Genomic fingerprinting and serotyping of Salmonella from Galápagos iguanas demonstrates island differences in strain diversity

Environmental Microbiology Reports

Volumn 3, Issue 2, 2011, Pages 166-173

  Wheeler, Emily ^a   Cann, Isaac K O ^a   Mackie, Roderick I ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMBLYRHYNCHUS CRISTATUS; BACTERIA (MICROORGANISMS); CONOLOPHUS SUBCRISTATUS; IGUANA; IGUANIA; REPTILIA; SALMONELLA; SALMONELLA ENTERICA; SQUAMATA;

EID: 79954574128     PISSN: None     EISSN: 17582229     Source Type: Journal    
DOI: 10.1111/j.1758-2229.2010.00203.x     Document Type: Article

References (36)

1. Alcaine, S.D., Soyer, Y., Warnick, L.D., Su, W.L., Sukhnanand, S., Richards, J., etal. (2006) Multilocus sequence typing supports the hypothesis that cow- and human-associated Salmonella isolates represent distinct and overlapping populations. Appl Environ Microbiol 72: 7575-7585.
2. Bauwens, L., Vercammen, F., Bertrand, S., Collard, J.-M., and De Ceuster, S. (2006) Isolation of Salmonella from environmental samples collected in the reptile department of Antwerp Zoo using different selective methods. J Appl Microbiol 101: 284-289.
3. Bemis, D.A., Grupka, L.M., Liamthong, S., Folland, D.W., Sykes, J.M., and Ramsay, E.C. (2007) Clonal relatedness of Salmonella isolates associated with invasive infectionsin captive and wild-caught rattlesnakes. Vet Microbiol 120: 300-307.
4. Beyer, W., Mukendi, F.M., Kimmig, P., and Bohm, R. (1998) Suitability of repetitive-DNA-sequence-based PCR fingerprinting for characterizing epidemic isolates of Salmonella enterica serovar Saintpaul. J Clin Microbiol 36: 1549-1554.
5. Briones, V., Téllez, S., Goyache, J., Ballesteros, C., Lanzarot, M.D., and Dominguez, L. (2004) Salmonella diversity associated with wild reptiles and amphibians in Spain. Environ Microbiol 6: 868-871.
6. Burdick, J.R., Lyngby, L.K., and Tarr, R.S. (1984) Isolation of Salmonella from laboratory-housed iguanid lizards. J Herpetol 18: 88-91.
7. Christian, K.A., Tracy, C.R., and Porter, W.P. (1984) Diet, digestion, and food preferences of Galápagos land iguanas. Herpetologica 40: 205-212.
8. Corrente, M., Madio, A., Friedrich, K.G., Greco, G., Desario, C., Tagliabue, M., etal. (2004) Isolation of Salmonella strains from reptile faeces and comparison of different culture media. J Appl Microbiol 96: 709-715.
9. Edwards, R.A., Olsen, G.J., and Maloy, S.R. (2002) Comparative genomics of closely related salmonellae. Trends Microbiol 10: 94-99.
10. Fakhr, M.K., Nolan, L.K., and Logue, C.M. (2005) Multilocus sequence typing lacks the discriminatory ability of pulse-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium. J Clin Microbiol 43: 2215-2219.
11. Greenberg, Z., and Sechter, I. (1992) Salmonella serotypes isolated from snakes and other reptiles-Israel 1953-1989. Isr J Vet Med 47: 49-60.
12. Groisman, E.A., and Ochman, H. (1997) How Salmonella became a pathogen. Trends Microbiol 5: 343-349.
13. Grupka, L.M., Ramsay, E.C., and Bemis, D.A. (2006) Salmonella surveillance in a collection of rattlesnakes (Crotalus spp.). J Zoo Wildl Med 37: 306-312.
14. Guan, T.Y., and Holley, R.A. (2003) Pathogen survival in swine manure environments and transmission of human enteric illness - a review. J Environ Qual 32: 383-392.
15. Harbottle, H., White, D.G., McDermott, P.F., Walker, R.D., and Zhao, S. (2006) Comparison of multilocus sequence typing, pulse-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates. J Clin Microbiol 44: 2449-2457.
16. Hudson, C.R., Garcia, M., Gast, R.K., and Maurer, J.J. (2001) Determination of close genetic relatedness of the major Salmonella enteritidis phage types by pulse-field gel electrophoresis and DNA sequence analysis of several Salmonella virulence genes. Avian Dis 45: 875-886.
17. Ishii, S., and Sadowsky, M.J. (2009) Application of the rep-PCR DNA fingerprinting technique to study microbial diversity, ecology and evolution. Environ Microbiol 11: 733-740.
18. Le Minor, L. (1992) Taxonomie et nomenclature des Salmonella. Med Mal Infect 22: 246-248.
19. Legendre, P., and Legendre, L. (1998) Numerical Ecology, 2nd edn. Amsterdam, the Netherlands: Elsevier.
20. Mitchell, M.A., and Shane, S.M. (2000) Preliminary findings of Salmonella spp. in captive green iguanas (Iguana iguana) and their environment. Prev Vet Med 45: 297-304.
21. Morgan, E. (2007) Salmonella pathogenicity islands. In Salmonella Molecular Biology and Pathogenesis. Rhen, M., Maskell, D., Mastroeni, P., and Threlfall, J. (eds). Trowbridge, UK: Cromwell Press, pp. 67-88.
22. Oksanen, J., Kindt, R., Legendre, P., and O'Hara, B. (2008) vegan: Community Ecology Package. R package version 1.15-0 [WWW document]. URL, .
23. Popoff, M.Y., and Le Minor, L. (1997) Antigenic Formulas of the Salmonella Serovars (7th Revision). Paris, France: WHO Collaborating Center for Reference and Research on Salmonella Institute Pasteur.
24. Rademaker, J.L.W., Louws, F.J., and De Bruijn, F.J. (1998) Characterization of the diversity of ecologically important microbes by rep-PCR genomic fingerprinting. In Molecular Microbial Ecology Manual. Akkermans, A.D.L., van Elsas, J.D., and de Bruijn, F.J. (eds). Dordrecht, the Netherlands: Kluwer Academic Publishers, pp. 1-27.
25. Rassmann, K., Tautz, D., Trillmich, F., and Gliddon, C. (1997a) The microevolution of the Galápagos marine iguana Amblyrhynchus cristatus assessed by nuclear and mitochondrial genetic analyses. Mol Ecol 6: 437-452.
26. Rassmann, K., Trillmich, F., and Tautz, D. (1997b) Hybridization between the Galápagos land and marine iguana (Conolophus subcristatus and Amblyrhynchus cristatus) on Plaza Sur. J Zool 242: 729-739.
27. Rassmann, K., Markmann, M., Trillmich, F., and Tautz, D. (2004) Tracing the evolution of the Galápagos iguanas. In Iguanas, Biology and Conservation. Alberts, A.C., Carter, R.L., Hayes, W.K., and Martins, E. (eds). Berkeley, CA, USA: University of California Press, pp. 71-83.
28. Rozas, J., Sánchez-DelBarrio, J.C., Messeguer, X., and Rozas, R. (2003) DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics 19: 2496-2497.
29. Snell, H.L., Snell, H.M., and Tracy, C.R. (1984) Variation among populations of Galápagos land iguanas (Conolophus): contrasts of phylogeny and ecology. Biol J Linn Soc 21: 185-208.
30. Sukhnanand, S., Alcaine, S., Warnick, L.D., etal. (2005) DNA sequence-based subtyping and evolutionary analysis of selected Salmonella enterica serotypes. J Clin Microbiol 43: 3688-3698.
31. Thaller, M.C., Ciambotta, M., Sapochetti, M., Migliore, L., Tapia, W., Cdedfio, V., and Gentle, G. (2010a) Uneven frequency of Vibrio alginnolyticus-group isolates among different populations of Galápagos marine iguanas (Amblyrhynchus cristatus). Environ Microbiol Rep 2: 179-184.
32. Thaller, M.C., Migliore, L., Marquez, C., Tapia, W., Cedeño, V., Rossolini, G.M., and Gentile, G. (2010b) Tracking acquired antibiotic resistance in commensal bacteria of Galápagos land iguanas: no man, no resistance. PLoS ONE 5: e8989.
33. Trillmich, K.G.K., and Trillmich, F. (1986) Foraging strategies of the marine iguana, Amblyrhynchus cristatus. Behav Ecol Sociobiol 18: 259-266.
34. Versalovic, J., Koeuth, T., and Lupski, J.R. (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19: 6823-6831.
35. Weigel, R.M., Qiao, B., Teferedegne, B., Suh, D.K., Barber, D.A., Isaacson, R.E., and White, B.A. (2004) Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reactions as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella. Vet Microbiol 100: 205-217.
36. Weigel, R.M., Nucera, D., Qiao, B.Z., Teferedegne, B., Suh, D.K., Barber, D.A., etal. (2007) Testing an ecological model for transmission of Salmonella enterica in swine production ecosystems using genotyping data. Prev Vet Med 81: 274-289.