Automated extraction of DNA from blood and PCR setup using a tecan freedom EVO liquid handler for forensic genetic STR typing of reference samples

Journal of Laboratory Automation

Volumn 16, Issue 2, 2011, Pages 134-140

  Stangegaard, Michael ^a   Frøslev, Tobias G ^a   Frank Hansen, Rune ^a   Hansen, Anders J ^a   Morling, Niels ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Forensic genetics; Laboratory automation; Magnetic beads; Nucleic acid extraction; Reference samples

Indexed keywords

AUTOMATION; BLOOD; CARRIER CONCENTRATION; DNA; FORENSIC SCIENCE; LIQUIDS; MAGNETIC SEPARATION; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION;

ACID EXTRACTION; FORENSIC GENETICS; LABORATORY AUTOMATION; MAGNETIC BEADS; REFERENCE SAMPLES;

EXTRACTION;

ARTICLE; AUTOANALYZER; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA EXTRACTION; DNA FINGERPRINTING; DNA PURIFICATION; FORENSIC GENETICS; INSTRUMENT VALIDATION; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; MEDICAL INSTRUMENTATION; MICROTITER PLATE ASSAY; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; QUALITY CONTROL; ROBOTICS; SEPARATION TECHNIQUE; SHORT TANDEM REPEAT;

EID: 79955000781     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1016/j.jala.2010.11.003     Document Type: Article

References (15)

1. Anslinger, K.; Bayer, B.; Rolf, B.; Keil, W.; Eisenmenger, W. Application of the BioRobot EZ1 in a forensic laboratory. Leg. Med. (Tokyo) 2005, 7(3), 164-168.
2. Valgren, C.; Wester, S.; Hansson, O. A comparison of three automated DNA purification methods in Forensic casework. Forensic Sci. Int. Gene. Suppl. 2. 2008, 1, 76-77.
3. Rockenbauer, E.; Børsting, C.; Stangegaard, M.; Frank-Hansen, R.; Morling, N. Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot EZ1 Workstation. Forensic Sci. Int. Gene. Suppl. 2. 2009, 2, 83-84.
4. Stangegaard, M.; Jørgensen, M.; Hansen, A. J.; Morling, N. Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. Forensic Sci. Int. Gene. Suppl. 2. 2009, 2, 69-70.
5. Krnajski, Z.; Geering, S.; Steadman, S. Performance verification of the Maxwell 16 Instrument and DNA IQ Reference Sample Kit for automated DNA extraction of known reference samples. Forensic Sci. Med. Pathol. 2007, 3(4), 264-269.
6. Nagy, M.; Otremba, P.; Kruger, C; Bergner-Greiner, S.; Anders, P.; Henske, B.; Prinz, M.; Roewer, L. Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics. Forensic Sci. Int. 2005, 152(1), 13-22.
7. Gehrig, C; Kummer, D.; Castella, V. Automated DNA extraction using the QIAsymphony platform: estimation of DNA recovery from simulated forensic stains. Forensic Sci. Int. Gene. Suppl. 2. 2009, 2 (1), 85-86.
8. Fregeau, C. J.; Lett, C. M.; Elliott, J.; Yensen, C; Fourney, R. M. Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: contamination prevention. J. Forensic Sci. 2008, 53(3), 632-651.
9. Fregeau, C. J.; Yensen, C; Elliott, J.; Fourney, R. M. Optimized configuration of fixed-tip robotic liquid-handling stations for the elimination of biological sample cross-contamination. J. Assoc. Lab. Autom. 2007, 12(6), 339-354.
10. Walsh, P. S.; Metzger, D. A.; Higuchi, R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 1991, 10(4), 506-513.
11. Stangegaard, M.; Frøslev, T. G; Frank-Hansen, R.; Lauersen, S. S.; Jørgensen, M.; Hansen, A. J.; Morling, N. Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler. Forensic Sci. Int. Gene. Suppl. 2. 2009, 2, 74-76.
12. Witlox, K. J.; Nguyen, T. N; Bruggink, L. D.; Catton, M. G; Marshall, J. A. A comparative evaluation of the sensitivity of two automated and two manual nucleic acid extraction methods for the detection of norovi-rus by RT-PCR. J. Virol. Methods 2008, 150(1-2), 70-72.
13. Riemann, K; Adamzik, M.; Frauenrath, S.; Egensperger, R.; Schmid, K. W.; Brockmeyer, N. H.; Siffert, W. Comparison of manual and automated nucleic acid extraction from whole-blood samples. J. Clin. Lab. Anal. 2007, 21(4), 244-248.
14. Stangegaard, M.; Hansen, A. J.; Frøslev, T. G; Morling, N. A simple method for validation and verification of pipettes mounted on automated liquid handlers. J. Assoc. Lab. Autom. 2010, in press.
15. Björkstén, M. G; Almby, B.; Jansson, E. S. Hand and shoulder ailments among laboratory technicians using modern plunger-operated pipettes. Appl. Ergon. 1994, 25(2), 88-94.