New naphthoquinones and a new δ-lactone produced by endophytic fungi from Costa Rica

Tetrahedron Letters

Volumn 52, Issue 17, 2011, Pages 2206-2208

  Cao, Shugeng ^a   Clardy, Jon ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Lactones; Anti malaria; Endophytic fungi; Naphthoquinones

Indexed keywords

ANTIMALARIAL AGENT; DELITZCHIANONE; LACTONE DERIVATIVE; NAPHTHOQUINONE; UNCLASSIFIED DRUG;

ANTIMALARIAL ACTIVITY; ARTICLE; COSTA RICA; ENDOPHYTE; FUNGUS; IC 50; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE;

FUNGI; PHOMATOSPORA;

EID: 79953224409     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2010.11.159     Document Type: Article

References (11)

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4. Sequencing and species identification. For identification by internal transcribed spacer (ITS) sequencing, CR237A and CR1092F were cultured in potato dextrose broth for 5 days. The mycelium was then retrieved by filtration and ground to a fine powder in liquid N2. Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega), and large subunit rDNA was amplified by PCR using primers LR5 (5′-TCCTGAGGGAAACTTCG-3′) and LROR (5′-ACCCGCTGAACTTAAGC-3′). PCR products were transformed into E. coli TOP10 cells using a TOPO TA Cloning Kit (Invitrogen), according to manufacturer's protocols. Transformed plasmids were isolated and sequenced at Genewiz (http://www.genewiz.com/). The following consensus sequence were used in a BLAST search against deposited sequences: 237A: CCCCTATGCCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCT CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC CCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATGGTG CGCCAGAGCTCGCGCCCTGGGTCCCACCTCCGTTCACTTTCATTCCGCGCCCGGGCTT GACACCCAAACACTCGCATAGATGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGCC GCTTACGACCATTACGCCAGCATCCTAGCCGAAGCGCGGACCTCAGTCGGGGCTGG CTGCATGACGCCCTGGGCTATAACACTCCCCGAAGAGAGCTACATTCCCAAGGCCTT TCTCCAGCCGCCCCAACTGATGCTGGCCTGCCTGCCGCCGAGTGCACAGGGGACGG ACCCCCGATGAACAGCGGCAGCCAAGTCTGGTTGCAAGCGCTTCCCTTTCAACAATT TCACGTGCTGTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTACTTGT GCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATATACCTCCCATTTAGA GCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGGTTCACATGGCGCAGGCACCTG CCGCGTACGGGGTTCTCACCCTCTCTGACGTCCCGTTCCAAGGAACTTAGACAGGCGN CGTTGCCGAACCACCNTCTGCAAAGTACAACTCGGANCCCGCAAGGAGCCAGATTTC AAATTTGAGCTGTTGCCGCTTCACTCGCCGTTACT GAGGCAAT CR1092F: AGAGGTTGATAGTCTTTGCCCCCATGCTCATGTTTGACGATCGATTTGCA CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTACACAAGC ATAGTTCACCATCTTTCGGGTCCAAGCGGCAAGGCTCTTACTCAAATCCATCCGAAG ACTTCAGGATCGGTCGATGGTGCGCCGAmAGAGGTTGATAGTCTTTCGCCCCCATG CTCATGTTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACCAGAGTTT CCTCTGGCTAGAGGTTGATAGTCTTTCGCCCCCATGCTCATGTTTGACGATCGATTTG CACGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTACACAA GCATAGTTCACCATCTTTCGGGTCCAAGCGGCAAGGCTCTTACTCAAATCCATCCGA AGACTTCAGGATCGGTCGATGGTGCGCCGAmAGAGGTTGATAGTCTTTCGCCCCCA TGCTCATGTTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACCAGAGT TTCCTCTGGCTAGAGGTTGATAGTCTTTCGCCCCCATGCTCATGTTTGACGATCGATT TGCACGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTACAC AAGCATAGTTCACCATCTTTCGGGTCCAAGCGGCAAGGCTCTTACTCAAATCCATCC GAAGACTTCAGGATCGGTCGATGGTGCGCCGAmAGAGGTTGATAGTCTTTCGCCCC CATGCTCATGTTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACCAGA GTTTCCTCTGGCT AGAGGTT TTTCAANCGGGGTAA.
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