Probing the active site of cellodextrin phosphorylase from Clostridium stercorarium: Kinetic characterization, ligand docking, and site-directed mutagenesis

Biotechnology Progress

Volumn 27, Issue 2, 2011, Pages 326-332

  Hai Tran, Giang ^a   Desmet, Tom ^a   De Groeve, Manu R M ^a   Soetaert, Wim ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Cellodextrin phosphorylase; Homology modeling; Ligand docking; Substrate specificity

Indexed keywords

ACTIVE SITE; BINDING AFFINITIES; CATALYTIC ACIDS; CATALYTIC ACTIVITY; CELLODEXTRIN; CLOSTRIDIUM STERCORARIUM; ENZYME-SUBSTRATE INTERACTIONS; GLYCOSYLATED; HOMOLOGY MODELING; KINETIC CHARACTERIZATION; LIGAND DOCKING; PURIFIED ENZYME; SITE DIRECTED MUTAGENESIS; STACKING INTERACTION; SUBSITES; SUBSTRATE SPECIFICITY;

BINDING ENERGY; CARBOHYDRATES; CATALYST ACTIVITY; CLOSTRIDIUM; ENZYMES; ESCHERICHIA COLI; LIGANDS; MUTAGENESIS; OXYGEN; PHOSPHORYLATION; SUBSTRATES;

ENZYME ACTIVITY;

CELLODEXTRIN PHOSPHORYLASE; GLUCOSYLTRANSFERASE; LIGAND;

ARTICLE; CHEMISTRY; CLOSTRIDIUM; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; GLYCOSYLATION; KINETICS; METABOLISM; MOLECULAR CLONING; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

CATALYTIC DOMAIN; CLONING, MOLECULAR; CLOSTRIDIUM; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; GLYCOSYLATION; KINETICS; LIGANDS; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

CLOSTRIDIUM; CLOSTRIDIUM STERCORARIUM; ESCHERICHIA COLI;

EID: 79954422270     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.555     Document Type: Article

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