Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry

Methods in Molecular Biology

Volumn 708, Issue , 2011, Pages 131-146

  van Dam, Jan C ^a   Ras, Cor ^a   ten Pierick, Angela ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

anion exchange chromatography; GC MS; Glycolytic intermediates; IC MS MS; ion suppressor; isotope dilution mass spectrometry; TMS MOX derivatives

Indexed keywords

CARBOHYDRATE;

ARTICLE; CHEMISTRY; GLYCOLYSIS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS FRAGMENTOGRAPHY; METHODOLOGY; STANDARD;

CARBOHYDRATES; CHROMATOGRAPHY, ION EXCHANGE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLYCOLYSIS; REFERENCE STANDARDS;

EID: 79953748888     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-985-7_7     Document Type: Chapter

References (4)

1. van Dam, J. C., Eman, M. R., Frank, J., Lange, H. C., van Dedem, G. W. K., Heijnen, S. J. (2002) Analysis of glycolytic intermediates in Saccharomyces cerevisiae using anion exchange chromatography and electrospray ionization with tandem mass spectrometric detection. Anal Chim Acta 460, 209–218.
2. Cipollina, C., ten Pierick, A., Canelas, A. B., Seifar, R. M., van Maris, A. J. A., van Dam, J. C., nd Heijnen, J. J. (2009) A comprehensive method for the quantification of the non-oxidative pentose phosphate pathway intermediates in Saccharomyces cerevisiae by GC-IDMS. J Chromatogr B 877, 3231–3236.
3. Canelas, A. B., Ras, C., ten Pierick, A., Seifar, R., van Dam, J. C., van Gulik, W. M., Heijnen, J. J. (2009) Quantitative evaluation of intracellular metabolite extraction techniques for microbial metabolomics. Anal Chem 81, 7379–7389
4. Mashego, M. R., Wu, L., van Dam, J. C., Ras, C., Vinke, J. L., Van Winden, W. A., Van Gulik, W. M., Heijnen, J. J. (2004) MIRACLE: mass isotopomer ratio analysis of U-C-13-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites. Biotechnol Bioeng 85, 620–628.