Generation of targeted chlamydia trachomatis null mutants

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 17, 2011, Pages 7189-7193

  Kari, Laszlo ^a   Goheen, Morgan M ^a   Randall, Linnell B ^a   Taylor, Lacey D ^a   Carlson, John H ^a   Whitmire, William M ^a   Virok, Dezso ^b   Rajaram, Krithika ^c   Endresz, Valeria ^b   McClarty, Grant ^d   Nelson, David E ^c   Caldwell, Harlan D ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b UNIVERSITY OF SZEGED   (Hungary)

^c INDIANA UNIVERSITY   (United States)

^d UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Genetics; Mutation screen

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL MUTATION; BACTERIUM MUTANT; CHLAMYDIA TRACHOMATIS; GENE SEQUENCE; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ANTINEOPLASTIC AGENTS, ALKYLATING; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; ETHYL METHANESULFONATE; HUMANS; MUTAGENESIS; MUTATION; TRYPTOPHAN SYNTHASE;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS; CHLAMYDIAE;

EID: 79955570483     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1102229108     Document Type: Article

References (24)

1. Schachter J (1978) Chlamydial infections (first of three parts). N Engl J Med 298:428-435.
2. Schachter J (1978) Chlamydial infections (third of three parts). N Engl J Med 298:540-549.
3. Schachter J (1978) Chlamydial infections (second of three parts). N Engl J Med 298:490-495.
4. Moulder JW (1982) The relation of basic biology to pathogenic potential in the genus Chlamydia. Infection 10(Suppl 1):S10-S18.
5. Gomes JP, Bruno WJ, Borrego MJ, Dean D (2004) Recombination in the genome of Chlamydia trachomatis involving the polymorphic membrane protein C gene relative to ompA and evidence for horizontal gene transfer. J Bacteriol 186:4295-4306. (Pubitemid 38802578)
6. DeMars R, Weinfurter J, Guex E, Lin J, Potucek Y (2007) Lateral gene transfer in vitro in the intracellular pathogen Chlamydia trachomatis. J Bacteriol 189:991-1003. (Pubitemid 46183874)
7. Suchland RJ, Sandoz KM, Jeffrey BM, Stamm WE, Rockey DD (2009) Horizontal transfer of tetracycline resistance among Chlamydia spp. in vitro. Antimicrob Agents Chemother 53:4604-4611.
8. Binet R, Maurelli AT (2009) Transformation and isolation of allelic exchange mutants of Chlamydia psittaci using recombinant DNA introduced by electroporation. Proc Natl Acad Sci USA 106:292-297.
9. Tam JE, Davis CH, Wyrick PB (1994) Expression of recombinant DNA introduced into Chlamydia trachomatis by electroporation. Can J Microbiol 40:583-591. (Pubitemid 24251554)
10. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18:455-457. (Pubitemid 30217538)
11. Oleykowski CA, Bronson Mullins CR, Godwin AK, Yeung AT (1998) Mutation detection using a novel plant endonuclease. Nucleic Acids Res 26:4597-4602. (Pubitemid 28466605)
12. Ashburner M (1990) Drosophila: A Laboratory Handbook (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY).
13. DeMars R, Weinfurter J (2008) Interstrain gene transfer in Chlamydia trachomatis in vitro: Mechanism and significance. J Bacteriol 190:1605-1614. (Pubitemid 351304016)
14. Dreses-Werringloer U, Padubrin I, Köhler L, Hudson AP (2003) Detection of nucleotide variability in rpoB in both rifampin-sensitive and rifampin-resistant strains of Chlamydia trachomatis. Antimicrob Agents Chemother 47:2316-2318. (Pubitemid 36753591)
15. Kutlin A, Kohlhoff S, Roblin P, Hammerschlag MR, Riska P (2005) Emergence of resistance to rifampin and rifalazil in Chlamydophila pneumoniae and Chlamydia trachomatis. Antimicrob Agents Chemother 49:903-907. (Pubitemid 40327703)
16. Rupp J, Solbach W, Gieffers J (2008) Variation in the mutation frequency determining quinolone resistance in Chlamydia trachomatis serovars L2 and D. J Antimicrob Chemother 61:91-94.
17. Suchland RJ, Bourillon A, Denamur E, Stamm WE, Rothstein DM (2005) Rifampin-resistant RNA polymerase mutants of Chlamydia trachomatis remain susceptible to the ansamycin rifalazil. Antimicrob Agents Chemother 49:1120-1126. (Pubitemid 40327733)
18. Stephens RS, et al. (1998) Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282:754-759. (Pubitemid 28489390)
19. Carlson JH, Porcella SF, McClarty G, Caldwell HD (2005) Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains. Infect Immun 73:6407-6418.
20. Caldwell HD, et al. (2003) Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates. J Clin Invest 111:1757-1769. (Pubitemid 38057756)
21. Fehlner-Gardiner C, et al. (2002) Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase. J Biol Chem 277:26893-26903. (Pubitemid 34951698)
22. Kari L, et al. (2008) Pathogenic diversity among Chlamydia trachomatis ocular strains in nonhuman primates is affected by subtle genomic variations. J Infect Dis 197:449-456. (Pubitemid 351263428)
23. Carlson JH, et al. (2008) The Chlamydia trachomatis plasmid is a transcriptional regulator of chromosomal genes and a virulence factor. Infect Immun 76:2273-2283.
24. Sturdevant GL, et al. (2010) Frameshift mutations in a single novel virulence factor alter the in vivo pathogenicity of Chlamydia trachomatis for the female murine genital tract. Infect Immun 78:3660-3668.