Blending of polyethylenimine with a cationic polyurethane greatly enhances both DNA delivery efficacy and reduces the overall cytotoxicity

Current Pharmaceutical Biotechnology

Volumn 12, Issue 5, 2011, Pages 839-846

  Cherng, J Y ^a   Hung, W C ^a   Kao, H C ^a  

^a NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

Author keywords

Blend; Cationic polyurethane; Cytotoxicity; Polyethylenimine; Transfection

Indexed keywords

DNA; NANOPARTICLE; POLYETHYLENEIMINE; POLYURETHAN;

ARTICLE; BIOCOMPATIBILITY; CELL PROLIFERATION; CONTROLLED STUDY; DNA DETERMINATION; DNA TRANSFECTION; DRUG CYTOTOXICITY; GEL ELECTROPHORESIS; GENE TARGETING; GENE THERAPY; PARTICLE SIZE;

EID: 79955629365     PISSN: 13892010     EISSN: 18734316     Source Type: Journal    
DOI: 10.2174/138920111795470985     Document Type: Article

References (25)

1. Moon, C.; Park, J. C.; Chae, Y. K.; Yun, J. H.; Kim, S. Current status of experimental therapeutics for prostate cancer. Cancer Lett., 2008, 266, 116-134.
2. O'Prey, J.; Wilkinson, S.; Ryan, K. M. The tumor antigen LRRC15 impedes adenoviral infection-implications for viral-based cancer therapy. J. Virol., 2008, 10, 2273-2307.
3. Therrien, J. P.; Pfützner, W.; Vogel, J. C. An approach to achieve long-term expression in skin gene therapy. Toxicol Pathol., 2008, 36, 104-111.
4. Hengge, U. R. Gene therapy in mesotheliomas. Onkologie., 2008, 31, 91-96.
5. Wang, J. T.; Peng, D. Y.; Chen, M.; Ye, J. S. Gene delivery for lung cancer using nonviral gene vectors. Pharmazie, 2007, 62, 723-726.
6. Power, A. Wang, T. J.; Falls, T. J.; Paterson, J. M.; Parato, K. A.; Lichty, B. D.; Stojdl, D. F.; Forsyth, P. A.; Atkins, H.; Bell, J. C. Carrier cell-based delivery of an oncolytic virus circumvents antiviral immunity. Mol. Ther., 2007, 15, 123-130.
7. Russ, V.; Elfberg, H.; Thoma, C.; Kloeckner, J.; Ogris, M.; Wagner, E. Novel degradable oligoethylenimine acrylate esterbased pseudodendrimers for in vitro and in vivo gene transfer. Gene Ther., 2008, 15, 18-29.
8. Satoh, T.; Kakimoto, S.; Kano, H.; Nakatani, M.; Shinkai, S.; Nagasaki, T. In vitro gene delivery to HepG2 cells using galactosylated 6-amino-6-deoxychitosan as a DNA carrier. Carbohydr. Res., 2007, 342, 1427-1433.
9. Ito, T.; Iida-Tanaka, N.; Koyama, Y. Efficient in vivo gene transfection by stable DNA/PEI complexes coated by hyaluronic acid. Drug Target., 2008, 16, 276-281.
10. Park, Y. M.; Shin, B. A.; Oh, I. J. Poly(L-lactic acid)/ polyethylenimine nanoparticles as plasmid DNA carriers. Arch. Pharm. Res., 2008, 31, 96-102.
11. Sakae, M.; Ito, T.; Yoshihara, C.; Iida-Tanaka, N.; Yanagie, H.; Eriguchi, M.; Koyama, Y. Highly efficient in vivo gene transfection by plasmid/PEI complexes coated by anionic PEG derivatives bearing carboxyl groups and RGD peptide. Biomed. Pharmacother., 2008, 62, 448-453.
12. Guelcher, S. A.; Srinivasan, A.; Dumas, J. E.; Didier, J. E.; McBride, S.; Hollinger, H. O. Synthesis, mechanical properties, biocompatibility, and biodegradation of polyurethane networks from lysine polyisocyanates. Biomaterials, 2008, 29, 1762-1775.
13. Rockwood, D. N.; Woodhouse, K. A.; Fromstein, J. D.; Chase, D. B.; Rabolt, J. F. Characterization of biodegradable polyurethane microfibers for tissue engineering. J. Biomater. Sci.-Polym. Ed., 2007, 18, 743-758.
14. Shau, M. D.; Tseng, S. J.; Yang, T. F.; Cherng, J. Y.; Chin, W. K. Effect of molecular weight on the transfection efficiency of novel polyurethane as a biodegradable gene vector. J. Biomed. Mater. Res. Part A., 2006, 77, 736-746.
15. Tseng, S. J.; Tang, S. C.; Shau, M. D.; Zeng, Y. F.; Cherng J. Y.; Shih, M. F. Structural characterization and buffering capacity in relation to the transfection efficiency of biodegradable polyurethane. Bioconjugate Chem., 2005, 16, 1375-1381.
16. Yang, T. F.; Chin, W. K.; Cherng, J. Y.; Shau, M. D. Synthesis of novel biodegradable cationic polymer: N, N-Diethylethylenediamine polyurethane as a gene carrier. Biomacromolecules, 2004, 5, 1926-1932.
17. Hung, W. C.; Shau, M. D.; Kao, H. C.; Shih, M. F.; Cherng, J. Y. The synthesis of cationic polyurethanes to study the effect of amines and structures on their DNA transfection potential. J. Control. Release, 2009, 133, 68-76.
18. Funk, D.; Schrenk, H. H.; Frei, E. Serum albumin leads to falsepositive results in the XTT and the MTT assay. Biotechniques, 2007, 43, 178, 180, 182 passim.
19. Scudiero, D. A.; Shoemaker, R. H.; Paull, K. D.; Monks, A.; Tierney, S.; Nofziger, T. H.; Currens, M. J.; Seniff, D.; Boyd, M. R. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 1988, 48, 4827-4833.
20. Luten, J.; van Nostrum, C. F.; DeSmedt, S. C.; Hennink, W. E. Biodegradable polymers as non-viral carriers for plasmid DNA delivery. J. Control. Release, 2008, 126, 97-110.
21. Ahn, C. H.; Chae, S. Y.; Bae, Y. H.; Kim, S. W. Biodegradable poly(ethylenimine) for plasmid DNA delivery. J. Control. Release, 2002, 80, 273-282.
22. Luten, J.; van Steenis, J. H.; van Someren, R.; Kemmink, J.; Schuurmans-Nieuwenbroek, N. M. E.; Koning, G. A.; Crommelin, D. J. A.; van Nostrum, C. F.; Hennink, W. E. Water-soluble biodegradable cationic polyphosphazenes for gene delivery. J. Control. Release, 2003, 89, 483-497.
23. van de Wetering, P.; Cherng, J. Y.; Talsma, H.; Hennink, W. E. Relation between transfection efficiency and cytotoxicity of poly(2-(dimethylamino)ethyl methacrylate)/plasmid complexes. J. Control. Release, 1997, 49, 59-69.
24. Louise, C. Nonviral vectors. Methods Mol. Biol., 2006, 333, 201-226.
25. Vasir, J. K.; Labhasetwar, V. Polymeric nanoparticles for gene delivery. Expert Opin. Drug Deliv., 2006, 3, 325-344.