Proteomic approaches to identifying carbonylated proteins in brain tissue

Journal of Proteome Research

Volumn 10, Issue 4, 2011, Pages 1719-1727

  Linares, María ^a,b   Marín García, Patricia ^a,b,c   Méndez, Darío ^a,b,d   Puyet, Antonio ^a,b   Diez, Amalia ^a,b   Bautista, José M ^a,b  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b HOSPITAL UNIVERSITARIO 12 DE OCTUBRE   (Spain)

^c UNIVERSIDAD EUROPEA DE MADRID   (Spain)

^d UNIVERSIDAD DE CARTAGENA   (Colombia)

Author keywords

2,4 dinitrophenylhydrazine; 2D oxyblot; carbonylation; cerebellum; protein oxidation; Redox proteomics

Indexed keywords

2,4 DINITROPHENYLHYDRAZINE; 2,4 DINITROPHENYLHYDRAZONE; 3 OXOACID COENZYME A TRANSFERASE; ADENOSINE TRIPHOSPHATASE; ALPHA ENOLASE; BRAIN PROTEIN; COLLAPSIN RESPONSE MEDIATOR PROTEIN; COLLAPSIN RESPONSE MEDIATOR PROTEIN 2; CREATINE KINASE; DIHYDROPYRIMIDINASE RELATED PROTEIN 4; ELECTRON TRANSFERRING FLAVOPROTEIN; GLUTAMATE AMMONIA LIGASE; GLUTAMATE DEHYDROGENASE; HYDRAZONE DERIVATIVE; LONG CHAIN ACYL COENZYME A DEHYDROGENASE; MALATE DEHYDROGENASE; NEURON SPECIFIC ENOLASE; PHOSPHOPROTEIN; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; PYRUVATE KINASE; UNCLASSIFIED DRUG; VIMENTIN;

ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; MASS SPECTROMETRY; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CARBONYLATION;

EID: 79953712953     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr101014e     Document Type: Article

References (29)

1. Butterfield, D. A.; Reed, T.; Newman, S. F.; Sultana, R. Roles of amyloid beta-peptide-associated oxidative stress and brain protein modifications in the pathogenesis of Alzheimers disease and mild cognitive impairment Free Radical Biol. Med. 2007, 43 (5) 658-77 (Pubitemid 47102132)
2. Cecarini, V.; Gee, J.; Fioretti, E.; Amici, M.; Angeletti, M.; Eleuteri, A. M.; Keller, J. N. Protein oxidation and cellular homeostasis: Emphasis on metabolism Biochim. Biophys. Acta 2007, 1773 (2) 93-104 (Pubitemid 46123609)
3. Dalle-Donne, I.; Rossi, R.; Giustarini, D.; Milzani, A.; Colombo, R. Protein carbonyl groups as biomarkers of oxidative stress Clin. Chim. Acta 2003, 329 (1-2) 23-38 (Pubitemid 36187411)
4. Shacter, E. Quantification and significance of protein oxidation in biological samples Drug Metab. Rev. 2000, 32 (3-4) 307-26 (Pubitemid 32001909)
5. Madian, A. G.; Regnier, F. E. Proteomic identification of carbonylated proteins and their oxidation sites J. Proteome Res. 2010, 9 (8) 3766-80
6. Dalle-Donne, I.; Aldini, G.; Carini, M.; Colombo, R.; Rossi, R.; Milzani, A. Protein carbonylation, cellular dysfunction, and disease progression J. Cell. Mol. Med. 2006, 10 (2) 389-406 (Pubitemid 44870187)
7. Levine, R. L.; Williams, J. A.; Stadtman, E. R.; Shacter, E. Carbonyl assays for determination of oxidatively modified proteins Methods Enzymol. 1994, 233, 346-57
8. Dalle-Donne, I.; Rossi, R.; Colombo, R.; Giustarini, D.; Milzani, A. Biomarkers of oxidative damage in human disease Clin. Chem. 2006, 52 (4) 601-23
9. Shacter, E. Protein oxidative damage Methods Enzymol. 2000, 319, 428-36 (Pubitemid 30448062)
10. Conrad, C. C.; Choi, J.; Malakowsky, C. A.; Talent, J. M.; Dai, R.; Marshall, P.; Gracy, R. W. Identification of protein carbonyls after two-dimensional electrophoresis Proteomics 2001, 1 (7) 829-34 (Pubitemid 33587408)
11. Reinheckel, T.; Korn, S.; Mohring, S.; Augustin, W.; Halangk, W.; Schild, L. Adaptation of protein carbonyl detection to the requirements of proteome analysis demonstrated for hypoxia/reoxygenation in isolated rat liver mitochondria Arch. Biochem. Biophys. 2000, 376 (1) 59-65 (Pubitemid 30205114)
12. Butterfield, D. A.; Abdul, H. M.; Newman, S.; Reed, T. Redox proteomics in some age-related neurodegenerative disorders or models thereof NeuroRx 2006, 3 (3) 344-57 (Pubitemid 43946717)
13. Bruno, M.; Moore, T.; Nesnow, S.; Ge, Y. Protein carbonyl formation in response to propiconazole-induced oxidative stress J Proteome Res. 2009, 8 (4) 2070-8
14. Marsano, F.; Boatti, L.; Ranzato, E.; Cavaletto, M.; Magnelli, V.; Dondero, F.; Viarengo, A. Effects of mercury on Dictyostelium discoideum: proteomics reveals the molecular mechanisms of physiological adaptation and toxicity J. Proteome Res. 2010, 9 (6) 2839-54
15. Radfar, A.; Diez, A.; Bautista, J. M. Chloroquine mediates specific proteome oxidative damage across the erythrocytic cycle of resistant Plasmodium falciparum Free Radical Biol. Med. 2008, 44 (12) 2034-42
16. Sultana, R.; Newman, S. F.; Huang, Q.; Butterfield, D. A. Detection of carbonylated proteins in two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis separations Methods Mol. Biol. 2009, 476, 149-59
17. Tanou, G.; Job, C.; Belghazi, M.; Molassiotis, A.; Diamantidis, G.; Job, D. Proteomic signatures uncover hydrogen peroxide and nitric oxide cross-talk signaling network in citrus plants J. Proteome Res. 2010, 9, 5994-6006
18. Castegna, A.; Aksenov, M.; Aksenova, M.; Thongboonkerd, V.; Klein, J. B.; Pierce, W. M.; Booze, R.; Markesbery, W. R.; Butterfield, D. A. Proteomic identification of oxidatively modified proteins in Alzheimers disease brain. Part I: creatine kinase BB, glutamine synthase, and ubiquitin carboxy-terminal hydrolase L-1 Free Radical Biol. Med. 2002, 33 (4) 562-71 (Pubitemid 34874977)
19. Tezel, G.; Yang, X.; Cai, J. Proteomic identification of oxidatively modified retinal proteins in a chronic pressure-induced rat model of glaucoma Invest. Ophthalmol. Vision Sci. 2005, 46 (9) 3177-87 (Pubitemid 44264303)
20. Korolainen, M. A.; Goldsteins, G.; Nyman, T. A.; Alafuzoff, I.; Koistinaho, J.; Pirttila, T. Oxidative modification of proteins in the frontal cortex of Alzheimers disease brain Neurobiol. Aging 2006, 27 (1) 42-53 (Pubitemid 41627949)
21. Sultana, R.; Boyd-Kimball, D.; Poon, H. F.; Cai, J.; Pierce, W. M.; Klein, J. B.; Merchant, M.; Markesbery, W. R.; Butterfield, D. A. Redox proteomics identification of oxidized proteins in Alzheimers disease hippocampus and cerebellum: an approach to understand pathological and biochemical alterations in AD Neurobiol. Aging 2006, 27 (11) 1564-76 (Pubitemid 44344840)
22. Conrad, C. C.; Talent, J. M.; Malakowsky, C. A.; Gracy, R. W. Post-electrophoretic identification of oxidized proteins Biol. Proced. Online 2000, 2, 39-45
23. Singh, N. R.; Rondeau, P.; Hoareau, L.; Bourdon, E. Identification of preferential protein targets for carbonylation in human mature adipocytes treated with native or glycated albumin Free Radical Res. 2007, 41 (10) 1078-88 (Pubitemid 47470845)
24. Fedorova, M.; Kuleva, N.; Hoffmann, R. Identification of cysteine, methionine and tryptophan residues of actin oxidized In Vivo during oxidative stress J. Proteome Res. 2010, 9 (3) 1598-609
25. Neuhoff, V.; Stamm, R.; Eibl, H. Clear background and highly sensitive protein staining with Coomassie blue dyes in polyacrylamide gels-a systematic analysis Electrophoresis 1985, 6, 427-448 (Pubitemid 15023889)
26. Shevchenko, A.; Wilm, M.; Vorm, O.; Mann, M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels Anal. Chem. 1996, 68 (5) 850-8
27. Churchward, M. A.; Butt, R. H.; Lang, J. C.; Hsu, K. K.; Coorssen, J. R. Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis Proteome Sci. 2005, 3 (1) 5
28. Oikawa, S.; Yamada, T.; Minohata, T.; Kobayashi, H.; Furukawa, A.; Tada-Oikawa, S.; Hiraku, Y.; Murata, M.; Kikuchi, M.; Yamashima, T. Proteomic identification of carbonylated proteins in the monkey hippocampus after ischemia-reperfusion Free Radical Biol. Med. 2009, 46 (11) 1472-7
29. Talent, J. M.; Kong, Y.; Gracy, R. W. A double stain for total and oxidized proteins from two-dimensional fingerprints Anal. Biochem. 1998, 263 (1) 31-8 (Pubitemid 28445421)