Evaluation of the Gen-Probe Aptima HIV-1 RNA qualitative assay as an alternative to Western blot analysis for confirmation of HIV infection

Journal of Clinical Microbiology

Volumn 49, Issue 4, 2011, Pages 1642-1645

  Pierce, Virginia M ^a   Neide, Brandy ^a   Hodinka, Richard L ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIRETROVIRUS AGENT; VIRUS RNA;

ALGORITHM; ARTICLE; BLOOD SAMPLING; ENZYME IMMUNOASSAY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1 RNA QUALITATIVE ASSAY; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOASSAY; INTERMETHOD COMPARISON; NUCLEIC ACID AMPLIFICATION; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RISK FACTOR; SENSITIVITY AND SPECIFICITY; VIRUS LOAD; WESTERN BLOTTING;

ADOLESCENT; ADULT; BLOTTING, WESTERN; CHILD; CHILD, PRESCHOOL; HIV INFECTIONS; HUMANS; INFANT; INFANT, NEWBORN; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; YOUNG ADULT;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 79953874289     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02183-10     Document Type: Article

References (16)

1. Bennett, B., et al. 2009. HIV testing algorithms: A status report. Association of Public Health Laboratories, Silver Spring, MD. http://www.aphl.org/hiv /statusreport.
2. Branson, B. M. 2007. State of the art for diagnosis of HIV infection. Clin. Infect. Dis. 45:S221-S225. (Pubitemid 351417220)
3. Branson, B., et al. 2006. Revised recommendations for HIV testing of adults, adolescents, and pregnant women in health-care settings. MMWR Recommend. Rep. 55(RR-14):1-17.
4. CDC. 2008. Revised surveillance case definitions for HIV infection among adults, adolescents, and children aged <18 months and for HIV infection and AIDS among children aged 18 months to <13 years-United States, 2008. MMWR Recommend. Rep. 57(RR-10):1-12.
5. CDC. 1989. Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type 1 infections. MMWR Morb. Mortal. Wkly. Rep. 38(Suppl. 7):1-7.
6. CDC. 1987. Perspectives in disease prevention and health promotion: Public health service guidelines for counseling and antibody testing to prevent HIV infection and AIDS. MMWR Morb. Mortal. Wkly. Rep. 36:509-515.
7. Chantry, C. J., et al. 1995. Seroreversion in human immunodeficiency virusexposed but uninfected infants. Pediatr. Infect. Dis. J. 14:382-387.
8. Ethridge, S. F., et al. 2010. Performance of the Aptima HIV-1 RNA qualitative assay with 16- and 32-member specimen pools. J. Clin. Microbiol. 48:3343-3345.
9. Gen-Probe, Inc. 2009. Aptima HIV-1 RNA qualitative assay product insert. Gen-Probe, Inc., San Diego, CA. http://www.gen-probe.com/pdfs /pi/501623RevA.pdf.
10. Giachetti, C., et al. 2002. Highly sensitive multiplex assay for detection of human immunodeficiency virus type 1 and hepatitis C virus RNA. J. Clin. Microbiol. 40:2408-2419. (Pubitemid 34747340)
11. Kerr, R. J., G. Player, S. A. Fiscus, and J. A. E. Nelson. 2009. Qualitative human immunodeficiency virus RNA analysis of dried blood spots for diagnosis of infections in infants. J. Clin. Microbiol. 47:220-222.
12. Maxim Biomedical, Inc. 2009. Cambridge Biotech HIV-1 Western blot kit product insert. Maxim Biomedical, Inc., Rockville, MD. http://www.mbidiagnostic. com/images/HIV-1-SERUM-WESTERN-BLOT-KIT-PN98002-PACKAGE-INSERT-REV-06b.pdf.
13. Owen, S. M., et al. 2008. Alternative algorithms for human immunodeficiency virus infection diagnosis using tests that are licensed in the United States. J. Clin. Microbiol. 46:1588-1595. (Pubitemid 351657199)
14. Read, J. S., and the Committee on Pediatric AIDS. 2007. Diagnosis of HIV-1 infection in children younger than 18 months in the United States. Pediatrics 120:e1547-e1562.
15. Ren, A., et al. 2008. Screening and confirmation of human immunodeficiency virus type 1 infection solely by detection of RNA. J. Med. Microbiol. 57: 1228-1233.
16. Stevens, W. S., L. Noble, L. Berrie, S. Sarang, and L. E. Scott. 2009. Ultrahigh- throughput, automated nucleic acid detection of human immunodeficiency virus (HIV) for infant infection diagnosis using the Gen-Probe Aptima HIV-1 screening assay. J. Clin. Microbiol. 47:2465-2469.