Medium-throughput SNP genotyping using mass spectrometry: Multiplex snp genotyping using the iplex® gold assay

Methods in Molecular Biology

Volumn 700, Issue , 2011, Pages 61-76

  Millis, Meredith P ^a  

^a TRANSLATIONAL GENOMICS RESEARCH INSTITUTE   (United States)

Author keywords

iPLEX Gold; MALDI TOF MS; Multiplex PCR; Primer extension; SNP genotyping

Indexed keywords

ALKALINE PHOSPHATASE; GENOMIC DNA;

ALLELE; ARTICLE; COMPUTER PROGRAM; GENE AMPLIFICATION; GENOTYPE; MASS SPECTROMETER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM; GENETIC ASSOCIATION; HUMAN; MASS SPECTROMETRY; METHODOLOGY; POLYMERASE CHAIN REACTION;

DECAPODA (CRUSTACEA);

GENOME-WIDE ASSOCIATION STUDY; GENOTYPE; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79955661540     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61737-954-3_5     Document Type: Article

References (4)

1. Ross P, Hall L, Smirnov I, Haff L (1998) High level multiplex genotyping by MALDI-TOF mass spectrometry. Nat Biotechnol 16: 1347-1351
2. Oeth P, Beaulieu L, Park C, Kosman D, Del Mistro G, Van Den Boom D, Jurinke C (2007) iPLEX assay: increased plexing efficiency and flexibility for MassARRAY® system through single base primer extension with mass-modified terminators. Sequenom Application Note. September 27, 2007
3. Haff L, Smirnov I (1997) Single nucleotide polymorphism identification assays using a thermostable DNA polymerase and MALDI-TOF MS. Genome Res 7:378-388
4. de Bakker PIW, Yelensky R, Pe'er I, Gabriel SB, Daly MJ, Altshuler D (2005) Efficiency and power in genetic association studies. Nat Genet 37:1217-1223