Imaging NF-κB Signaling in Mice for Screening Anticancer Drugs

Methods in Molecular Biology

Volumn 716, Issue , 2011, Pages 169-177

  Robbins, Delira ^a   Zhao, Yunfeng ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Anticancer drugs; Bioluminescence; Bioluminescence imaging; Luciferase; NF B

Indexed keywords

ANTINEOPLASTIC AGENT; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LUCIFERASE;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; DRUG SCREENING; GENE EXPRESSION REGULATION; GENETICS; LUMINESCENCE; MALE; METABOLISM; METHODOLOGY; MOUSE; TRANSGENIC MOUSE;

ANIMALS; ANTINEOPLASTIC AGENTS; DRUG SCREENING ASSAYS, ANTITUMOR; GENE EXPRESSION REGULATION; LUCIFERASES; LUMINESCENCE; MALE; MICE; MICE, INBRED BALB C; MICE, TRANSGENIC; NF-KAPPA B;

EID: 79956206339     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-012-6_10     Document Type: Chapter

References (11)

1. Contag, C.H., Jenkins, D.,Contag P.R., and Negrin, R.S. (2000) Use of reporter genes for optical measurements of neoplastic diseases In vivo. Neoplasia 2: 41–52.
2. Klerk, C.P., Overmeer, R.M., Niers, T.M.H., Versteeg, H.H., Richel, D.J., Buckle, T., et al. (2007) Validity of bioluminescence measurements for noninvasive in vivo imaging of tumor load in small animals. Biotechniques 43: 7–13.
3. Sadikot, R.T. and Blackwell, T.S. (2005) Bioluminescence imaging. Proc Am Thorac Soc 2: 537–540.
4. Sato, A., Klaunberg, B., and Tolwani, R. (2004) In vivo bioluminescence imaging. Comp Med 54: 631–634.
5. Zangani, M., Carlsen, H., Kielland, A., Os, A., Hauglin, H., Blomhoff, R., et al. (2009) Tracking early autoimmune disease by biolu-minescent imaging of NF-kB activation reveals pathology in multiple organ systems. Am J Pathol 174: 1358–1367.
6. Kim, S.B., Ozawa, T., Walanabe, S., and Umezawa, Y. (2004) High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proc Natl Acad Sci USA 101: 11542–11547.
7. Ondrey, F., Dong, G., Sunwoo, J., Chen, Z., Wolf, J.S., Crowl-Bancroft, C.V., et al. (1999) Constitutive activation of transcription factors NF-kB, AP-1 and NF-IL6 in human head and neck squamous cell carcinoma cell lines that express proinflammatory and proangiogenic cytokines. Mol Carcinog 26: 119–129.
8. Baldwin, A.S. (1996) The NF-kB and IkB proteins: New discoveries and insights. Annu Rev Immunol 14: 649–683.
9. He, Y., Zeng, Q., Drenning, S.D., Melhem, M.F., Tweardy, D.J., Huang, L., et al. (1998) Inhibition of human squamous cell carcinoma growth in vivo by epidermal growth factor receptor antisense RNA transcribed from the U6 promoter. J Natl Cancer Inst 90: 1080–1087.
10. Sun, L. and Carpenter, G. (1998) Epidermal growth factor activation of NF-kB is mediated through IkBa degradation and intracellular free calcium. Oncogene 16: 2095–2102.
11. Xenogen Corporation. (2006) Xenogen Living Image Software Manual 2.6. Xenogen Corporation, San Francisco, CA.