Chromosome Analysis and Sorting Using Flow Cytometry

Methods in Molecular Biology

Volumn 701, Issue , 2011, Pages 221-238

  Doležel, Jaroslav ^a   Kubaláková, Marie ^a   íhalíková, Jarmila ^a   Suchánková, Pavla ^a   Šimková, Hana ^a  

^a INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

Author keywords

Accumulation of metaphases; Cell cycle synchrony; Cytogenetics stocks; Flow karyotype; Fluorescence in situ hybridization; Hydroponics; Mitotic synchrony; Plant chromosome isolation; Plant flow cytogenetics; Root tip meristem

Indexed keywords

PLANT DNA;

ARTICLE; CELL CYCLE; CYTOGENETICS; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; KARYOTYPING; MERISTEM; METHODOLOGY; PLANT; PLANT CHROMOSOME; PLANT SEED;

CELL CYCLE; CHROMOSOMES, PLANT; CYTOGENETICS; DNA, PLANT; FLOW CYTOMETRY; IN SITU HYBRIDIZATION, FLUORESCENCE; KARYOTYPING; MERISTEM; PLANTS; SEEDS;

MLCS; MLOWN;

EID: 79953775556     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-957-4_12     Document Type: Chapter

References (29)

1. Gray, J.W., Carrano, A.V., Steinmetz, L.L., Van Dilla, M.A., Moore, H.H., Mayall, B.H., and Mendelsohn, M.L. (1975) Chromosome measurement and sorting by flow systems. Proc. Natl. Acad. Sci. U. S. A. 72, 1231–1234.
2. Carrano, A.V., Gray, J.W., Langlois, R.G., Burkhart-Schultz, K.J., and Van Dilla, M.A. (1979) Measurement and purification of human chromosomes by flow cytometry and sorting. Proc. Natl. Acad. Sci. U. S. A. 76, 1382–1384.
3. Dixon, S.C., Miller, N.G.A., Carter, N.P., and Tucker, E.M. (1992) Bivariate flow cytometry of farm animal chromosomes: a potential tool for gene mapping. Anim. Genet. 23, 203–210.
4. Ferguson-Smith, M.A. (1997) Genetic analysis by chromosome sorting and painting: phylogenetic and diagnostic applications. Eur. J. Hum. Genet. 5, 253–265.
5. Kulemzina, A.I., Trifonov, V.A., Perelman, P.L., Rubtsova, N.V., Volobuev, V., Ferguson-Smith, M.A., Stanyon, R., Yang, F.T., and Graphodatsky, A.S. (2009) Cross-species chromosome painting in Cetartiodactyla: reconstructing the karyotype evolution in key phylogenetic lineages. Chromosome Res. 17, 419–436.
6. Boschman, G.A., Mandel, E.M.M., Rens, W., Slater, R., and Aten, J.A. (1992) Semi-automated detection of aberrant chromosomes in bivariate flow karyotypes. Cytometry 13, 469–477.
7. Lebo, R.V. (1982) Chromosome sorting and DNA sequence localization: a review. Cytometry 3, 145–154.
8. Van Dilla, M.A., and Deaven, L.L. (1990) Construction of gene libraries for each human chromosome. Cytometry 11, 208–218.
9. Gingrich, J.C., Boehrer, D.M., Garnes, J.A., Johnson, W., Wong, B.S., Bergmann, A., Eveleth G.G., Langlois, R.G., and Carrano, A.V. (1996) Construction and characterization of human chromosome 2-specific cosmid, fos-mid, and PAC clone libraries. Genomics 32, 65–74.
10. Pinkel, D., Landegent, J., Collins, C., Fuscoe, J., Segraves, R., Lucas, J., and Gray J. (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of tri-somy 21 and translocations of chromosome 4. Proc. Natl. Acad. Sci. U. S. A. 85, 9138−9142.
11. Chen, W., Kalscheuer, V., Tzschach, A., Menzel, C., Ullmann, R., Schulz, M.H., Erdogan, F., Li, N., Kijas, Z., Arkesteijn, G., Pajares, I.L., Goetz-Sothmann, M., Heinrich, U., Rost, I., Dufke, A., Grasshoff, U., Glaeser, B., Vingron, M., and Ropers, H.H. (2008) Mapping translocation breakpoints by next-generation sequencing. Genome Res. 18, 1143–1149.
12. Sudbery, I., Stalker, J., Simpson, J.T., Keane, T., Rust, A.G., Hurles, M.E., Walter, K., Lynch, D., Teboul, L., Brown, S.D., Li, H., Ning, Z., Nadeau, J.H., Croniger, C.M., Durbin, R., and Adams D.J. (2009) Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels. Genome Biol. 10, R112.1–R112.19.
13. Doležel, J., Lucretti, S., and Schubert, I. (1994) Plant chromosome analysis and sorting by flow cytometry. Crit. Rev. Plant Sci. 13, 275–309.
14. Doležel, J., Kubaláková, M., Bartoš, J., and Macas, J. (2004) Flow cytogenetics and plant genome mapping. Chromosome Res. 12, 77–91.
15. Doležel, J., Číhalíková, J., and Lucretti, S. (1992) A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L. Planta 188, 93–98.
16. Lysák, M.A., Číhalíková, J., Kubaláková, M., Šimková, H., Künzel, G., and Doležel, J. (1999). Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.). Chromosome Res. 7, 431–444.
17. Vrána, J., Kubaláková, M., Šimková, H., Číhalíková, J., Lysák, M.A., and Doležel, J. (2000) Flow-sorting of mitotic chromosomes in common wheat (Triticum aestivum L.). Genetics 156, 2033–2041.
18. Kubaláková, M., Valárik, M., Bartoš, J., Vrána, J., Číhalíková, J., Molnár-Láng, M., and Doležel, J. (2003) Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry. Genome 46, 893–905.
19. Lucretti, S., and Doležel, J. (1997) Bivariate flow karyotyping in broad bean (Vicia faba). Cytometry 28, 236–242.
20. Kubaláková, M., Vrána, J., Číhalíková, J., Šimková, H., and Doležel, J. (2002) Flow karyotyping and chromosome sorting in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 104, 1362–1372.
21. Suchánková, P., Kubaláková, M., Kovářová, P., Bartoš, J., Číhalíková, J., Molnár-Láng, M., Endo, T.R., and Doležel, J. (2006) Dissection of the nuclear genome of barley by chromosome flow sorting. Theor. Appl. Genet. 113, 651–659.
22. Macas, J., Doležel, J., Lucretti, S., Pich, U., Meister, A., Fuchs, J., and Schubert, I. (1993) Localization of seed storage protein genes on flow-sorted field bean chromosomes. Chromosome Res. 1, 107–115.
23. Kejnovský, E., Vrána, J., Matsunaga, S., Souček, P., Široký, J., Doležel, J., and Vyskot, B. (2001) Localization of male-specifically expressed MROS genes of Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Genetics 158, 1269–1277.
24. Šimková, H., Svensson, J.T., Condamine, P., Hřibová, E., Suchánková, P., Bhat, P.R., Bartoš, J., Šafář, J., Close, T.J., and Doležel, J. (2008) Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley. BMC Genomics 9, 294.
25. Požárková, D., Koblížková, A., Román, B., Torres, A.M., Lucretti, S., Lysák, M., Doležel, J., and Macas, J. (2002) Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba. Biol. Plant. 45, 337–345.
26. Kofler, R., Bartoš, J., Gong, L., Stift, G., Suchánková, P., Šimková, H., Berenyi, M., Burg, K., Doležel, J., and Lelley, T. (2008) Development of microsatellite markers specific for the short arm of rye (Secale cereale L.) chromosome 1. Theor. Appl. Genet. 117, 915–926.
27. Šafář, J., Bartoš, J., Janda, J., Bellec, A., Kubaláková, M., Valárik, M., Pateyron, S., Weiserová, J., Tušková, R., Číhalíková, J., Vrána, J., Šimková, H., Faivre-Rampant, P., Sourdille, P., Caboche, M., Bernard, M., Doležel, J., and Chalhoub, B. (2004) Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat. Plant J. 39, 960–968.
28. Šimková, H., Šafář, J., Suchánková, P., Kovářová, P., Bartoš, J., Kubaláková, M., Janda, J., Číhalíková, J., Mago, R., Lelley, T., and Doležel, J. (2008) A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS). BMC Genomics 9, 237.
29. Mayer, K.F.X., Taudien, S., Martis, M., Šimková, H., Suchánková, P., Gundlach, H., Wicker, T., Petzold, A., Felder, M., Steuernagel, B., Scholz, U., Graner, A., Platzer, M., Doležel, J., and Stein N. (2009) Gene content and virtual gene order of barley chromosome 1H. Plant Physiol. 151, 496–505.