Production of human β-actin and a mutant using a bacterial expression system with a cold shock vector

Protein Expression and Purification

Volumn 78, Issue 1, 2011, Pages 1-5

  Tamura, Minoru ^a   Ito, Katsunori ^a   Kunihiro, Sachio ^a   Yamasaki, Chihoko ^a   Haragauchi, Mihoko ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Actin; Bacterial expression; Mutation; pCold; Polymerization

Indexed keywords

ACTIN; COLD SHOCK PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MUTATION; WESTERN BLOTTING;

ACTINS; BIOTECHNOLOGY; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; COLD SHOCK PROTEINS AND PEPTIDES; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; HUMANS; MUTATION; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 79955609670     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.09.007     Document Type: Article

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