Tandem mass spectrometric method for definitive localization of phosphorylation sites using bromine signature

Analytical Biochemistry

Volumn 414, Issue 2, 2011, Pages 294-296

  Kim, Jong Seo ^a,b   Kim, Jisoo ^a   Oh, Jung Min ^a   Kim, Hie Joon ^a  

^a Seoul National University   (South Korea)

^b PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADDITION REACTIONS; AMINO ACIDS; BROMINE; ISOTOPES; MASS SPECTROMETRY; PEPTIDES;

BROMINE SIGNATURES; ISOTOPIC SIGNATURES; MICHAEL ADDITIONS; PHOSPHOPEPTIDE ENRICHMENT; PHOSPHORYLATION SITES; TANDEM MASS SPECTROMETRIC; TANDEM MASS SPECTROMETRY; THREONINE RESIDUES;

PHOSPHORYLATION;

4 BROMOBENZYLMERCAPTAN; BROMINE; PHOSPHATE; PHOSPHOPEPTIDE; PHOSPHOSERINE; PHOSPHOTHREONINE; SERINE; THIOL DERIVATIVE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CHEMICAL STRUCTURE; MASS SPECTROMETRY; MICHAEL ADDITION; PEPTIDE SYNTHESIS; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; TANDEM MASS SPECTROMETRY;

EID: 79956160456     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.032     Document Type: Article

References (14)

1. S.K. Hanks, A.M. Quinn, T. Hunter, The protein kinase family: conserved features and deduced phylogeny of the catalytic domains, Science 241 (1988) 42-52.
2. T. Hunter, Signaling - 2000 and beyond, Cell 100 (2000) 113-127. (Pubitemid 30046300)
3. R. Aebersold, D.R. Goodlett, Mass spectrometry in proteomics, Chem. Rev. 101 (2001) 269-295.
4. J.D. Dunn, G.E. Reid, M.L. Bruening, Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry, Mass Spectrom. Rev. 29 (2010) 29-54.
5. D.J. States, G.S. Omenn, T.W. Blackwell, D. Fermin, J. Eng, D.W. Speicher, S.M. Hanash, Challenges in deriving high-confidence protein identifications from data gathered by a HUPO plasma proteome collaborative study, Nat. Biotechnol. 24 (2006) 333-338.
6. D.M. Good, M. Wirtala, G.C. McAlister, J.J. Coon, Performance characteristics of electron transfer dissociation mass spectrometry, Mol. Cell. Proteomics 6 (2007) 1942-1951.
7. C. Klemm, S. Schroder, M. Gluckmann, M. Beyermann, E. Krause, Derivatization of phosphorylated peptides with S- and N-nucleophiles for enhanced ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry, Rapid Commun. Mass Spectrom. 18 (2004) 2697-2705.
8. G. Arrigoni, S. Resjo, F. Levander, R. Nilsson, E. Degerman, M. Quadroni, L.A. Pinna, P. James, Chemical derivatization of phosphoserine and phosphothreonine containing peptides to increase sensitivity for MALDI-based analysis and for selectivity of MS/MS analysis, Proteomics 6 (2006) 757-766. (Pubitemid 43260201)
9. Y.H. Ahn, E.S. Ji, K.H. Kwon, J.Y. Lee, K. Cho, J.Y. Kim, H.J. Kang, H.G. Kim, J.S. Yoo, Protein phosphorylation analysis by site-specific arginine-mimic labeling in gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Anal. Biochem. 370 (2007) 77-86.
10. M.N. Masaru Miyagi, T. Nakazawa, I. Kato, S. Tsunasawa, A novel derivatization method with 5-bromonicotinic acid N-hydroxysuccinimide for determination of the amino acid sequences of peptides, Rapid Commun. Mass Spectrom. 12 (1998) 603-608.
11. J.-S. Kim, J.-Y. Kim, H.-J. Kim, Suppression of matrix clusters and enhancement of peptide signals in MALDI-TOF mass spectrometry using nitrilotriacetic acid, Anal. Chem. 77 (2005) 7483-7488.
12. J.-S. Kim, E. Cui, H.-J. Kim, Picolinamidination of phosphopeptides for MALDI-TOF-TOF mass spectrometric sequencing with enhanced sensitivity, J. Am. Soc. Mass Spectrom. 20 (2009) 1751-1758.
13. J. Hedou, B. Bastide, A. Page, J.C. Michalski, W. Morelle, Mapping of O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle, Proteomics 9 (2009) 2139-2148.
14. 2 enrichment for phosphopeptide analysis by liquid chromatography/tandem mass spectrometry, Rapid Commun. Mass Spectrom. 24 (2010) 1467-1474.