메뉴 건너뛰기




Volumn 90, Issue 4, 2011, Pages 1373-1380

Co-expression of a cellobiose phosphorylase and lactose permease enables intracellular cellobiose utilisation by Saccharomyces cerevisiae

Author keywords

Cellobiose phosphorylase; Cellobiose utilisation; Disaccharide transport; Lactose permease

Indexed keywords

CELLOBIOSE; CELLOBIOSE UTILISATION; CLOSTRIDIUM STERCORARIUM; CO-EXPRESSION; DISACCHARIDE TRANSPORT; HETEROLOGOUS EXPRESSION; KLUYVEROMYCES LACTIS; LACTOSE PERMEASE; OPTIMAL ACTIVITY; PHOSPHOGLYCERATE KINASE; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; SOLE CARBON SOURCE; TRANSCRIPTIONAL CONTROL;

EID: 79955553841     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-011-3164-z     Document Type: Article
Times cited : (58)

References (40)
  • 1
    • 0005299069 scopus 로고
    • Characteristics of cellobiose phosphorylase
    • 1:CAS:528:DyaF3MXhtFyru74%3D
    • JK Alexander 1961 Characteristics of cellobiose phosphorylase J Bacteriol 81 903 910 1:CAS:528:DyaF3MXhtFyru74%3D
    • (1961) J Bacteriol , vol.81 , pp. 903-910
    • Alexander, J.K.1
  • 3
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • 10.1016/0003-2697(76)90527-3 1:CAS:528:DyaE28XksVehtrY%3D
    • MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D
    • (1976) Anal Biochem , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 4
    • 0242391986 scopus 로고    scopus 로고
    • Codon adaptation index as a measure of dominating codon bias
    • DOI 10.1093/bioinformatics/btg272
    • A Carbone A Zinovyev F Kepes 2003 Codon adaptation index as a measure of dominating codon bias Bioinformatics 19 2005 2015 10.1093/bioinformatics/btg272 1:CAS:528:DC%2BD3sXovVelur8%3D (Pubitemid 37408151)
    • (2003) Bioinformatics , vol.19 , Issue.16 , pp. 2005-2015
    • Carbone, A.1    Zinovyev, A.2    Kepes, F.3
  • 5
    • 0024962379 scopus 로고
    • Isolation and characterization of lactose permease mutants with an enhanced recognition of maltose and diminished recognition of cellobiose
    • 1:CAS:528:DyaL1MXlslSqsLs%3D
    • JC Collins SF Permuth RJ Brooker 1989 Isolation and characterization of lactose permease mutants with an enhanced recognition of maltose and diminished recognition of cellobiose J Biol Chem 264 14698 14703 1:CAS:528: DyaL1MXlslSqsLs%3D
    • (1989) J Biol Chem , vol.264 , pp. 14698-14703
    • Collins, J.C.1    Permuth, S.F.2    Brooker, R.J.3
  • 6
    • 0028805858 scopus 로고
    • Cloning and expression of an Aspergillus kawachii endo-1, 4-β-xylanase gene in Saccharomyces cerevisiae
    • 10.1007/BF00310817 1:CAS:528:DyaK2MXpsFChsL8%3D
    • JM Crous IS Pretorius WH van Zyl 1995 Cloning and expression of an Aspergillus kawachii endo-1, 4-β-xylanase gene in Saccharomyces cerevisiae Curr Genet 28 467 473 10.1007/BF00310817 1:CAS:528:DyaK2MXpsFChsL8%3D
    • (1995) Curr Genet , vol.28 , pp. 467-473
    • Crous, J.M.1    Pretorius, I.S.2    Van Zyl, W.H.3
  • 7
    • 33847642855 scopus 로고    scopus 로고
    • Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol
    • DOI 10.1016/j.enzmictec.2006.09.022, PII S0141022906004947
    • R Den Haan JE McBride DC La Grange LR Lynd WH Van Zyl 2007 Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol Enzyme Microb Technol 40 1291 1299 10.1016/j.enzmictec.2006.09.022 (Pubitemid 46356542)
    • (2007) Enzyme and Microbial Technology , vol.40 , Issue.5 , pp. 1291-1299
    • Den Haan, R.1    Mcbride, J.E.2    Grange, D.C.L.3    Lynd, L.R.4    Van Zyl, W.H.5
  • 8
    • 0025802819 scopus 로고
    • Fermentation and aerobic metabolism of cellodextrins by yeasts
    • 1:CAS:528:DyaK3MXhtl2murY%3D
    • SN Freer 1991 Fermentation and aerobic metabolism of cellodextrins by yeasts Appl Environ Microbiol 57 655 659 1:CAS:528:DyaK3MXhtl2murY%3D
    • (1991) Appl Environ Microbiol , vol.57 , pp. 655-659
    • Freer, S.N.1
  • 9
    • 0025269814 scopus 로고
    • Transport of glucose and cellobiose by Candida wickerhamii and Clavispora lusitaniae
    • 1:CAS:528:DyaK3cXltFahs7g%3D
    • SN Freer RV Greene 1990 Transport of glucose and cellobiose by Candida wickerhamii and Clavispora lusitaniae J Biol Chem 265 12864 12868 1:CAS:528:DyaK3cXltFahs7g%3D
    • (1990) J Biol Chem , vol.265 , pp. 12864-12868
    • Freer, S.N.1    Greene, R.V.2
  • 10
  • 11
    • 77957347059 scopus 로고    scopus 로고
    • Cellodextrin transport in yeast for improved biofuel production
    • 10.1126/science.1192838 1:CAS:528:DC%2BC3cXht1WmtbnK
    • JM Galazka C Tian WT Beeson B Martinez NL Glass JH Cate 2010 Cellodextrin transport in yeast for improved biofuel production Science 330 84 86 10.1126/science.1192838 1:CAS:528:DC%2BC3cXht1WmtbnK
    • (2010) Science , vol.330 , pp. 84-86
    • Galazka, J.M.1    Tian, C.2    Beeson, W.T.3    Martinez, B.4    Glass, N.L.5    Cate, J.H.6
  • 12
    • 0021224838 scopus 로고
    • Fermentation of cellodextrins by different yeast strains
    • P Gonde B Blondin M Leclerc R Ratomahenina A Arnaud P Galzy 1984 Fermentation of cellodextrins by different yeast strains Appl Environ Microbiol 48 265 269 1:CAS:528:DyaL2cXltVyqsbw%3D (Pubitemid 14068413)
    • (1984) Applied and Environmental Microbiology , vol.48 , Issue.2 , pp. 265-269
    • Gonde, P.1    Blondin, B.2    Leclerc, M.3
  • 13
    • 3042782585 scopus 로고    scopus 로고
    • Codon bias and heterologous protein expression
    • DOI 10.1016/j.tibtech.2004.04.006, PII S0167779904001118
    • CS Gustafsson S Govindarajan J Minshull 2004 Codon bias and heterologous protein expression Trends Biotechnol 22 346 353 10.1016/j.tibtech.2004.04.006 1:CAS:528:DC%2BD2cXls1Wktbc%3D (Pubitemid 38887543)
    • (2004) Trends in Biotechnology , vol.22 , Issue.7 , pp. 346-353
    • Gustafsson, C.1    Govindarajan, S.2    Minshull, J.3
  • 14
    • 79551670374 scopus 로고    scopus 로고
    • Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
    • 10.1073/pnas.1010456108 1:CAS:528:DC%2BC3MXptFKntg%3D%3D
    • SJ Ha JM Galazka S Rin Kim JH Choi X Yang JH Seo NL Glass JH Cate YS Jin 2011 Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation Proc Natl Acad Sci USA 108 504 509 10.1073/pnas.1010456108 1:CAS:528:DC%2BC3MXptFKntg%3D%3D
    • (2011) Proc Natl Acad Sci USA , vol.108 , pp. 504-509
    • Ha, S.J.1    Galazka, J.M.2    Rin Kim, S.3    Choi, J.H.4    Yang, X.5    Seo, J.H.6    Glass, N.L.7    Cate, J.H.8    Jin, Y.S.9
  • 15
    • 77957219655 scopus 로고
    • DMSO-enhanced whole cell yeast transformation
    • 10.1093/nar/19.20.5791 1:CAS:528:DyaK3MXmslalsbo%3D
    • J Hill KA Donald DE Griffiths G Donald 1991 DMSO-enhanced whole cell yeast transformation Nucleic Acids Res 19 5791 10.1093/nar/19.20.5791 1:CAS:528:DyaK3MXmslalsbo%3D
    • (1991) Nucleic Acids Res , vol.19 , pp. 5791
    • Hill, J.1    Donald, K.A.2    Griffiths, D.E.3    Donald, G.4
  • 17
    • 0027214236 scopus 로고
    • Triggering of cellulase biosynthesis by cellulose in Trichoderma reesei. Involvement of a constitutive, sophorose-inducible, glucose-inhibited β- diglucoside permease
    • CP Kubicek R Messner F Gruber M Mandels EM Kubicek-Pranz 1993 Triggering of cellulase biosynthesis by cellulose in Trichoderma reesei. Involvement of a constitutive, sophorose-inducible, glucose-inhibited β-diglucoside permease J Biol Chem 268 19364 19368 1:CAS:528:DyaK3sXltlGht7c%3D (Pubitemid 23270712)
    • (1993) Journal of Biological Chemistry , vol.268 , Issue.26 , pp. 19364-19368
    • Kubicek, C.P.1    Messner, R.2    Gruber, F.3    Mandels, M.4    Kubicek-Pranz, E.M.5
  • 18
    • 0030051146 scopus 로고    scopus 로고
    • Expression of a Trichoderma reesei β-xylanase gene (XYN2) in Saccharomyces cerevisiae
    • DC la Grange IS Pretorius WH van Zyl 1996 Expression of a Trichoderma reesei β-xylanase gene (XYN2) in Saccharomyces cerevisiae Appl Environ Microbiol 62 1036 1044
    • (1996) Appl Environ Microbiol , vol.62 , pp. 1036-1044
    • La Grange, D.C.1    Pretorius, I.S.2    Van Zyl, W.H.3
  • 19
    • 0033199721 scopus 로고    scopus 로고
    • Biocommodity engineering
    • DOI 10.1021/bp990109e
    • LR Lynd CE Wyman TU Gerngross 1999 Biocommodity engineering Biotechnol Prog 15 777 793 10.1021/bp990109e 1:CAS:528:DyaK1MXlvVGiu7Y%3D (Pubitemid 29475617)
    • (1999) Biotechnology Progress , vol.15 , Issue.5 , pp. 777-793
    • Lynd, L.R.1    Wyman, C.E.2    Gerngross, T.U.3
  • 20
    • 0036714783 scopus 로고    scopus 로고
    • Microbial cellulose utilization: Fundamentals and biotechnology
    • DOI 10.1128/MMBR.66.3.506-577.2002
    • LR Lynd PJ Weimer WH van Zyl IS Pretorius 2002 Microbial cellulose utilization: fundamentals and biotechnology Microbiol Mol Biol Rev 66 506 577 10.1128/MMBR.66.3.506-577.2002 1:CAS:528:DC%2BD38XnsFOitrk%3D (Pubitemid 35005827)
    • (2002) Microbiology and Molecular Biology Reviews , vol.66 , Issue.3 , pp. 506-577
    • Lynd, L.R.1    Weimer, P.J.2    Van Zyl, W.H.3    Pretorius, I.S.4
  • 21
    • 25844505728 scopus 로고    scopus 로고
    • Consolidated bioprocessing of cellulosic biomass: An update
    • DOI 10.1016/j.copbio.2005.08.009, PII S0958166905001369, Tissue and Cell Engineering/Biochemical Engineering
    • LR Lynd WH van Zyl JE McBride M Laser 2005 Consolidated bioprocessing of cellulosic biomass: an update Curr Opin Biotechnol 16 577 583 10.1016/j.copbio.2005.08.009 1:CAS:528:DC%2BD2MXhtVKjsLnE (Pubitemid 41393839)
    • (2005) Current Opinion in Biotechnology , vol.16 , Issue.5 , pp. 577-583
    • Lynd, L.R.1    Van Zyl, W.H.2    McBride, J.E.3    Laser, M.4
  • 22
    • 18144391576 scopus 로고    scopus 로고
    • Utilization of cellobiose by recombinant β-glucosidase-expressing strains of Saccharomyces cerevisiae: Characterization and evaluation of the sufficiency of expression
    • 10.1016/j.enzmictec.2005.01.034 1:CAS:528:DC%2BD2MXjvVKqs7c%3D
    • JE McBride JJ Zietsman WH van Zyl LR Lynd 2005 Utilization of cellobiose by recombinant β-glucosidase-expressing strains of Saccharomyces cerevisiae: characterization and evaluation of the sufficiency of expression Enzyme Microb Technol 37 93 101 10.1016/j.enzmictec.2005.01.034 1:CAS:528:DC%2BD2MXjvVKqs7c%3D
    • (2005) Enzyme Microb Technol , vol.37 , pp. 93-101
    • McBride, J.E.1    Zietsman, J.J.2    Van Zyl, W.H.3    Lynd, L.R.4
  • 23
    • 0042201948 scopus 로고    scopus 로고
    • Characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, Thermotoga maritima MSB8
    • E Rajashekhara M Kitaoka YK Kim K Hayashi 2002 Characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, Thermotoga maritima MSB8 Biosci Biotechnol Biochem 66 2578 2586 10.1271/bbb.66.2578 1:CAS:528:DC%2BD3sXht1Gqsw%3D%3D (Pubitemid 39251128)
    • (2002) Bioscience, Biotechnology and Biochemistry , vol.66 , Issue.12 , pp. 2578-2586
    • Rajashekhara, E.1    Kitaoka, M.2    Kim, Y.-K.3    Hayashi, K.4
  • 24
    • 0037742423 scopus 로고    scopus 로고
    • Kinetics of β-glucosidase production by Saccharomyces cerevisiae recombinants harboring heterologous bgl genes
    • DOI 10.1023/A:1024042401544
    • MI Rajoka F Shaukat MT Ghauri R Shahid 2003 Kinetics of β-glucosidase production by Saccharomyces cerevisiae recombinants harboring heterologous bgl genes Biotechnol Lett 25 945 948 10.1023/A:1024042401544 1:CAS:528:DC%2BD3sXktlagu78%3D (Pubitemid 36796912)
    • (2003) Biotechnology Letters , vol.25 , Issue.12 , pp. 945-948
    • Rajoka, M.I.1    Shaukat, F.2    Ghauri, M.T.3    Shahid, R.4
  • 25
    • 0030609809 scopus 로고    scopus 로고
    • Purification and properties of a celiobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium
    • M Reichenbecher F Lottspeich K Bronnenmeier 1997 Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium Eur J Biochem 247 262 267 10.1111/j.1432-1033.1997.00262.x 1:CAS:528:DyaK2sXksFGhsro%3D (Pubitemid 27319512)
    • (1997) European Journal of Biochemistry , vol.247 , Issue.1 , pp. 262-267
    • Reichenbecher, M.1    Lottspeich, F.2    Bronnenmeier, K.3
  • 26
    • 0025669949 scopus 로고
    • The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus
    • 10.1016/0923-2508(90)90079-6 1:CAS:528:DyaK3MXksVGjt7o%3D
    • J Reizer A Reizer MH Saier Jr 1990 The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus Res Microbiol 141 1061 1067 10.1016/0923-2508(90)90079-6 1:CAS:528:DyaK3MXksVGjt7o%3D
    • (1990) Res Microbiol , vol.141 , pp. 1061-1067
    • Reizer, J.1    Reizer, A.2    Saier, Jr.M.H.3
  • 28
    • 0029800765 scopus 로고    scopus 로고
    • Expression and secretion of the candida wickerhamii extracellular β-glucosidase gene, bglB, in saccharomyces cerevisiae
    • DOI 10.1007/s002940050151
    • CD Skory SN Freer RJ Bothast 1996 Expression and secretion of the Candida wickerhamii extracellular β-glucosidase gene, bglB, in Saccharomyces cerevisiae Curr Genet 30 417 422 10.1007/s002940050151 1:CAS:528: DyaK28XnsVWquro%3D (Pubitemid 26402480)
    • (1996) Current Genetics , vol.30 , Issue.5 , pp. 417-422
    • Skory, C.D.1    Freer, S.N.2    Bothast, R.J.3
  • 30
    • 0034093058 scopus 로고    scopus 로고
    • Kinetics of active sucrose transport in Saccharomyces cerevisiae
    • DOI 10.1016/S1389-1723(00)88742-3
    • BU Stambuk AS Batista PS de Araujo 2000 Kinetics of active sucrose transport in Saccharomyces cerevisiae J Biosci Bioeng 89 212 214 10.1016/S1389-1723(00)88742-3 1:CAS:528:DC%2BD3cXisVaht74%3D (Pubitemid 30195910)
    • (2000) Journal of Bioscience and Bioengineering , vol.89 , Issue.2 , pp. 212-214
    • Stambuk, B.U.1    Batista, A.S.2    De Araujo, P.S.3
  • 31
    • 0036159062 scopus 로고    scopus 로고
    • Hydrolysis of lignocellulosic materials for ethanol production: A review
    • DOI 10.1016/S0960-8524(01)00212-7, PII S0960852401002127
    • Y Sun J Cheng 2002 Hydrolysis of lignocellulosic materials for ethanol production: a review Bioresour Technol 83 1 11 10.1016/S0960-8524(01)00212-7 1:CAS:528:DC%2BD38Xptleluw%3D%3D (Pubitemid 34126398)
    • (2002) Bioresource Technology , vol.83 , Issue.1 , pp. 1-11
    • Sun, Y.1    Cheng, J.2
  • 32
    • 0028931975 scopus 로고
    • Purification and properties of cellobiose phosphorylase from Clostridium thermocellum
    • 10.1016/0922-338X(95)90605-Y
    • K Tanaka T Kawaguchi Y Imada T Ooi M Arai 1994 Purification and properties of cellobiose phosphorylase from Clostridium thermocellum J Ferment Bioeng 79 212 216 10.1016/0922-338X(95)90605-Y
    • (1994) J Ferment Bioeng , vol.79 , pp. 212-216
    • Tanaka, K.1    Kawaguchi, T.2    Imada, Y.3    Ooi, T.4    Arai, M.5
  • 33
    • 0020787415 scopus 로고
    • An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels
    • 10.1016/0003-2697(83)90419-0 1:CAS:528:DyaL3sXktlGlt70%3D
    • D Tautz M Renz 1983 An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels Anal Biochem 132 14 19 10.1016/0003-2697(83) 90419-0 1:CAS:528:DyaL3sXktlGlt70%3D
    • (1983) Anal Biochem , vol.132 , pp. 14-19
    • Tautz, D.1    Renz, M.2
  • 34
    • 0015132619 scopus 로고
    • A comparison of β-glucanase and β-glucosidase in Saccharomyces lactis
    • 1:CAS:528:DyaE38XlsFyhsQ%3D%3D
    • M Tingle HO Halvorson 1971 A comparison of β-glucanase and β-glucosidase in Saccharomyces lactis Biochim Biophys Acta 250 165 171 1:CAS:528:DyaE38XlsFyhsQ%3D%3D
    • (1971) Biochim Biophys Acta , vol.250 , pp. 165-171
    • Tingle, M.1    Halvorson, H.O.2
  • 35
    • 0015319389 scopus 로고
    • Biochemical and genetic characterization of 3 β-glucosidase mutants in Saccharomyces lactis
    • 1:CAS:528:DyaE38XhsFSrtbk%3D
    • M Tingle HO Halvorson 1972 Biochemical and genetic characterization of 3 β-glucosidase mutants in Saccharomyces lactis J Bacteriol 110 196 201 1:CAS:528:DyaE38XhsFSrtbk%3D
    • (1972) J Bacteriol , vol.110 , pp. 196-201
    • Tingle, M.1    Halvorson, H.O.2
  • 37
    • 0032518904 scopus 로고    scopus 로고
    • Engineering yeast for efficient cellulose degradation
    • DOI 10.1002/(SICI)1097-0061(19980115)14:1<67::AID-YEA200>3.0.CO;2-T
    • P van Rensburg WH van Zyl IS Pretorius 1998 Engineering yeast for efficient cellulose degradation Yeast 14 67 76 10.1002/(SICI)1097-0061(19980115) 14:1<67::AID-YEA200>3.0.CO;2-T (Pubitemid 28035344)
    • (1998) Yeast , vol.14 , Issue.1 , pp. 67-76
    • Van Rensburg, P.1    Van Zyl, W.H.2    Pretorius, I.S.3
  • 38
    • 27544459042 scopus 로고    scopus 로고
    • Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains
    • DOI 10.1016/j.jbiotec.2005.06.013, PII S0168165605003287
    • R van Rooyen B Hahn-Hägerdal DC la Grange WH van Zyl 2005 Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains J Biotechnol 120 284 295 10.1016/j.jbiotec.2005.06.013 (Pubitemid 41540385)
    • (2005) Journal of Biotechnology , vol.120 , Issue.3 , pp. 284-295
    • Van Rooyen, R.1    Hahn-Hagerdal, B.2    La Grange, D.C.3    Van Zyl, W.H.4
  • 39
    • 0033810985 scopus 로고    scopus 로고
    • Cloning and characterization of the glucooligosaccharide catabolic pathway β-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana
    • 10.1128/JB.182.18.5172-5179.2000 1:CAS:528:DC%2BD3cXmsVOqurY%3D
    • DA Yernool JK McCarthy DE Eveleigh JD Bok 2000 Cloning and characterization of the glucooligosaccharide catabolic pathway β-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana J Bacteriol 182 5172 5179 10.1128/JB.182.18.5172-5179. 2000 1:CAS:528:DC%2BD3cXmsVOqurY%3D
    • (2000) J Bacteriol , vol.182 , pp. 5172-5179
    • Yernool, D.A.1    McCarthy, J.K.2    Eveleigh, D.E.3    Bok, J.D.4
  • 40
    • 1642273858 scopus 로고    scopus 로고
    • Kinetics and Relative Importance of Phosphorolytic and Hydrolytic Cleavage of Cellodextrins and Cellobiose in Cell Extracts of Clostridium thermocellum
    • DOI 10.1128/AEM.70.3.1563-1569.2004
    • YH Zhang LR Lynd 2004 Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum Appl Environ Microbiol 70 1563 1569 10.1128/AEM.70.3.1563-1569.2004 1:CAS:528:DC%2BD2cXisVKisro%3D (Pubitemid 38365139)
    • (2004) Applied and Environmental Microbiology , vol.70 , Issue.3 , pp. 1563-1569
    • Zhang, Y.-H.P.1    Lynd, L.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.