Specific allowance for antibody bivalence in the determination of dissociation constants by kinetic exclusion assay

Analytical Biochemistry

Volumn 414, Issue 2, 2011, Pages 273-277

  Winzor, Donald J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Antibody bivalence; Antigen antibody interaction; Binding affinity; Kinetic exclusion assay; Solid phase immunoassay

Indexed keywords

ANTIGENS; BINDING ENERGY; DISSOCIATION; ETHYLENEDIAMINETETRAACETIC ACID; KINETICS;

ANALYTICAL PROCEDURE; ANTIBODY CAPTURE; ANTIGEN-ANTIBODY INTERACTION; BINDING AFFINITIES; DISSOCIATION CONSTANT; EXTRACELLULAR DOMAINS; HUMAN INSULINS; SOLID-PHASE IMMUNOASSAY;

ANTIBODIES;

CADMIUM; EDETIC ACID; IMMUNOGLOBULIN G; SOMATOMEDIN;

ANTIBODY DETECTION; ANTIGEN ANTIBODY REACTION; ARTICLE; CONCENTRATION (PARAMETERS); DISSOCIATION CONSTANT; IMMUNOASSAY; KINETIC EXCLUSION ASSAY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; QUANTITATIVE ANALYSIS;

EID: 79956141263     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.025     Document Type: Article

References (16)

1. D.A. Blake, P. Chakrabarti, M. Khosraviani, F.M. Hatcher, C.M. Westhoff, P. Goebel, D.E. Wylie, R.C. Blake, Metal binding properties of a monoclonal antibody directed toward metal-chelate complexes, J. Biol. Chem. 271 (1996) 27677-27685.
2. D.A. Blake, R.C. Blake, M. Khosraviani, A.R. Pavlov, Immunoassays for metal ions, Clin. Chim. Acta 376 (1998) 13-19.
3. R.C. Blake, A.R. Pavlov, D.A. Blake, Assays to quantify protein binding interactions in homogeneous solution, Anal. Biochem. 272 (1999) 123-134.
4. M. Khosraviani, R.C. Blake, A.R. Pavlov, S.C. Lorbach, H. Yu, J.B. Delehanty, M.W. Brechbiel, D.A. Blake, Binding properties of a monoclonal antibody directed toward lead-chelate complexes, Bioconjug. Chem. 11 (2000) 267-277.
5. N. Ohmura, S.J. Lackie, H. Saiki, An immunoassay for small analytes with theoretical detection limits, Anal. Chem. 73 (2001) 3392-3399.
6. R.M. Jones, H. Yu, J.B. Delehanty, D.A. Blake, Monoclonal antibodies that recognize minimal differences in the three-dimensional structure of metal-chelate complexes, Bioconjug. Chem. 13 (2002) 408-415.
7. J.B. Delehanty, R.M. Jones, T.C. Bishop, D.A. Blake, Identification of important residues in metal-chelate recognition by monoclonal antibodies, Biochemistry 42 (2003) 14173-14183. (Pubitemid 37499414)
8. R.J. Darling, P.-A. Brault, Kinetic exclusion assay technology: characterization of molecular interactions, Assay Drug Dev. Technol. 2 (2004) 647-657.
9. L. Xie, R.M. Jones, T.R. Glass, R. Navoa, Y. Wang, M.J. Grace, Measurement of the functional affinity constant of a monoclonal antibody for cell surface receptors using kinetic exclusion fluorescence immunoassay, J. Immunol. Methods 304 (2005) 1-14. (Pubitemid 41262216)
10. P.J. Flory, Molecular size distribution in three-dimensional polymers: I. Gelation, J. Am. Chem. Soc. 63 (1941) 3083-3090.
11. P.D. Calvert, L.W. Nichol, W.H. Sawyer, Binding equations for interacting systems comprising multivalent acceptor and bivalent antigen-antibody systems, J. Theor. Biol. 80 (1981) 233-247.
12. L.W. Nichol, L.D. Ward, D.J. Winzor, Multivalency of the partitioning species in quantitative affinity chromatography: evaluation of the site-binding constant for the aldolase-phosphate interaction from studies with cellulose phosphate as the affinity matrix, Biochemistry 20 (1981) 4856-4860.
13. I.M. Klotz, The application of the law of mass action to binding by proteins: interactions with calcium, Arch. Biochem. 9 (1946) 109-117.
14. D.J. Winzor, Allowance for antibody bivalence in the characterization of interactions by ELISA, J. Mol. Recognit. 24 (2011) 139-148.
15. B. Friguet, A.F. Chaffotte, L. Djavadi-Ohaniance, M.E. Goldberg, Measurement of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay, J. Immunol. Methods 77 (1985) 305-319. (Pubitemid 15103678)
16. P.J. Hogg, S.C. Johnstone, M.R. Bowles, S.E. Pond, D.J. Winzor, Evaluation of equilibrium constants by solid-phase immunoassay: the binding of paraquat to its elicited mouse monoclonal antibody, Mol. Immunol. 24 (1987) 797-801.