Chimeric porcine circoviruses (PCV) containing amino acid epitope tags in the C terminus of the capsid gene are infectious and elicit both anti-epitope tag antibodies and anti-PCV type 2 neutralizing antibodies in pigs

Journal of Virology

Volumn 85, Issue 9, 2011, Pages 4591-4595

  Beach, Nathan M ^a   Smith, Sara M ^a   Ramamoorthy, Sheela ^a,b   Meng, Xiang Jin ^a  

^a VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CAPSID PROTEIN; CIRCOVIRUS TYPE 2 ANTIBODY; CIRCOVIRUS VACCINE; EPITOPE; EPITOPE ANTIBODY; MODIFIED LIVE ATTENUATED VACCINE; NEUTRALIZING ANTIBODY; UNCLASSIFIED DRUG; VIRUS VACCINE;

ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ARTICLE; CAPSID GENE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CIRCOVIRIDAE INFECTION; CIRCOVIRUS; GENE LOCATION; GENOMICS; HUMAN; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; VACCINE PRODUCTION; VIRUS CAPSID; VIRUS GENE; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS NEUTRALIZATION; VIRUS STRAIN;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIBODIES, VIRAL; CAPSID PROTEINS; CIRCOVIRUS; EPITOPES; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SWINE; VACCINES, ATTENUATED; VACCINES, MARKER; VIRAL VACCINES;

MIRIDAE; PORCINE CIRCOVIRUS; SUIDAE; SUS;

EID: 79955403919     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.02294-10     Document Type: Article

References (28)

1. Allan, G. M., et al. 2004. PMWS: experimental model and co-infections. Vet. Microbiol. 98:165-168.
2. Allan, G. M., et al. 1998. Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the U. S. A. and Europe. J. Vet. Diagn. Invest. 10:3-10.
3. Beach, N. M., N. M. Juhan, L. Cordoba, and X. J. Meng. 2010. Replacement of the replication factors of porcine circovirus (PCV) type 2 with those of PCV type 1 greatly enhances viral replication in vitro. J. Virol. 84:8986-8989.
4. Beach, N. M., S. Ramamoorthy, T. Opriessnig, S. Q. Wu, and X. J. Meng. 2010. Novel chimeric porcine circovirus (PCV) with the capsid gene of the emerging PCV2b subtype cloned in the genomic backbone of the non-pathogenic PCV1 is attenuated in vivo and induces protective and cross-protective immunity against PCV2b and PCV2a subtypes in pigs. Vaccine 29:221-232.
5. Cheung, A. K. 2003. The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2. Virology 313:452-459.
6. Cheung, A. K. 2003. Transcriptional analysis of porcine circovirus type 2. Virology 305:168-180.
7. Ellis, J., et al. 1998. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can. Vet. J. 39:44-51.
8. Fenaux, M., et al. 2002. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J. Virol. 76:541-551.
9. Fenaux, M., T. Opriessnig, P. G. Halbur, F. Elvinger, and X. J. Meng. 2004. A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J. Virol. 78:6297-6303.
10. Fenaux, M., T. Opriessnig, P. G. Halbur, and X. J. Meng. 2003. Immuno-genicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. J. Virol. 77:11232-11243.
11. Finsterbusch, T., and A. Mankertz. 2009. Porcine circoviruses-small but powerful. Virus Res. 143:177-183.
12. Gedvilaite, A., et al. 2000. Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein. Virology 273:21-35.
13. Gillespie, J., et al. 2008. A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo. Vaccine 26:4231-4236.
14. Grau-Roma, L., L. Fraile, and J. Segales. 2011. Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2. Vet. J. 187:23-32.
15. Lekcharoensuk, P., et al. 2004. Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. J. Virol. 78:8135-8145.
16. Mankertz, A., and B. Hillenbrand. 2001. Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene. Virology 279: 429-438.
17. Meehan, B. M., et al. 1998. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J. Gen. Virol. 79(Pt. 9):2171-2179.
18. Murray, K., and A. L. Shiau. 1999. The core antigen of hepatitis B virus as a carrier for immunogenic peptides. Biol. Chem. 380:277-283.
19. Nawagitgul, P., et al. 2002. Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin. Diagn. Lab. Immunol. 9:33-40.
20. Nawagitgul, P., et al. 2000. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J. Gen. Virol. 81:2281-2287.
21. Neugebauer, M., et al. 2006. Development of a vaccine marker technology: display of B cell epitopes on the surface of recombinant polyomavirus-like pentamers and capsoids induces peptide-specific antibodies in piglets after vaccination. Biotechnol. J. 1:1435-1446.
22. Niikura, M., et al. 2002. Chimeric recombinant hepatitis E virus-like particles as an oral vaccine vehicle presenting foreign epitopes. Virology 293:273-280.
23. Segalés, J., et al. 2009. A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms. Vaccine 27:7313-7321.
24. Shang, S. B., et al. 2009. Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus type 2. Mol. Immunol. 46:327-334.
25. Slupetzky, K., et al. 2001. Chimeric papillomavirus-like particles expressing a foreign epitope on capsid surface loops. J. Gen. Virol. 82:2799-2804.
26. Tischer, I., H. Gelderblom, W. Vettermann, and M. A. Koch. 1982. A very small porcine virus with circular single-stranded DNA. Nature 295:64-66.
27. Tischer, I., W. Mields, D. Wolff, M. Vagt, and W. Griem. 1986. Studies on epidemiology and pathogenicity of porcine circovirus. Arch. Virol. 91:271-276.
28. Todd, D., et al. 2005. Circoviridae, p. 327-334. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball (ed.), Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego, CA.