Cole JJ, Pace ML (1995) Why measure bacterial production? A reply to the comment by Jahnke and Craver. Limnol Oceanogr 40:441-444
16. Dias JM, Lopes JF (2006) Implementation and assessment of hydrodynamic, salt and heat transport models: the case of Ria de Aveiro lagoon (Portugal). Environ Model Softw 21: 1-15 (Pubitemid 41736195)
17. Dias JM, Lopes JF, Dekeyser I (2000) Tidal propagation in Ria de Aveiro lagoon, Portugal. Phys Chem Earth B 25: 369-374 (Pubitemid 30319989)
18. Dias JM, Lopes JF, Dekeyser I (2001) Lagrangian transport of particles in Ria de Aveiro lagoon, Portugal. Phys Chem Earth B 26:721-727 (Pubitemid 32793031)
19. 14C] leucine incorporation technique to measure bacterial production in river sediments and the epiphyton. Appl Environ Microbiol 65:4411-4418
20. Fry JC (1988) Determination of biomass. In: Austin B (ed) Methods in aquatic bacteriology. John Wiley, London, p 27-72
21. Fuhrman JA, Azam F (1980) Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl Environ Microbiol 39: 1085-1095 (Pubitemid 10001970)
22. Ghiglione JF, Mevel G, Pujo-Pay M, Mousseau L, Lebaron P, Goutx M (2007) Diel and seasonal variations in abundance, activity, and community structure of particleattached and free-living bacteria in NW Mediterranean Sea. Microb Ecol 54:217-231 (Pubitemid 47197896)
23. 3H]leucine incorporation technique for quantification of bacterial secondary production associated with decaying wetland plant litter. Appl Environ Microbiol 72:5948-5956 (Pubitemid 44414622)
24. Gocke K (1977) Untersuchungen über die heterotrophe Aktivität in der zentralen Ostsee. Mar Biol 40:87-94
25. Gunkel W (1966) Bakteriologische Untersuchungen im Indischen Ozean. Veröff Inst Meeresforsch Bremerh Sonderb 2:255-264
26. Hamasaki K, Taniguchi A, Tada Y, Long RA, Azam F (2007) Actively growing bacteria in the inland sea of Japan, identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis. Appl Environ Microbiol 73:2787-2798 (Pubitemid 46718558)
27. Henriques I, Almeida A, Cunha A, Correia A (2004) Molecular sequence analysis of prokariotic diversity in the micdle and outer sections of the Portuguese estuary Ria de Aveiro. FEMS Microbiol Ecol 49:269-279 (Pubitemid 38925334)
28. Heuer H, Hartung K, Wieland G, Kramer I, Smalla K (1999) Polynocleotide probes that target a hypervariable region of 16S rRNA genes to identify bacterial isolates corresponding to bands of community fingerprints. Appl Environ Microbiol 65:1045-1049
29. Heuer H, Wieland G, Schönfeld J, Schönwälder A, Gomes NCM, Smalla K (2001) Bacterial community profiling using DGGE or TGGE analysis. In: Rouchelle P (ed) Environmental molecular microbiology: protocols and applications. Horizon Scientific Press, Wymondham, UK, p 177-190
30. Higo K, Yamamoto O (1985) Effect of tritiated water on growth of a radiation-sensitive strain of Escherichia coli, NG30. J Radiat Res (Tokyo) 26:353-359
31. Hobbie JE, Daley RJ, Jasper S (1977) Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol 33:1225-1228 (Pubitemid 8108362)
32. 3H]valine into protein of freshwater bacteria: uptake kinetics and intracellular isotope dilution. Appl Environ Microbiol 58:3638-3646
33. Kirchman DL, Ducklow HW (1993) Estimating conversion factors for the thymidine and leucine methods for measuring bacterial production. In: Kemp PF, Sherr BF, Sherr EB, Cole JJ (eds) Handbook of methods in aquatic microbial ecology. Lewis, Boca Raton, FL, p 513-518
34. Kirchman DL, Hoch MP (1988) Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates. Mar Ecol Prog Ser 45:169-178
35. Kirchman D, K'nees E, Hodson R (1985) Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl Environ Microbiol 49:599-607 (Pubitemid 15138048)
36. Kolm HE, Schoenenberger MF, Piemonte MR, Souza PSA, Scühli GS, Mucciatto MB, Mazzuco R (2002) Spatial variation of bacteria in surface waters of Paranaguá and Antonina Bays, Paraná, Brazil. Braz Arch Biol Technol 45:27-34 (Pubitemid 44231903)
37. Lee S, Fuhrman JA (1987) Relationships between biovolume and biomass of naturally derived marine bacterioplankton. Appl Environ Microbiol 53:1298-1303
38. Loferer-Krößbacher M, Klima J, Psenner R (1998) Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol 64:688-694 (Pubitemid 28079842)
39. Lopes JF, Cardoso AC (2010) 3D Ecological modelling of the Aveiro Coast. In: Mihailovic DT, Gualtieri C (eds) Advances in environmental fluid mechanics, Vol 1. World Scientific, Singapore, p 309-326
40. Lopes CB, Pereira ME, Vale C, Lillebø AI, Pardal MA, Duarte AC (2007) Assessment of spatial environmental quality status in Ria de Aveiro (Portugal). Sci Mar 71:293-304 (Pubitemid 47083012)
41. Macko SA, Green EJ (1982) An investigation of the dissolved free amino acids and their relation to phytoplankton cell density in the Damariscotta river estuary, Maine. Estuaries 5:68-73 (Pubitemid 13280298)
42. Martins V, Silva EF, Sequeira C, Rocha F, Duarte AC (2010) Evaluation of the ecological effects of heavy metals on the assemblages of benthic foraminifera of the canals of Aveiro (Portugal). Estuar Coast Shelf Sci 87:293-304
43. Massana R, Pedrós-Alió C, Casamayor EO, Gasol JM (2001) Changes in marine bacterioplankton phylogenetic composition during incubations designed to measure biogeochemically significant parameters. Limnol Oceanogr 46: 1181-1188 (Pubitemid 32665193)
44. Miranda MR, Guimarães JRD, Coelho-Souza AS (2007) [3H]Leucine incorporation method as a tool to measure secondary production by periphytic bacteria associated to the roots of floating aquatic macrophyte. J Microbiol Methods 71:23-31 (Pubitemid 47445806)
45. Moran MA, Hodson RE (1992) Contributions of three subsystems of a freshwater marsh to total bacterial secondary productivity. Microb Ecol 24:161-170
46. Nelson CE, Carlson CA (2005) A nonradioactive assay of bacterial productivity optimized for oligotrophic pelagic environments. Limnol Oceanogr Methods 3:211-220 (Pubitemid 43723666)
47. Norland S (1993) The relationship between biomass and volume of bacteria. In: Kemp PF, Sherr BF, Sherr EB, Cole JJ (eds) Handbook of methods of aquatic microbial ecology. Lewis, Boca Raton, FL, p 303-307
48. Pace ML, Cole JJ (1994) Primary and bacterial production in lakes: are they coupled over depth? J Plankton Res 16: 661-672 (Pubitemid 2090019)
49. Pedrós-Alió C, Calderón-Paz J, Guixa-Boixereu N, Estrada M, Gasol JM (1999) Bacterioplankton and phytoplankton biomass and production during summer stratification in the northwestern Mediterranean Sea. Deep-Sea Res I 46: 985-1019 (Pubitemid 29235637)
50. Pedrós-Alió C, Vaqué D, Guixa-Boixereu N, Gasol JM (2002) Prokaryotic plankton biomass and heterotrophic production in western Antarctic waters during the 1995-1996 Austral summer. Deep-Sea Res II 49:805-825 (Pubitemid 34063224)
51. Person S, Bockrath RC (1964) Differential mutation production by the decay of incorporated tritium compound in Escherichia coli. Biophys J 4:355-365
52. Ramette A (2007) Multivariate analyses in microbial ecology. FEMS Microbiol Ecol 62:142-160 (Pubitemid 47610610)
53. Rheinheimer G (1985) Mikrobiologie der Gewässer. Gustav Fischer, Jena, p 262
54. Riemann B, Søndergaard M (1984) Measurements of diel rates of bacterial secondary production in aquatic environments. Appl Environ Microbiol 47:632-638
55. Riemann B, Newell S, Fallon R (1987) Calculation of cell production of coastal marine bacteria based on measured incorporation of [3H]thymidine. Limnol Oceanogr 32:471-476
56. Riemann B, Bell RT, Jørgensen NOG (1990) Incorporation of thymidine, adenine and leucine into natural bacterial assemblages. Mar Ecol Prog Ser 65:87-94
57. Rivkin RB, Anderson MR (1997) Inorganic nutrient limitation of oceanic bacterioplankton. Limnol Oceanogr 42:730-740 (Pubitemid 27468396)
58. Santos L, Coelho F, Mendes C, Nance L, Santos E, Cunha A, Almeida A (2007) Does photochemical alteration of dissolved organic matter influence the structure and activity of the estuarine prokaryote communities? 10th Symposium on Aquatic Microbial Ecology. SAME 10 Abstract Book. Sep 2-7 2007, Faro, Portugal
59. Sela M, White FH Jr, Anfinsen CB (1957) Reductive cleavage of disulfide bridges in ribonuclease. Science 125:691-692
60. Sheridan GE, Szabo EA, Mackey BM (1999) Effect of posttreatment holding conditions on detection of tufA mRNA in ethanol-treated Escherichia coli: implications for RTPCR-based indirect viability tests. Lett Appl Microbiol 29: 375-379 (Pubitemid 30052884)
61. Silva JJ (1994) Circulação de Água na Ria de Aveiro: Contribuição para o Estudo da Qualidade da Água. PhD thesis, University of Aveiro
62. Simon M (1991) Isotope dilution of intracellular amino acids as a tracer of carbon and nitrogen sources of marine planktonic bacteria. Mar Ecol Prog Ser 74: 295-301
63. Simon M, Azam F (1989) Protein content and protein synthesis rates of planktonic marine bacteria. Mar Ecol Prog Ser 51:201-213
64. Simon M, Rosenstock B (1992) Carbon and nitrogen sources of planktonic bacteria in Lake Constance studied by the composition and isotope dilution of intracellular amino acids. Limnol Oceanogr 37:1496-1511
65. Sintes E, Herndl GJ (2006) Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography. Appl Environ Microbiol 72:7022-7028 (Pubitemid 44748414)
66. Smalla K, Wieland G, Buchner A, Zock A and others (2001) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Appl Environ Microbiol 67:4742-4751 (Pubitemid 33646108)
67. Suzuki MT (1999) Effect of protistan bacterivory on coastal bacterioplankton diversity. Aquat Microb Ecol 20: 261-272 (Pubitemid 34234410)
68. Valencia J, Abalde J, Bode A, Cid A and others (2003) Variations in planktonic bacterial biomass production and phytoplankton blooms off A Coruña (NW Spain). Sci Mar 67:143-157 (Pubitemid 36967486)
69. van Looij A, Riemann B (1993) Measurements of bacterial production in coastal marine environments using leucine: application of a kinetic approach to correct for isotope dilution. Mar Ecol Prog Ser 102:97-104
70. Vila M, Simó R, Kiene RP, Pinhassi J, González JM, Moran MA, Pedrós-Alió C (2004) Use of microautoradiography combined with fluorescence in situ hybridization to determine dimethylsulfoniopropionate incorporation by marine bacterioplankton taxa. Appl Environ Microbiol 70: 4648-4657 (Pubitemid 39095488)