A novel isolation method of Brucella species and molecular tracking of Brucella suis biovar 2 in domestic and wild animals

Veterinary Microbiology

Volumn 150, Issue 3-4, 2011, Pages 405-410

  Abril, Carlos ^a   Thomann, Andreas ^a   Brodard, Isabelle ^a   Wu, Natacha ^b   Ryser Degiorgis, Marie Pierre ^b   Frey, Joachim ^b   Overesch, Gudrun ^a  

^a Centre for Zoonoses   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Brucella suis; Brucellosis; Domestic pigs; Isolation methods; MLVA 16; Outbreak; Wild boars

Indexed keywords

BACTERIAL DNA; BACTERIUM ANTIBODY;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY DETECTION; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIOTYPE; BRUCELLA; BRUCELLA SUIS; BRUCELLOSIS; CLUSTER ANALYSIS; CONTROLLED STUDY; DOMESTIC PIG; EPIDEMIC; GENETIC PROCEDURES; GENETIC SIMILARITY; GENOTYPE; HARE; MULTILOCUS VARIABLE NUMBER OF TANDEM REPEAT ANALYSIS; NONHUMAN; PHYLOGENETIC TREE; REAL TIME POLYMERASE CHAIN REACTION; SWITZERLAND; VARIABLE NUMBER OF TANDEM REPEAT; WILD BOAR;

ANIMALS; ANIMALS, WILD; BACTERIAL TYPING TECHNIQUES; BACTERIOLOGICAL TECHNIQUES; BRUCELLA; BRUCELLA SUIS; BRUCELLOSIS; DISEASE OUTBREAKS; MINISATELLITE REPEATS; PHYLOGENY; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES; SWITZERLAND;

ANIMALIA; BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS BIOVAR SUIS; LEPUS; SUIDAE; SUS; SUS SCROFA; SUS SCROFA DOMESTICA;

EID: 79955980372     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.02.056     Document Type: Article

References (22)

1. Al Dahouk S., Fleche P.L., Nockler K., Jacques I., Grayon M., Scholz H.C., Tomaso H., Vergnaud G., Neubauer H. Evaluation of Brucella MLVA typing for human brucellosis. J. Microbiol. Methods 2007, 69:137-145.
2. Alvarez J., Saez J.L., Garcia N., Serrat C., Perez-Sancho M., Gonzalez S., Ortega M.J., Gou J., Carbajo L., Garrido F., Goyache J., Dominguez L. Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain. Res. Vet. Sci. 2010.
3. Bricker B.J. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 2002, 90:435-446.
4. Cvetnic Z., Spicic S., Curic S., Jukic B., Lojkic M., Albert D., Thiebaud M., Garin-Bastuji B. Isolation of Brucella suis biovar 3 from horses in Croatia. Vet. Rec. 2005, 156:584-585.
5. Cvetnic Z., Spicic S., Toncic J., Majnaric D., Benic M., Albert D., Thiebaud M., Garin-Bastuji B. Brucella suis infection in domestic pigs and wild boar in Croatia. Rev. Sci. Tech. 2009, 28:1057-1067.
6. De Santis R., Ciammaruconi A., Faggioni G., D'Amelio R., Marianelli C., Lista F. Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis. BMC Microbiol. 2009, 9:66.
7. Demirdal T., Demirturk N. Laboratory-acquired brucellosis. Ann. Acad. Med. Singapore 2008, 37:86-87.
8. Ewalt D.R. Comparison of three culture techniques for the isolation of Brucella abortus from bovine supramammary lymph nodes. J. Vet. Diagn. Invest. 1989, 1:227-230.
9. Garcia-Yoldi D., Le Fleche P., de Miguel M.J., Munoz P.M., Blasco J.M., Cvetnic Z., Marin C.M., Vergnaud G., Lopez-Goni I. Comparison of multiple-locus variable-number tandem-repeat analysis with other PCR-based methods for typing Brucella suis isolates. J. Clin. Microbiol. 2007, 45:4070-4072.
10. Hinic V., Brodard I., Thomann A., Cvetnic Z., Makaya P.V., Frey J., Abril C. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J. Microbiol. Methods 2008, 75:375-378.
11. Hinic V., Brodard I., Thomann A., Holub M., Miserez R., Abril C. IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. BMC Vet. Res. 2009, 5:22.
12. Kattar M.M., Jaafar R.F., Araj G.F., Le Fleche P., Matar G.M., Abi R.R., Khalife S., Vergnaud G. Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity. J. Clin. Microbiol. 2008, 46:3935-3940.
13. Le Fleche P., Jacques I., Grayon M., Al Dahouk S., Bouchon P., Denoeud F., Nockler K., Neubauer H., Guilloteau L.A., Vergnaud G. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol. 2006, 6:9.
14. Leuenberger R., Boujon P., Thür B., Miserez R., Garin-Bastuji B., Rüfenacht J., Stärk K.D. Prevalence of classical swine fever, Aujeszky's disease and brucellosis in a population of wild boar in Switzerland. Vet. Rec. 2007, 160:362-368.
15. Mantur B.G., Mangalgi S.S. Evaluation of conventional castaneda and lysis centrifugation blood culture techniques for diagnosis of human brucellosis. J. Clin. Microbiol. 2004, 42:4327-4328.
16. Munoz P.M., Boadella M., Arnal M., de Miguel M.J., Revilla M., Martinez D., Vicente J., Acevedo P., Oleaga A., Ruiz-Fons F., Marin C.M., Prieto J.M., de la F.J., Barral M., Barberan M., de Luco D.F., Blasco J.M., Gortazar C. Spatial distribution and risk factors of brucellosis in Iberian wild ungulates. BMC Infect. Dis. 2010, 10:46.
17. Navarro E., Casao M.A., Solera J. Diagnosis of human brucellosis using PCR. Expert Rev. Mol. Diagn. 2004, 4:115-123.
18. Office International des Epizooties O.I.E. Manual of Standards for Diagnostic Tests and Vaccines 2008, Office International des Epizooties O.I.E., Paris. 6th ed.
19. Office International des Epizooties O.I.E., 2009a. Bovine brucellosis (NB: Version adopted in May 2009). In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. pp. 1-35.
20. Office International des Epizooties O.I.E., 2009b. Porcine and rangiferine brucellosis: Brucella suis. http://www.cfsph.iastate.edu/Factsheets/pdfs/brucellosis_suis.pdf.
21. Pappas G., Akritidis N., Bosilkovski M., Tsianos E. Brucellosis. N. Engl. J. Med. 2005, 352:2325-2336.
22. Yagupsky P., Peled N. Use of the Isolator 1.5 microbial tube for detection of Brucella melitensis in synovial fluid. J. Clin. Microbiol. 2002, 40:3878.