Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI

Journal of Bacteriology

Volumn 193, Issue 10, 2011, Pages 2418-2428

  Rhodes, Ryan G ^a   Pucker, Halley G ^a   McBride, Mark J ^a  

^a UNIVERSITY OF WISCONSIN MILWAUKEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHITIN; STREPTOMYCIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL CELL; BACTERIOPHAGE; BACTERIUM COLONY; BACTERIUM ISOLATION; CELL MOTILITY; CELL MUTANT; CONTROLLED STUDY; FLAVOBACTERIUM JOHNSONIAE; GENE DELETION; GENE SEQUENCE; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; SUICIDE GENE; WESTERN BLOTTING;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DNA MUTATIONAL ANALYSIS; DRUG RESISTANCE, BACTERIAL; FLAVOBACTERIUM; GENES, BACTERIAL; GENETIC VECTORS; GENETICS, MICROBIAL; LOCOMOTION; PLASMIDS; RECOMBINATION, GENETIC; RIBOSOMAL PROTEINS; SELECTION, GENETIC; SEQUENCE DELETION; STREPTOMYCIN;

FLAVOBACTERIUM JOHNSONIAE;

EID: 79956069357     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00117-11     Document Type: Article

References (34)

1. Agarwal, S., D. W. Hunnicutt, and M. J. McBride. 1997. Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA. Proc. Natl. Acad. Sci. U. S. A. 94:12139-12144.
2. Alvarez, B., P. Secades, M. J. McBride, and J. A. Guijarro. 2004. Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum. Appl. Environ. Microbiol. 70:581-587.
3. Bolivar, F., and K. Backman. 1979. Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 68:245-267.
4. Braun, T. F., M. K. Khubbar, D. A. Saffarini, and M. J. McBride. 2005. Flavobacterium johnsoniae gliding motility genes identified by mariner mutagenesis. J. Bacteriol. 187:6943-6952.
5. Braun, T. F., and M. J. McBride. 2005. Flavobacterium johnsoniae GldJ is a lipoprotein that is required for gliding motility. J. Bacteriol. 187:2628-2637.
6. Chang, L. Y. E., J. L. Pate, and R. J. Betzig. 1984. Isolation and characterization of nonspreading mutants of the gliding bacterium Cytophaga johnsonae. J. Bacteriol. 159:26-35.
7. Chen, S., M. Bagdasarian, M. G. Kaufman, A. K. Bates, and E. D. Walker. 2007. Mutational analysis of the ompA promoter from Flavobacterium johnsoniae. J. Bacteriol. 189:5108-5118.
8. Drecktrah, D., J. M. Douglas, and D. S. Samuels. 2010. Use of rpsL as a counterselectable marker in Borrelia burgdorferi. Appl. Environ. Microbiol. 76:985-987.
9. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. U. S. A. 76:1648-1652.
10. Funatsu, G., and H. G. Wittmann. 1972. Ribosomal proteins. XXXIII. Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin. J. Mol. Biol. 68:547-550.
11. Holland, I. B. 2010. The extraordinary diversity of bacterial protein secretion mechanisms. Methods Mol. Biol. 619:1-20.
12. Hunnicutt, D. W., M. J. Kempf, and M. J. McBride. 2002. Mutations in Flavobacterium johnsoniae gldF and gldG disrupt gliding motility and interfere with membrane localization of GldA. J. Bacteriol. 184:2370-2378.
13. Hunnicutt, D. W., and M. J. McBride. 2001. Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldD and gldE. J. Bacteriol. 183:4167-4175.
14. Hunnicutt, D. W., and M. J. McBride. 2000. Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldB and gldC. J. Bacteriol. 182:911-918.
15. Jarrell, K. F., and M. J. McBride. 2008. The surprisingly diverse ways that prokaryotes move. Nat. Rev. Microbiol. 6:466-476.
16. Kempf, M. J., and M. J. McBride. 2000. Transposon insertions in the Fla-vobacterium johnsoniae ftsX gene disrupt gliding motility and cell division. J. Bacteriol. 182:1671-1679.
17. Li, L.-Y., N. B. Shoemaker, and A. A. Salyers. 1995. Location and characterization of the transfer region of a Bacteroides conjugative transposon and regulation of the transfer genes. J. Bacteriol. 177:4992-4999.
18. Liu, J., M. J. McBride, and S. Subramaniam. 2007. Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. J. Bacteriol. 189:7503-7506.
19. Matsuoka, M., K. Takahama, and T. Ogawa. 2001. Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rps12 gene that allows selection of mutants free from drug resistance markers. Microbiology 147:2077-2087.
20. McBride, M. J. 2001. Bacterial gliding motility: multiple mechanisms for cell movement over surfaces. Annu. Rev. Microbiol. 55:49-75.
21. McBride, M. J., and T. F. Braun. 2004. GldI is a lipoprotein that is required for Flavobacterium johnsoniae gliding motility and chitin utilization. J. Bacteriol. 186:2295-2302.
22. McBride, M. J., T. F. Braun, and J. L. Brust. 2003. Flavobacterium johnsoniae GldH is a lipoprotein that is required for gliding motility and chitin utilization. J. Bacteriol. 185:6648-6657.
23. McBride, M. J., and M. J. Kempf. 1996. Development of techniques for the genetic manipulation of the gliding bacterium Cytophaga johnsonae. J. Bacteriol. 178:583-590.
24. McBride, M. J., et al. 2009. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl. Environ. Microbiol. 75:6864-6875.
25. Nelson, S. S., S. Bollampalli, and M. J. McBride. 2008. SprB is a cell surface component of the Flavobacterium johnsoniae gliding motility machinery. J. Bacteriol. 190:2851-2857.
26. Nelson, S. S., P. P. Glocka, S. Agarwal, D. P. Grimm, and M. J. McBride. 2007. Flavobacterium johnsoniae SprA is a cell-surface protein involved in gliding motility. J. Bacteriol. 189:7145-7150.
27. Pate, J. L., S. J. Petzold, and L.-Y. E. Chang. 1979. Phages for the gliding bacterium Cytophaga johnsonae that infect only motile cells. Curr. Microbiol. 2:257-262.
28. Rhodes, R. G., S. S. Nelson, S. Pochiraju, and M. J. McBride. 2011. Flavobacterium johnsoniae sprB is part of an operon spanning the additional gliding motility genes sprC, sprD, and sprF. J. Bacteriol. 193:599-610.
29. Rhodes, R. G., et al. 2010. Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB. J. Bacteriol. 192:1201-1211.
30. Russell, C. B., and F. W. Dahlquist. 1989. Exchange of chromosomal and plasmid alleles in Escherichia coli by selection for loss of a dominant antibiotic sensitivity marker. J. Bacteriol. 171:2614-2618.
31. +: a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:991-1000.
32. Sato, K., et al. 2010. A protein secretion system linked to bacteroidete gliding motility and pathogenesis. Proc. Natl. Acad. Sci. U. S. A. 107:276-281.
33. Stibitz, S., W. Black, and S. Falkow. 1986. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50:133-140.
34. Wolkin, R. H., and J. L. Pate. 1985. Selection for nonadherent or nonhydrophobic mutants co-selects for nonspreading mutants of Cytophaga johnsonae and other gliding bacteria. J. Gen. Microbiol. 131:737-750.