Semi-automated image analysis: Detecting carbonylation in subcellular regions of skeletal muscle

Analytical and Bioanalytical Chemistry

Volumn 400, Issue 1, 2011, Pages 213-222

  Kostal, Vratislav ^a   Levar, Kiara ^a   Swift, Mark ^a   Skillrud, Erik ^a   Chapman, Mark ^a   Thompson, Ladora V ^a   Arriaga, Edgar A ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Aging; Carbonylation; Fluorescence microscopy; Image analysis; ImageJ; MatLab; Mitochondria; Muscle fiber

Indexed keywords

[CARBONYL; AGING; DINITROPHENYLHYDRAZINE; EXTRACELLULAR REGION; IMAGEJ; IMAGING ANALYSIS; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOLABELING; MUSCLE FIBER; OXIDATIVE DAMAGE; PHYSICAL PERFORMANCE; SECONDARY ANTIBODIES; SEMI-AUTOMATED; SKELETAL MUSCLE; SUB-CELLULAR;

ANTIBODIES; FIBERS; FLUORESCENCE; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; MITOCHONDRIA; MUSCLE;

CARBONYLATION;

2,4 DINITROPHENYLHYDRAZINE; 2,4-DINITROPHENYLHYDRAZINE; PHENYLHYDRAZINE DERIVATIVE;

ANIMAL; ARTICLE; AUTOMATION; CELL FRACTIONATION; CHEMISTRY; FISCHER 344 RAT; FLUORESCENCE MICROSCOPY; RAT; SKELETAL MUSCLE;

ANIMALS; AUTOMATION; MICROSCOPY, FLUORESCENCE; MUSCLE, SKELETAL; PHENYLHYDRAZINES; RATS; RATS, INBRED F344; SUBCELLULAR FRACTIONS;

EID: 79954447862     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-011-4725-9     Document Type: Article

References (20)

1. A Federico L Manneschi E Paolini 1987 Biochemical difference between intermyofibrillar and subsarcolemmal mitochondria from human-muscle J Inherit Metab Dis 10 242 246 10.1007/BF01811416
2. DA Hood 2001 Plasticity in skeletal, cardiac, and smooth muscle-invited review: contractile activity-induced mitochondrial biogenesis in skeletal muscle J Appl Physiol 90 3 1137 1157 1:CAS:528:DC%2BD3MXjsVWmu7s%3D (Pubitemid 32171405)
3. JW Palmer B Tandler CL Hoppel 1977 Biochemical properties of subsarcolemmal and inter-fibrillar mitochondria isolated from rat cardiac-muscle J Biol Chem 252 23 8731 8739 1:CAS:528:DyaE1cXhvFyh (Pubitemid 8243644)
4. S Judge YM Jang A Smith C Selman T Phillips JR Speakman T Hagen C Leeuwenburgh 2005 Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart Am J Physiol Reg I 289 6 R1564 R1572 1:CAS:528:DC%2BD28XlslGqug%3D%3D
5. ER Stadtman BS Berlett 1997 Reactive oxygen-mediated protein oxidation in aging and disease Chem Res Toxicol 10 5 485 494 10.1021/tx960133r 1:CAS:528:DyaK2sXjs1WgtbY%3D (Pubitemid 27217204)
6. J Bautista MD Mateos-Nevado 1998 Immunological detection and quantification of oxidized proteins by labelling with digoxigenin Biosci Biotechnol Biochem 62 3 419 423 10.1271/bbb.62.419 1:CAS:528:DyaK1cXisFymtLs%3D (Pubitemid 128474940)
7. ER Stadtman 2006 Protein oxidation and aging Free Radic Res 40 12 1250 1258 10.1080/10715760600918142 1:CAS:528:DC%2BD28XhtlGqs7jF (Pubitemid 44698274)
8. ER Stadtman 2001 Protein oxidation in aging and age-related diseases Ann NY Acad Sci 928 22 38 10.1111/j.1749-6632.2001.tb05632.x 1:STN:280: DC%2BD38%2FntVSjuw%3D%3D (Pubitemid 32386064)
9. J Feng EA Arriaga 2008 Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection Electrophoresis 29 2 475 482 10.1002/elps.200700262 1:CAS:528:DC%2BD1cXhvVOmur4%3D (Pubitemid 351201524)
10. P Wang SR Powell 2010 Decreased sensitivity associated with an altered formulation of a commercially available kit for detection of protein carbonyls Free Radic Biol Med 49 2 119 121 10.1016/j.freeradbiomed.2010.03.005 1:CAS:528:DC%2BC3cXntVOntrs%3D
11. S Luo NB Wehr 2009 Protein carbonylation: avoiding pitfalls in the 2, 4-dinitrophenylhydrazine assay Redox Rep 14 4 159 166 10.1179/135100009X392601 1:CAS:528:DC%2BD1MXhsFKrs7bN
12. J Feng HW Xie DL Meany LV Thompson EA Arriaga TJ Griffin 2008 Quantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondria J Gerontol A Biol 63 11 1137 1152 (Pubitemid 352772034)
13. DL Meany HW Xie LV Thompson EA Arriaga TJ Griffin 2007 Identification of carbonylated proteins from enriched rat skeletal muscle mitochondria using affinity chromatography-stable isotope labeling and tandem mass spectrometry Proteomics 7 7 1150 1163 10.1002/pmic.200600450 1:CAS:528:DC%2BD2sXksFSlu74%3D (Pubitemid 46649164)
14. J Feng M Navratil LV Thompson EA Arriaga 2008 Estimating relative carbonyl levels in muscle microstructures by fluorescence imaging Anal Bioanal Chem 391 7 2591 2598 10.1007/s00216-008-2187-5 1:CAS:528:DC%2BD1cXptFSqtLc%3D
15. R Development Core Team (2010) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at http://www.R-project.org
16. Portney LG, Watkins MP (1993) Foundations of clinical research applications to practice. Appleton & Lange, Stamford
17. JM Fagan M Ganguly H Stockman LH Ferland M Toner 1999 Posttranslational modifications of cardiac and skeletal muscle proteins by reactive oxygen species after burn injury in the rat Ann Surg 229 1 106 114 10.1097/00000658-199901000- 00014 1:STN:280:DyaK1M7itVKrsA%3D%3D (Pubitemid 30190387)
18. JM Fagan BG Sleczka I Sohar 1999 Quantitation of oxidative damage to tissue proteins Int J Biochem Cell Biol 31 7 751 757 10.1016/S1357-2725(99) 00034-5 1:CAS:528:DyaK1MXltlKrur8%3D (Pubitemid 29333119)
19. E Barreiro B de la Puente S Busquets FJ Lopez-Soriano J Gea JM Argiles 2005 Both oxidative and nitrosative stress are associated with muscle wasting in tumour-bearing rats FEBS Lett 579 7 1646 1652 10.1016/j.febslet.2005.02.017 1:CAS:528:DC%2BD2MXitFChsrg%3D (Pubitemid 40341537)
20. MS Lustgarten YC Jang YH Liu FL Muller WB Qi M Steinhelper SV Brooks L Larkin T Shimizu T Shirasawa LM McManus A Bhattacharya A Richardson H Van Remmen 2009 Conditional knockout of Mn-SOD targeted to type IIB skeletal muscle fibers increases oxidative stress and is sufficient to alter aerobic exercise capacity Am J Physiol Cell Ph 297 6 C1520 C1532 10.1152/ajpcell.00372.2009 1:CAS:528:DC%2BD1MXhsFOgsbvK