A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea

Journal of Virological Methods

Volumn 173, Issue 2, 2011, Pages 390-393

  Khamrin, Pattara ^a   Okame, Makiko ^b   Thongprachum, Aksara ^b   Nantachit, Nattika ^c   Nishimura, Shuichi ^b   Okitsu, Shoko ^b   Maneekarn, Niwat ^a   Ushijima, Hiroshi ^b  

^a Faculty of Medicine Chiang Mai University   (Thailand)

^b NIHON UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c Mahidol University   (Thailand)

Author keywords

Diarrhea; Multiplex PCR; Stool; Virus

Indexed keywords

ACUTE GASTROENTERITIS; ADENOVIRUS; ARTICLE; ASTROVIRUS; CHILD; CONTROLLED STUDY; DIARRHEA; ENTEROVIRUS; FECES ANALYSIS; HUMAN; INFANT; JAPAN; KOBUVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NOROVIRUS; PARECHOVIRUS; PRESCHOOL CHILD; PRIORITY JOURNAL; ROTAVIRUS; SAPOVIRUS; VIRUS DETECTION; VIRUS IDENTIFICATION;

CHILD, PRESCHOOL; DIARRHEA; FECES; GASTROENTERITIS; HUMANS; INFANT; INFANT, NEWBORN; JAPAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIROLOGY; VIRUS DISEASES; VIRUSES;

ADENOVIRIDAE; AICHI VIRUS; ASTROVIRIDAE; ENTEROVIRUS; NOROVIRUS; PARECHOVIRUS; ROTAVIRUS; ROTAVIRUS A; ROTAVIRUS C; SAPOVIRUS;

EID: 79954573776     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.02.012     Document Type: Article

References (14)

1. Chow B.D., Ou Z., Esper F.P. Newly recognized bocaviruses (HBoV HBoV2) in children and adults with gastrointestinal illness in the United States. J. Clin. Virol. 2010, 47:143-147.
2. Clark B., McKendrick M. A review of viral gastroenteritis. Curr. Opin. Infect. Dis. 2004, 17:461-469.
3. Joki-Korpela P., Hyypia T. Diagnosis and epidemiology of echovirus 22 infections. Clin. Infect. Dis. 1998, 27:129-136.
4. Pham N.T., Khamrin P., Nguyen T.A., Kanti D.S., Phan T.G., Okitsu S., Ushijima H. Isolation and molecular characterization of Aichi viruses from fecal specimens collected in Japan, Bangladesh Thailand, and Vietnam. J. Clin. Microbiol. 2007, 45:2287-2288.
5. Pham N.T., Trinh Q.D., Chan-It W., Khamrin P., Shimizu H., Okitsu S., Mizuguchi M., Ushijima H. A novel RT-multiplex PCR for detection of Aichi virus, human parechovirus, enteroviruses, and human bocavirus among infants and children with acute gastroenteritis. J. Virol. Methods 2010, 169:193-197.
6. Phan T.G., Nguyen T.A., Shimizu H., Yagyu F., Okitsu S., Müller W.E., Ushijima H. Identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in Ho Chi Minh City Vietnam. J. Med. Virol. 2005, 77:257-264.
7. Reuter G., Boldizsár A., Papp G., Pankovics P. Detection of Aichi virus shedding in a child with enteric and extraintestinal symptoms in Hungary. Arch. Virol. 2009, 154:1529-1532.
8. Stanway G., Joki-Korpela P., Hyypiä T. Human parechoviruses-biology and clinical significance. Rev. Med. Virol. 2000, 10:57-69.
9. Svraka S., Rosario K., Duizer E., van der Avoort H., Breitbart M., Koopmans M.P. Metagenomic sequencing for virus identification in a public health setting. J. Gen. Virol. 2010, 91:2846-2856.
10. Tang P., Chiu C. Metagenomics for the discovery of novel human viruses. Future Microbiol. 2010, 5:177-189.
11. Yamashita T., Sugiyama M., Tsuzuki H., Sakae K., Suzuki Y., Miyazaki Y. Application of a reverse transcription-PCR for identification and differentiation of Aichi virus, a new member of the Picornavirus family associated with gastroenteritis in humans. J. Clin. Microbiol. 2000, 38:2955-2961.
12. Yan H., Nguyen T.A., Phan T.G., Okitsu S., Li Y., Ushijima H. Development of RT-multiplex PCR assay for detection of adenovirus and group A and C rotaviruses in diarrheal fecal specimens from children in China. Kansenshogaku Zasshi 2004, 78:699-709.
13. Yan H., Yagyu F., Okitsu S., Nishio O., Ushijima H. Detection of norovirus (GI, GII) Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR. J. Virol. Methods 2003, 114:37-44.
14. Zoll G.J., Melchers W.J., Kopecka H., Jambroes G., van der Poel H.J., Galama J.M. General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. J. Clin. Microbiol. 1992, 30:160-165.