Quantitative comparison of different purification and detection methods for Cryptosporidium parvum oocysts

Veterinary Parasitology

Volumn 177, Issue 3-4, 2011, Pages 366-370

  Kar, Sirri ^a   Gawlowska, Sandra ^b   Daugschies, Arwid ^b   Bangoura, Berit ^b  

^a NAMIK KEMAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Carbol fuchsin; Cryptosporidium parvum; Efficiency; Flotation methods; Modified Ziehl Neelsen staining; PCR

Indexed keywords

SODIUM CHLORIDE;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; FECES ANALYSIS; FLOTATION; INTERMETHOD COMPARISON; MICROORGANISM DETECTION; NONHUMAN; OOCYST; PARASITE IDENTIFICATION; PARASITE ISOLATION; POLYMERASE CHAIN REACTION; PURIFICATION; QUANTITATIVE ANALYSIS; STAINING; SUCROSE DENSITY GRADIENT CENTRIFUGATION;

ANIMALS; CATTLE; CATTLE DISEASES; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; GASTROINTESTINAL DISEASES; HISTOCYTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; OOCYSTS; POLYMERASE CHAIN REACTION; ROSANILINE DYES; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BOS; CRYPTOSPORIDIUM PARVUM;

EID: 79954605211     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.12.005     Document Type: Article

References (13)

1. Arrowood M.J., Sterling C.R. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic percoll gradients. J. Parasitol. 1987, 73(2):314-319.
2. Bukhari Z., Smith H.V. Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J. Clin. Microbiol. 1995, 33(10):2592-2595.
3. Cacciò S., Homan W., van Dijk K., Pozio E. Genetic polymorphism at the β-tubulin locus among human and animal isolates of Cryptosporidium parvum. FEMS Microbiol. Lett. 1999, 170(1):173-179.
4. Casemore D.P. Laboratory methods for diagnosing cryptosporidiosis. J. Clin. Pathol. 1991, 44(6):445-451.
5. Casemore D.P., Armstrong M., Sands R.L. Laboratory diagnosis of cryptosporidiosis. J. Clin. Pathol. 1985, 38:1337-1341.
6. Current W.L. Techniques and laboratory maintenance of Cryptosporidium. Cryptosporidiosis of Man and Animals 1990, 32-49. CRC Press, USA, Boca Raton. J.P. Dubey, C.A. Speer, R. Fayer (Eds.).
7. Gasser R.B., OrDonoghue P. Isolation, propagation and characterisation of Cryptosporidium. Int. J. Parasitol. 1999, 29:1379-1413.
8. Heine J. Eine einfache Nachweismethode fur Kryptosporidien im Kot. Zbl. Vet. Med. B. 1982, 29:324-327.
9. Henriksen S.A., Pohlenz J.F. Staining of Cryptosporidia by a modified Ziehl-Neelsen technique. Acta Vet. Scand. 1981, 22(3-4):594-596.
10. Kar S., Daugschies A., Bangoura B. Comparative efficacy of conventional primer sets in detection of Cryptosporidium parvum for diagnostic use. Parasitol. Res. 2010, 106(3):683-687.
11. Peeters J.E., Van Opdenbosch E., Glorieux B. Demonstration of cryptosporidia in calf faeces: a comparative study. Vlaams Diergeneeskd. Tijdschr. 1982, 51:513-523.
12. Peeters J., Villacorta I. Cryptosporidium. Biotechnology: Guidelines on Techniques in Coccidiosis Research. European Commission 1995, ECSC-EC-EAEC, Brussels, Belgium, pp. 202-240. R. Braun, M.W. Shirley, P. Coudert (Eds.).
13. Young P.L., Komisar S.J. Impacts of viability and purification on the specific gravity of Cryptosporidium oocysts. Water Res. 2005, 39(14):3349-3359.