Direct PCR-based method for detecting Bursaphelenchus xylophilus, the pine wood nematode in wood tissue of Pinus massoniana

Forest Pathology

Volumn 41, Issue 2, 2011, Pages 165-168

  Hu, Y Q ^a,d   Kong, X C ^a   Wang, X R ^a   Zhong, T K ^b   Zhu, X W ^a   Mota, M M ^c   Ren, L L ^a   Liu, S ^a   Ma, C ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b Forest Pest Management and Quarantine Station of Guangzhou   (China)

^c UNIVERSITY OF ÉVORA   (Portugal)

^d YICHUN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CONIFEROUS FOREST; NEMATODE; PARASITIC DISEASE; POLYMERASE CHAIN REACTION; VULNERABILITY; WILT; WOOD;

CHINA;

BURSAPHELENCHUS; BURSAPHELENCHUS XYLOPHILUS; PINUS MASSONIANA;

EID: 79953325703     PISSN: 14374781     EISSN: 14390329     Source Type: Journal    
DOI: 10.1111/j.1439-0329.2010.00692.x     Document Type: Article

References (23)

1. Braasch, H.; Burgermeister, W.; Gu, J., 2009: Revised intra-generic grouping of Bursaphlenchus Fuchs, 1937 (Nematoda: Aphelenchoididae). J. Nematode Morphol. System. 12, 65-88.
2. Cao, A.X.; Liu, X.Z.; Zhu, S.F.; Lu, B.S., 2005: Detection of the pinewood nematode, Bursaphelenchus xylophilus, using a real-time PCR polymerase chain reaction assay. Phytopathology 95, 566-571.
3. Castagnone, C; Abad, P.; Castagnone-Sereno, P., 2005: Satellite DNA-based species-specific identification of single individuals of the pinewood nematode Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Eur. J. Plant Pathol. 112, 191-193.
4. Chen, F. M.; Tang, J.; Ye, J. Z., 2005: Pine wilt disease diagnostic method assessment. J. Anhui Agric. Univ. 32, 22-25.
5. François, C.; Castagnone, C.; Boonham, N.; Tomlinsonr, R.; Lawson, C.; Hockland, S.; Quill, J.; Vieira, P.; Mota, M.; Castagnone-Sereno, P., 2007: Satellite DNA as a target for TaqMan real-time PCR detection of the pine wood nematode, Bursaphelenchus xylophilus. Mol. Plant Pathol. 8, 803-809.
6. Hoyer, U.; Burgermeister, W.; Braasch, H., 1998: Identification of Bursaphelenchus species (Nematode, Aphelenchoididae) on the basic of amplified ribosomal DNA(ITS-RFLP). Nachr Dtsch Pflanzenschutzd 50, 273-277.
7. Iwahori, H.; Kanzaki, N.; Futa, I. K., 2000: A simple polymerase chain reaction-restriction fragment length polymorphism-aided diagnosis method for pine wilt disease. For. Pathol. 3, 157-164.
8. Kang, J. S.; Choi, K. S.; Shin, S. C.; Moon, I. S.; Lee, S. G.; Lee, S. H., 2004: Development of an efficient PCR-based diagnosis protocol for identification of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Nematology 6, 279-285.
9. Kikuchi, T.; Aikawa, T.; Oeda, Y.; Karim, N.; Kanzaki, N., 2009: A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology 99, 1365-1369.
10. Leal, I.; Green, M.; Allen, E.; Humble, L.; Rott, M., 2005: An effective PCR-based diagnostic method for the detection of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) in wood samples from lodgepole pine. Nematology 7, 833-842.
11. Leal, I.; Margaret, G.; Eric, A.; Leland, H.; Michael, R., 2007: Application of a real-time PCR method for the detection of pine wood nematode, Bursaphelenchus xylophilus, in wood samples from lodgepole pine. Nematology 9, 351-362.
12. Liao, J. L.; Zhang, L. H.; Feng, Z. X., 2001: A reliable Identification of Bursaphelenchus xylophilus by rDNA amplification. Nematol. Mediterr. 29, 131-135.
13. Mamiya, Y., 1975: Efficiency of Baermann funnel method for the extraction of pinewood nematodes. Forest Pest. 24, 115-119.
14. Matsunaga, K.; Togashi, K., 2004: A simple method for discriminating Bursaphelenchus xylophilus and B. mucronatus by species-specific polymerase chain reaction primer pairs. Nematology 6, 273-277.
15. Schena, L.; Hughes, K. J. D.; Cooke, D. E. L., 2006: Detection and quantification of P. ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real-time PCR. Mol. Plant Pathol. 7, 365-379.
16. Schröder, T.; McNamara, D. G.; Gaar, V., 2009: Guidance on sampling to detect pine wood nematode Bursaphelenchus xylophilus in trees, wood and insects. Bull. OEPP 39, 179-188.
17. Takeuchi, Y.; Futai, K., 2007: A symptomatic carrier trees in pine stands naturally infected with Bursaphelenchus xylophilus. Nematology 9, 243-250.
18. Takeuchi, Y.; Futai, K., 2009: Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhner), in wood of Pinus thunbergii with real-time PCR. Nematol. Res. 39, 9-16.
19. Takeuchi, Y.; Kanzaki, N.; Futai, K., 2005: A nested PCR-based method for detecting the pine wood nematode, Bursaphelenchus xylophilus, from pine wood. Nematology 7, 775-782.
20. Wang, X. R.; Zhu, X. W.; Hu, Y. Q.; Huang, H. H.; Kong, X. C.; Jia, W. H., 2009: A PCR-based method for detecting the pine wood nematodes -Bursaphelenchus xylophilus from Monochamus alternatus. Sci. Silvae Sin. 45, 70-75.
21. Wu, X. Q.; Xiong, D.; An, Y., 2005: RAPD Analysis of genetic relationships in inter and inner-species of Bursaphelenchus xylophilus and B. mucronatus. J. Nanjing For. Univ. (Natural Science Edition). 29, 1-4.
22. Zhao, L.L.; Wei, W; Kang, L.; Sun, J. H., 2007: Chemotaxis of the Pinewood Nematode, Bursaphelenchus xylophilus, to Volatiles Associated with Host Pine, Pinus massoniana, and its Vector Monochamus alternatus. J. Chem. Ecol. 33, 1207-1216.
23. Zhuo, K.; Luo, M.; Cui, R.Q; Liao, J. J. 2010: A multiplex one-step PCR method for the simultaneous identification of Bursaphelenchus xylophilus, B. mucronatus and B. doui- three species within the "xylophilus" group. For. Pathol. Doi.