Multiple-locus variable-number tandem-repeat analysis is a suitable tool for differentiation of Mycoplasma hyopneumoniae strains without cultivation

Journal of Clinical Microbiology

Volumn 49, Issue 5, 2011, Pages 2020-2023

  Vranckx, K ^a   Maes, D ^a   Calus, D ^a   Villarreal, I ^a   Pasmans, F ^a   Haesebrouck, F ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; GENE LOCUS; GENE SEQUENCE; HERD; LUNG LAVAGE; MYCOPLASMA HYOPNEUMONIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STRAIN DIFFERENCE; SWINE; THROAT CULTURE; VALIDATION PROCESS; VARIABLE NUMBER OF TANDEM REPEAT;

ANIMALS; BACTERIAL TYPING TECHNIQUES; BRONCHOALVEOLAR LAVAGE FLUID; CLUSTER ANALYSIS; DNA, BACTERIAL; GENOTYPE; MINISATELLITE REPEATS; MOLECULAR SEQUENCE DATA; MOLECULAR TYPING; MYCOPLASMA HYOPNEUMONIAE; PNEUMONIA OF SWINE, MYCOPLASMAL; POLYMORPHISM, GENETIC; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TRACHEA;

MYCOPLASMA HYOPNEUMONIAE; SUIDAE; SUS;

EID: 79955549902     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00125-11     Document Type: Article

References (14)

1. Calus, D., et al. 2007. Protein variability among Mycoplasma hyopneumoniae isolates. Vet. Microbiol. 120:284-291.
2. Frey, J., A. Haldimann, and J. Nicolet. 1992. Chromosomal heterogeneity of various Mycoplasma hyopneumoniae field strains. Int. J. Syst. Bacteriol. 42:275-280.
3. Friis, N. F. 1975. Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare: a survey. Nord. Vet. Med. 27:337-339.
4. Hannan, P. C., B. S. Bhogal, and J. P. Fish. 1982. Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses. Res. Vet. Sci. 33:76-88.
5. Hunter, P. R., and M. A. Gaston. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26:2465-2466.
6. Mayor, D., J. Jores, B. M. Korczak, and P. Kuhnert. 2008. Multilocus sequence typing (MLST) of Mycoplasma hyopneumoniae: a diverse pathogen with limited clonality. Vet. Microbiol. 127:63-72.
7. Mayor, D., F. Zeeh, J. Frey, and P. Kuhnert. 2007. Diversity of Mycoplasma hyopneumoniae in pig farms revealed by direct molecular typing of clinical material. Vet. Res. 38:391-398.
8. Minion, F. C., et al. 2004. The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis. J. Bacteriol. 186:7123-7133. (Pubitemid 39383096)
9. Stakenborg, T., et al. 2006. A multiplex PCR to identify porcine mycoplasmas present in broth cultures. Vet. Res. Commun. 30:239-247.
10. Stakenborg, T., et al. 2005. The diversity of Mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis. Vet. Microbiol. 109:29-36.
11. Stakenborg, T., et al. 2006. Comparison of molecular techniques for the typing of Mycoplasma hyopneumoniae isolates. J. Microbiol. Methods 66:263-275. (Pubitemid 43903032)
12. Vicca, J., et al. 2003. Evaluation of virulence of Mycoplasma hyopneumoniae field isolates. Vet. Microbiol. 97:177-190.
13. Villarreal, I., et al. 2009. Infection with a low virulent Mycoplasma hyopneumoniae isolate does not protect piglets against subsequent infection with a highly virulent M. hyopneumoniae isolate. Vaccine 27:1875-1879.
14. Villarreal, I., et al. 2010. The effect of vaccination on the transmission of Mycoplasma hyopneumoniae in pigs under field conditions. Vet. J. 188:48-52.