Improvement of HRV quantification using cRNA-based standards for real time RT-PCR

Molecular Biotechnology

Volumn 48, Issue 1, 2011, Pages 15-18

  Terlizzi, Maria Elena ^a   Bergallo, Massimiliano ^a   Astegiano, Sara ^a   Sidoti, Francesca ^a   Gambarino, Stefano ^a   Solidoro, Paolo ^a   Costa, Cristina ^a   Cavallo, Rossana ^a  

^a MOLINETTE HOSPITAL   (Italy)

Author keywords

BAL; cRNA; Human Rhinovirus; Real Time RT PCR

Indexed keywords

BAL; CRNA; DYNAMIC RANGE; HUMAN RHINOVIRUS; LIMIT OF DETECTION; REAL TIME RT-PCR; RNA VIRUS;

DNA; RNA; STANDARDS; TRANSCRIPTION; VIRUSES;

POLYMERASE CHAIN REACTION;

COMPLEMENTARY RNA;

ARTICLE; GENETIC ANALYSIS; GENETIC VARIABILITY; LUNG LAVAGE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RHINOVIRUS; RNA VIRUS;

REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHINOVIRUS; RNA, COMPLEMENTARY;

HUMAN RHINOVIRUS SP.; RNA VIRUSES;

EID: 79955922891     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9343-9     Document Type: Article

References (9)

1. JW Romano B van Gemen T Kievits 1996 NASBA: A novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements Clinical Laboratory Medicine 16 89 103 1:STN:280:DyaK2s%2FitFahtw%3D%3D (Pubitemid 26087052)
2. P Gill A Ghaemi 2008 Nucleic acid isothermal amplification technologies: A review Nucleosides Nucleotides Nucleic Acids 27 224 243 10.1080/ 15257770701845204 1:CAS:528:DC%2BD1cXhs1WmsLw%3D (Pubitemid 351220650)
3. MJ Espy JR Uhl LM Sloan SP Buckwalter MF Jones EA Vetter JD Yao NL Wengenack JE Rosenblatt FR Cockerill TF Smith 2006 Real-time PCR in clinical microbiology: Applications for routine laboratory testing Clinical Microbiology Reviews 19 165 256 10.1128/CMR.19.1.165-256.2006 1:CAS:528:DC%2BD28XhsFGjur4%3D (Pubitemid 43158452)
4. HGM Niesters 2004 Molecular and diagnostic clinical virology in real time Clinical Microbiology and Infection 10 5 11 10.1111/j.1469-0691.2004.00699.x 1:CAS:528:DC%2BD2cXhsVGnsbg%3D (Pubitemid 38128587)
5. S Fronhoffs G Totzke S Stier N Wernert M Rothe T Brüning B Koch A Sachinidis H Vetter Y Ko 2002 A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction Molecular and Cellular Probes 16 99 110 10.1006/mcpr.2002.0405 1:CAS:528:DC%2BD38XjvFOgtrs%3D (Pubitemid 34712934)
6. I Monne S Ormelli A Salviato C De Battisti F Bettini A Salomoni A Drago B Zecchin I Capua G Cattoli 2008 Development and validation of a one-step real-time PCR assay for simultaneous detection of subtype H5, H7, and H9 avian influenza viruses Journal of Clinical Microbiology 46 1769 1773 10.1128/JCM.02204-07 1:CAS:528:DC%2BD1cXmvV2mt70%3D (Pubitemid 351657227)
7. S Gambarino C Costa M Elia F Sidoti S Mantovani V Gruosso M Bergallo R Cavallo 2009 Development of a RT real-time PCR for the detection and quantification of human rhinoviruses Molecular Biotechnology 42 350 357 10.1007/s12033-009-9164-x 1:CAS:528:DC%2BD1MXns1Cnsbw%3D
8. M Raymaekers R Smets B Maes R Cartuyvels 2009 Checklist for optimization and validation of real-time PCR assays Journal of Clinical Laboratory Analysis 23 145 151 10.1002/jcla.20307 1:CAS:528:DC%2BD1MXot1ektLs%3D
9. JP Levesque-Sergerie M Duquette C Thibault L Delbecchi N Bissonnette 2007 Detection limits of several commercial reverse transcriptase enzymes: Impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR BMC Molecular Biology 22 8 93