Molecular discrimination of sheep and cattle isolates of Echinococcus granulosus by SSCP and conventional PCR in Turkey

Veterinary Parasitology

Volumn 178, Issue 3-4, 2011, Pages 367-369

  Simsek, Sami ^a   Balkaya, Ibrahim ^b   Ciftci, Ayşe Turkan ^c   Utuk, Armagan Erdem ^d  

^a FIRAT UNIVERSITY   (Turkey)

^b ATATÜRK UNIVERSITY   (Turkey)

^c Veterinary Control and Research Institute   (Turkey)

^d Etlik Central Veterinary Control and Research Institute   (Turkey)

Author keywords

Cattle; Echinococcus granulosus; Genotype; PCR; Sheep; SSCP; Turkey

Indexed keywords

CYTOCHROME C OXIDASE; CYTOCHROME C OXIDASE SUBUNIT 1; GENOMIC DNA; RIBOSOME RNA; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; DNA ISOLATION; DNA POLYMORPHISM; ECHINOCOCCUS GRANULOSUS; GENE AMPLIFICATION; GENOTYPE; LIVER; LUNG; MITOCHONDRIAL GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RNA GENE; SAMPLING; SHEEP; SINGLE STRANDED CONFORMATIONAL POLYMORPHISM; TURKEY (REPUBLIC);

ANIMALS; CATTLE; CATTLE DISEASES; DNA, MITOCHONDRIAL; DNA, PROTOZOAN; ECHINOCOCCOSIS; ECHINOCOCCUS GRANULOSUS; ELECTRON TRANSPORT COMPLEX IV; GENETIC VARIATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL; SHEEP; SHEEP DISEASES; TURKEY;

ANIMALIA; BOS; ECHINOCOCCUS GRANULOSUS; OVIS ARIES;

EID: 79956276856     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2011.01.033     Document Type: Article

References (17)

1. Bowles J., Blair D., McManus D.P. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol. Biochem. Parasitol. 1992, 54:165-173.
2. Casulli A., Manfredi M.T., La Rosa G., Cerbo A.R., Genchi C., Pozio E. Echinococcus ortleppi and E. granulosus G1, G2 and G3 genotypes in Italian bovines. Vet. Parasitol. 2008, 155:168-172.
3. Dinkel A., Njoroge E.M., Zimmermann A., Walz M., Zeyhle E., Elmahdi I.E., Mackenstedt U., Romig T. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int. J. Parasitol. 2004, 34:645-653.
4. Gasser R.B., Zhu X., McManus D.P. Display of sequence variation in PCR-amplified mitochondrial DNA regions of Echinococcus by single-strand conformation polymorphism. Acta Trop. 1998, 71:107-115.
5. Lavikainen A., Haukisalmi V., Lehtinen M.J., Henttonen H., Oksanen A., Meri S. A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 2008, 135:1457-1467.
6. Moks E., Jõgisalu I., Valdmann H., Sarma U. First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of 'genotypes' G5-G10. Parasitology 2008, 135:647-654.
7. Nakao M., Yanagida T., Okamoto M., Knapp J., Nkouawa A., Sako Y., Ito A. State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect. Genet. Evol. 2010, 10:444-452.
8. Nataraj A.J., Olivos-Glander I., Kusukawa N., Highsmith W.E. Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection. Electrophoresis 1999, 20:1177-1185.
9. Saarma U., Jõgisalu I., Moks E., Varcasia A., Lavikainen A., Oksanen A., Simsek S., Andresiuk V., Rinaldi L., González L.M., Ferrer E., Gárate T., Maravilla P. A novel phylogeny for the genus Echinococcus based on nuclear data challenges relationships based on mitochondrial evidence. Parasitology 2009, 136:317-328.
10. Simsek S., Balkaya I., Koroglu E. Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet. Parasitol. 2010, 172:347-349.
11. Simsek S., Eroksuz Y. Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop. 2009, 109:167-169.
12. Simsek S., Koroglu E., Dumanli N., Aktas M., Saki C.E., Altay K., Utuk A.E. Seroprevalance of cattle hydatidosis in some districts in the east Anatolian region of Turkey. Turk. J. Vet. Anim. Sci. 2005, 29:1305-1310.
13. Snábel V., Altintas N., D'Amelio S., Nakao M., Romig T., Yolasigmaz A., Gunes K., Turk M., Busi M., Hüttner M., Sevcová D., Ito A., Altintas N., Dubinský P. Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol. Res. 2009, 105:145-154.
14. Thompson R.C.A., McManus D.P. Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002, 18:452-457.
15. Utuk A.E., Simsek S., Koroglu E., McManus D.P. Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop. 2008, 107:192-194.
16. Vural G., Baca A.U., Gauci C.G., Bagci O., Gicik Y., Lightowlers M.W. Variability in the Echinococcus granulosus cytochrome c oxidase 1 mitochondrial gene sequence from livestock in Turkey and a reappraisal of the G1-3 genotype cluster. Vet. Parasitol. 2008, 154:347-350.
17. Zhu X.Q., Jacobs D.E., Chilton N.B., Sani R.A., Cheng N.A.B.Y., Gasser R.B. Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia. Parasitology 1998, 117:155-164.