Cloning, soluble expression and purification of high yield recombinant hGMCSF in Escherichia coli

International Journal of Molecular Sciences

Volumn 12, Issue 3, 2011, Pages 2064-2076

  Das, Krishna M P ^a   Banerjee, Sampali ^a   Shekhar, Nivedita ^a   Damodaran, Karpagavalli ^a   Nair, Rahul ^a   Somani, Sandeep ^a   Raiker, Veena P ^a   Jain, Shweta ^a   Padmanabhan, Sriram ^a  

^a LUPIN LIMITED   (India)

Author keywords

Enterokinase; Human granulocyte macrophage colony stimulating factor; IMAC; On column cleavage; TRX fusion

Indexed keywords

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; THIOREDOXIN; HYBRID PROTEIN;

ARTICLE; BIOREACTOR; CONTROLLED STUDY; DRUG DETERMINATION; DRUG PURIFICATION; DRUG SCREENING; ESCHERICHIA COLI; HUMAN; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; BATCH CELL CULTURE; BIOSYNTHESIS; CHEMISTRY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ESCHERICHIA COLI;

BATCH CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; CLONING, MOLECULAR; ESCHERICHIA COLI; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; RECOMBINANT FUSION PROTEINS; THIOREDOXINS;

EID: 79953323310     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12032064     Document Type: Article

References (27)

1. Schwanke, R.C.; Renard, G.; Chies, J.M.; Campos, M.M.; Batista, E.L., Jr.; Santos, D.S.; Basso, L.A. Molecular cloning, expression in Escherichia coli and production of bioactive homogeneous recombinant human granulocyte and macrophage colony stimulating factor. Int. J. Biol. Macromol. 2009, 45, 97-102.
2. Armitage, J.O. Emerging applications of recombinant human granulocyte-macrophage colony-stimulating factor. EMBO J. 1985, 4, 2575-2581.
3. DeLamarter, J.F.; Mermod, J.J.; Liang, C.M.; Eliason, J.F.; Thatcher, D.R. Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum. EMBO J. 1985, 4, 2575-2581.
4. Bhopale, G.M.; Nanda, R.K. Recombinant DNA expression products for human therapeutic use. Curr. Sci. 2005, 89, 614-622.
5. Berrow, N.S.; Bussow, K.; Coutard, B.; Diprosw, J.; Ekberg, M. Recombinant protein expression and solubility screening in E. coli: A comparative study. Acta Crystallogr. 2006, 67, 1218-1226.
6. Sorenson, H.P.; Mortensen, K.K. Advanced genetic strategies for recombinant protein expression in E. coli. Biotechnology 2005, 115, 13-28.
7. Sahdev, S.; Khattar, S.K.; Saini, K.S. Production of active eukaryotic proteins through bacterial expression system: A review of the existing biotechnology strategies. Mol. Cell Biochem. 2008, 307, 249-264.
8. Yin, J.; Li, G.; Ren, X.; Herrler, G.A. Comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J. Biotechnol. 2007, 127, 335-347.
9. Valeria, D.M.; Gunter, S.; Stephanie, B.; Ario, D.M. The solubility and stability of recombinant proteins are increased by their fusion to NusA. Biochem. Biophys. Res. Commun. 2004, 322, 766-771.
10. Cabrita, L.D.; Dai, W.; Bottomley, S.P. A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol. 2006, 6, 12-19.
11. Srinivasa, B.K.; Muthukumaran, T.; Antony, A.; Samuel, S.D.P.S.; Balamurali, M.; Murugan, V.; Meenakshisundaram, S. Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli. Biotechnol. Lett. 2009, 31, 659-664.
12. Lee, A.Y.; Chung, H.K.; Kyong Bae, E.; Hwang, J.S.; Sung, B.W.; Cho, C.W.; Kim, J.K.; Lee, K.; Han, J.Y.; Lee, C.T.; Youn, H.J. A recombinant human G-CSF/GM-CSF fusion protein from E. coli showing colony stimulating activity on human bone marrow cells. Biotechnol. Lett. 2003, 25, 205-211.
13. Smith, H.E. Transcriptional response of E. coli to recombinant protein insolubility. J. Struct. Funct. Genomics 2007, 8, 27-35.
14. Banerjee, S.; Apte-Deshpande, A.; Mandi, N.; Padmanabhan, S. A novel cytokine derived fusion tag for over-expression of heterologous proteins in E. coli. Int. J Biol. Life Sci. 2009, 1, 139-143.
15. Salunkhe, S.; Prasad, B.; Sabnis-Prasad, K.; Apte-Deshpande, A.; Padmanabhan, S. Expression and purification of SAK-fused human interferon alpha in Escherichia coli. J. Microbial. Biochem. Technol. 2009, 1, 5-10.
16. Harrison, R.G. Expression of soluble heterologous proteins via fusion with NusA protein. Innovations 2000, 11, 4-7.
17. Ackland, C.E.; Berndt, W.G.; Frezza, J.E.; Landgraf, B.E.; Pritchard, K.W.; Ciardelli, T.L. Monitoring of protein conformation by high-performance size-exclusion liquid chromatography and scanning diode array second-derivative UV absorption spectroscopy. J. Chromatogr. A 1991, 540, 187-198.
18. Kaushansky, K.; O'Hara, P.J.; Berkner, K.; Segal, G.M.; Hagen, S.; Adamson, J.W. Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor. Proc. Natl. Acad. Sci. USA 1986, 83, 3101-3105.
19. Moonen, P. Increased biological activity of deglycosylated recombinant human granulocyte/ macrophage colony-stimulating factor produced by yeast or animal cells. Proc. Natl. Acad. Sci. USA 1987, 84, 4428-4431.
20. Wong, G.G.; Witek, J.S.; Temple, P.A.; Wilkens, K.M.; Leary, A.C.; Luxenberg, D.P.; Jones, S.S.; Brown, E.L.; Kay, R.M.; Orr, E.C. Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 1985, 228, 810-815.
21. Miyajima, A.; Otsn, K.; Schreurs, J.; Bond, M.W.; Abrams, J.S.; Arai, K. Expression of murine and human granulocyte-macrophage colony-stimulating factors in S. cerevisiae: Mutagenesis of the potential glycosylation sites. EMBO J. 1986, 5, 1193-1197.
22. Burgess, A.W.; Begley, C.G.; Johnson, G.R.; Lopez, A.F.; Williamson, D.J.; Mermod, J.J.; Simpson, R.J.; Schmitz, A.; de Lamarter, J.F. Purification and properties of bacterially synthesized human granulocyte-macrophage colony stimulating factor. Blood 1987, 69, 43-51.
23. Belew, M.; Zhou, Y.; Wang, S.; Nystrom, L.E.; Janson, J.C. Purification of recombinant human granulocyte-macrophage colony-stimulating factor from the inclusion bodies produced by transformed Escherichia coli cells. J. Chromatogr. 1994, 679, 67-83.
24. Srinivasa, B.K.; Antony, A.; Muthukumaran, T.; Meenakshisundaram, S. Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris. Protein Expr. Purif. 2008, 57, 201-205.
25. Cowgill, C.; Ozturk, A.G.; John, R. Protein Refolding and Scale Up; Shukla, A.A., Etzel, M.R., Gadam, S., Eds.; Taylor & Francis: New York, NY, USA, 2007; pp. 124-158.
26. Sletta, H.; Tondervik, A.; Hakvag, S.; Aune, T.E.V.; Nedal, A.; Aune, R.; Evensen, G.; Valla, S.; Ellingsen, T.E.; Brautaset, T. The presence of N-terminal Secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli. Appl. Environ. Microbiol. 2007, 73, 906-912.
27. Mandi, N.; Soorapaneni, S.; Rewanwar, S.; Kotwal, P.; Prasad, B.J.; Mandal, G.; Padmanabhan, S. High-yielding recombinant staphylokinase in bacterial expression system-cloning, expression, purification and activity studies. Protein Expr. Purif. 2009, 64, 69-75.