Automated immunomagnetic processing and separation of Legionella pneumophila with manual detection by sandwich ELISA and PCR amplification of the ompS gene

Journal of Laboratory Automation

Volumn 16, Issue 2, 2011, Pages 157-164

  Reidt, Ulrich ^a   Geisberger, Beatrice ^a   Heller, Christoph ^a   Friedberger, Alois ^a  

^a AIRBUS GROUP INNOVATIONS   (Germany)

Author keywords

Automated processing; Immunomagnetic separation; Legionella pneumophila; ompS; Sandwich ELISA

Indexed keywords

ASSAYS; AUTOMATION; GENES; MICROORGANISMS; POLYMERASE CHAIN REACTION; SEPARATION;

AUTOMATED PROCESSING; IMMUNOMAGNETIC SEPARATION; LEGIONELLA PNEUMOPHILA; OMPS; SANDWICH ELISA;

BACTERIA;

OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN S; UNCLASSIFIED DRUG;

ARTICLE; AUTOANALYSIS; BACTERIAL COUNT; BACTERIAL GENOME; BACTERIAL VIRULENCE; BACTERIUM DETECTION; CONTROLLED STUDY; DNA SEQUENCE; ENRICHMENT CULTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE TARGETING; GENETIC CONSERVATION; IMMUNOMAGNETIC SEPARATION; LABORATORY AUTOMATION; LEGIONELLA PNEUMOPHILA; LIQUID CULTURE; MARKER GENE; NONHUMAN; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; SENSITIVITY ANALYSIS; SPECIES DIFFERENCE;

EID: 79955038908     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1016/j.jala.2010.07.003     Document Type: Article

References (18)

1. Brenner, D. J.; Steigerwalt, A. G.; McDade, J. E. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann. Intern. Med. 1979, 90(4), 656-658.
2. Blackmon, J. A.; Chandler, F. W.; Cherry, W. B.; England, A. C. 3rd; Feeley,J.C.; Hicklin,M.D.; McKinney, R.M.; Wilkinson, H.W.Legionellosis. Am. J. Pathol. 1981, 103(3), 429-465.
3. Jonas, D.; Rosenbaum, A.; Weyrich, S.; Bhakdi, S. Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bron-choalveolar fluid. J. Clin. Microbiol. 1995, 33(5), 1247-1252.
4. Stolhaug, A.; Bergh, K. Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing. Appl. Environ. Microbiol. 2006, 72(9), 6394-6398.
5. Yanez, M. A.; Carrasco-Serrano, C.; Barbera, V. M.; Catalan, V. Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene. Appl. Environ. Microbiol. 2005, 71(7), 3433-3441.
6. Baudart, J.; Coallier, J.; Laurent, P.; Prevost, M. Rapid and sensitive enumeration of viable diluted cells of members of the family enterobacteriaceae in freshwater and drinking water. Appl. Environ. Microbiol. 2002, 68(10), 5057-5063.
7. Declerck, P.; Verelst, L.; Duvivier, L.; Van Damme, A.; Ollevier, F. A detection method for Legionella spp in (cooling) water: fluorescent in situ hybridisation (FISH) on whole bacteria. Water Sci. Technol. 2003, 47(3), 143-146.
8. Delgado-Viscogliosi, P.; Simonart, T.; Parent, V.; Marchand, G.; Dobbe-laere, M.; Pierlot, E.; Pierzo, V.; Menard-Szczebara, F.; Gaudard-Ferveur, E.; Delabre, K.; Delattre, J. M. Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water. Appl. Environ. Microbiol. 2005, 71(7), 4086-4096.
9. Reidt, U.; Chauhan, L.; Muller, G.; Molz, R.; Lindner, P.; Wolf, H.; Friedberger, A. Reproducible filtration of bacteria with micromechanical filters. J. Rapid Methods Autom. Microbiol. 2008, 16(4), 337-350.
10. Lund, A.; Hellemann, A. L.; Vartdal, F. Rapid isolation of K88 + Escherichia coli by using immunomagnetic particles. J Clin Microbiol 1988, 26(12), 2572-2575.
11. Stark, M.; Reizenstein, E.; Uhlen, M.; Lundeberg, J. Immunomagnetic separation and solid-phase detection of Bordetella pertussis. J. Clin. Microbiol. 1996, 34(4), 778-784.
12. Olsvik, O.; Popovic, T.; Skjerve, E.; Cudjoe, K. S.; Hornes, E.; Ugelstad, J.; Uhlen, M. Magnetic separation techniques in diagnostic microbiology. Clin. Microbiol. Rev. 1994, 7(1), 43-54.
13. Roberts, B.; Hirst, R. Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans. J. Clin. Microbiol. 1997, 35(10), 2709-2711.
14. Inokuchi, H. N.; Suzuki, Y.; Kasagi, N.; Shikazono, N. In Evaluation of Lamination Micro Mixer for Micro Immunomagnetic Cell Sorter, Microtechnologies in Medicine and Biology; 9-12 May 2006; pp 131-134 Okinawa, Japan.
15. Mullane, N. R.; Murray, J.; Drudy, D.; Prentice, N.; Whyte, P.; Wall, P. G.; Parton, A.; Fanning, S. Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture. Appl. Environ. Microbiol. 2006, 72(9), 6325-6330.
16. Hoffman, P. S.; Ripley, M.; Weeratna, R. Cloning and nucleotide sequence of a gene (ompS) encoding the major outer membrane protein of Legionella pneumophila. J. Bacteriol. 1992, 174(3), 914-920.
17. Hoffman, P. S.; Seyer, J. H.; Butler, C. A. Molecular characterization of the 28- and 31-kilodalton subunits of the Legionella pneumophila major outer membrane protein. J. Bacteriol. 1992, 174(3), 908-913.
18. Burnette, W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 1981, 112(2), 195-203.