Reverse genetics plasmid for cloning unstable Influenza A virus gene segments

Journal of Virological Methods

Volumn 173, Issue 2, 2011, Pages 378-383

  Zhou, Bin ^a,b   Jerzak, Greta ^a   Scholes, Derek T ^a   Donnelly, Matthew E ^a   Li, Yan ^a   Wentworth, David E ^a,b  

^a WADSWORTH CENTER   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

Influenza; Ligation independent cloning; Low copy plasmid; Recombination based cloning; Reverse genetics; Unstable gene

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; INFLUENZA VIRUS A; MOLECULAR CLONING; NONHUMAN; PB2 GENE; PLASMID; PRIORITY JOURNAL; REVERSE GENETICS; VIRAL GENETICS; VIRUS GENE; VIRUS RECOMBINANT; VIRUS REPLICATION;

ANIMALS; CLONING, MOLECULAR; GENE DOSAGE; GENETIC ENGINEERING; GENETIC VECTORS; GENETICS, MICROBIAL; GENOMIC INSTABILITY; HUMANS; INFLUENZA A VIRUS; MOLECULAR BIOLOGY; PLASMIDS; VIROLOGY;

ANIMALIA; AVES; INFLUENZA A VIRUS; ORTHOMYXOVIRIDAE;

EID: 79954604723     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.01.021     Document Type: Article

References (9)

1. Fodor E., Devenish L., Engelhardt O.G., Palese P., Brownlee G.G., Garcia-Sastre A. Rescue of influenza A virus from recombinant DNA. J. Virol. 1999, 73:9679-9682.
2. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., Webster R.G. A DNA transfection system for generation of influenza A virus from eight plasmids. Proc. Natl. Acad. Sci. U.S.A. 2000, 97:6108-6113.
3. Lopilato J., Bortner S., Beckwith J. Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol. Gen. Genet. 1986, 205:285-290.
4. Neumann G., Watanabe T., Ito H., Watanabe S., Goto H., Gao P., Hughes M., Perez D.R., Donis R., Hoffmann E., Hobom G., Kawaoka Y. Generation of influenza A viruses entirely from cloned cDNAs. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:9345-9350.
5. Palese P., Shaw M.L. Orthomyxoviridae: the viruses and their replication. Fields Virology 2007, 1647-1690. Lippincott Williams and Wilkins, Philadelphia, PA. D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, M.A. Martin, B. Roizman, S.E. Straus (Eds.).
6. Wright P.F., Neumann G., Kawaoka Y. Orthomyxoviruses. Fields Virology 2007, 1691-1740. Lippincott Williams and Wilkins, Philadelphia, PA. D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, M.A. Martin, B. Roizman, S.E. Straus (Eds.).
7. Zhou B., Donnelly M.E., Scholes D.T., George K., Hatta M., Kawaoka Y., Wentworth D.E. Single-reaction genomic amplification accelerates sequencing and vaccine production for classical and swine origin human influenza A viruses. J. Virol. 2009, 83:10309-10313.
8. Zhou B., Li Y., Belser J.A., Pearce M.B., Schmolke M., Subba A.X., Shi Z., Zaki S.R., Blau D.M., Garcia-Sastre A., Tumpey T.M., Wentworth D.E. NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets. Vaccine 2010, 28:8015-8125.
9. Zhou B., Li Y., Halpin R., Hine E., Spiro D.J., Wentworth D.E. PB2 residue 158 is a pathogenic determinant of pandemic-H1N1 and H5 Influenza A viruses in mice. J. Virol. 2011, 85:357-365.