E. coli-based cell-free expression, purification and characterization of the membrane-bound ligand-binding CHASE-TM domain of the cytokinin receptor CRE1/AHK4 of arabidopsis thaliana

Molecular Biotechnology

Volumn 47, Issue 3, 2011, Pages 211-219

  Wulfetange, Klaas ^a   Saenger, Wolfram ^a   Schmülling, Thomas ^a   Heyl, Alexander ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Cell free expression; CHASE domain; Cytokinin; Hormone receptor; Membrane protein

Indexed keywords

CELL-FREE EXPRESSION; CHASE DOMAIN; CYTOKININ; HORMONE RECEPTOR; MEMBRANE PROTEIN;

AMINO ACIDS; BIOLOGICAL MEMBRANES; CIRCULAR DICHROISM SPECTROSCOPY; DESORPTION; DICHROISM; ENZYMES; MASS SPECTROMETRY; PURIFICATION; SOAPS (DETERGENTS);

PROTEINS;

CYTOKININ; PROTEIN AHK2; PROTEIN AHK3; PROTEIN AHK4; PROTEIN CHASE; PROTEIN HISTIDINE KINASE; UNCLASSIFIED DRUG; ARABIDOPSIS PROTEIN; CELL SURFACE RECEPTOR; PROTEIN KINASE; WOL PROTEIN, ARABIDOPSIS;

ARABIDOPSIS; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; ESCHERICHIA COLI; LIGAND BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; PROTEIN TERTIARY STRUCTURE;

ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS);

ARABIDOPSIS PROTEINS; CELL-FREE SYSTEM; CIRCULAR DICHROISM; ESCHERICHIA COLI; PROTEIN KINASES; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CELL SURFACE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79955848415     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9331-0     Document Type: Article

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