Development of an aerosol surface inoculation method for Bacillus spores

Applied and Environmental Microbiology

Volumn 77, Issue 5, 2011, Pages 1638-1645

  Lee, Sang Don ^a   Ryan, Shawn P ^a   Snyder, Emily Gibb ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AEROSOLIZATION; BACILLUS ANTHRACIS; BACILLUS SPORE; BACILLUS SUBTILIS SPORES; BIOLOGICAL AGENTS; COEFFICIENT OF VARIATION; DEPOSITION METHODS; DEPOSITION PATTERNS; GALVANIZED STEELS; INOCULATION METHOD; MATERIAL SURFACE; METERED-DOSE INHALERS; SURFACE MATERIALS;

ATMOSPHERIC AEROSOLS; BACILLI; BACTERIOLOGY; BIOLOGICAL MATERIALS; BUILDING MATERIALS; DECONTAMINATION; DEPOSITS; GALVANIZED METAL; GALVANIZING; SCANNING ELECTRON MICROSCOPY; SURFACES; WALLS (STRUCTURAL PARTITIONS);

ALUMINUM COATINGS;

AEROSOL; BACTERIUM; EXPERIMENTAL STUDY; SCANNING ELECTRON MICROSCOPY; SPORE;

AEROSOL; ARTICLE; BACILLUS SUBTILIS; BACTERIAL SPORE; EQUIPMENT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; REPRODUCIBILITY;

AEROSOLS; BACILLUS SUBTILIS; BACTERIOLOGICAL TECHNIQUES; ENVIRONMENTAL MICROBIOLOGY; EQUIPMENT CONTAMINATION; REPRODUCIBILITY OF RESULTS; SPORES, BACTERIAL;

BACILLUS ANTHRACIS; BACILLUS SUBTILIS;

EID: 79953222241     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02237-10     Document Type: Article

References (21)

1. ASTM International. 2002. ASTM standard E2197, Standard quantitative disk carrier test method for determining the bactericidal, virucidal, fungicidal, mycobactericidal, and sporicidal activities of liquid chemical germicides. ASTM International, West Conshohocken, PA. doi:10.1520/E2197-02, http://www.astm.org/Standards/E2197.htm.
2. Baron, P. A., et al. 2008. Development of an aerosol system for uniformly depositing Bacillus anthracis spore particles on surfaces. Aerosol Sci. Technol. 42:159-172.
3. Beecher, D. J. 2006. Forensic application of microbiological culture analysis to identify mail intentionally contaminated with Bacillus anthracis spores. Appl. Environ. Microbiol. 72:5304-5310.
4. Brown, G. S., et al. 2007. Evaluation of a wipe surface sample method for collection of Bacillus spores from nonporous surfaces. Appl. Environ. Microbiol. 73:706-710.
5. Brown, G. S., et al. 2007. Evaluation of vacuum filter sock surface sample collection method for Bacillus spores from porous and non-porous surfaces. J. Environ. Monit. 9:666-671.
6. Brown, G. S., et al. 2007. Evaluation of rayon swab surface sample collection method for Bacillus spores from nonporous surfaces. J. Appl. Microbiol. 103:1074-1080.
7. Carrera, M., J. Kesavan, R. Zandomeni, and J. L. Sagripanti. 2005. Method to determine the number of bacterial spores within aerosol particles. Aerosol Sci. Technol. 39:960-965.
8. Carrera, M., R. O. Zandomeni, and J. L. Sagripanti. 2008. Wet and dry density of Bacillus anthracis and other Bacillus species. J. Appl. Microbiol. 105:68-77.
9. Dull, P. M., et al. 2002. Bacillus anthracis aerosolization associated with a contaminated mail sorting machine. Emerg. Infect. Dis. 8:1044-1047.
10. Edmonds, J. M., et al. 2009. Surface sampling of spores in dry-deposition aerosols. Appl. Environ. Microbiol. 75:39-44.
11. Estill, C. F., et al. 2009. Recovery efficiency and limit of detection of aerosolized Bacillus anthracis Sterne from environmental surface samples. Appl. Environ. Microbiol. 75:4297-4306.
12. Gibb-Snyder, E., B. Gullett, S. Ryan, L. Oudejans, and A. Touati. 2006. Development of size-selective sampling of Bacillus anthracis surrogate spores from simulated building air intake mixtures for analysis via laserinduced breakdown spectroscopy. Appl. Spectrosc. 60:860-870.
13. Goldstein, J., et al. 1992. Scanning electron microscopy and X-ray microanalysis, 2nd ed. Kluwer Academic/Plenum, New York, NY.
14. Heimbuch, B. K., K. Kinney, B. Nichols, and J. D. Wander. 2009. The dry aerosol deposition device (DADD): an instrument for depositing microbial aerosols onto surfaces. J. Microbiol. Methods 78:255-259.
15. Rastogi, V. K., L. Wallace, L. S. Smith, S. P. Ryan, and B. Martin. 2009. Quantitative method to determine sporicidal decontamination of building surfaces by gaseous fumigants, and issues related to laboratory-scale studies. Appl. Environ. Microbiol. 75:3688-3694.
16. Rogers, J. V., et al. 2005. Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator. J. Appl. Microbiol. 99:739-748.
17. Rose, L., B. Jensen, A. Peterson, S. N. Banerjee, and M. J. Srduino. 2004. Swab materials and Bacillus anthracis spore recovery from nonporous surfaces. Emerg. Infect. Dis. 10:1023-1029.
18. Sanderson, W. T., et al. 2004. Bacillus anthracis contamination and inhalational anthrax in a mail processing and distribution center. J. Appl. Microbiol. 96:1048-1056.
19. Teshale, E. H., et al. 2002. Environmental sampling for spores of Bacillus anthracis. Emerg. Infect. Dis. 8:1083-1087.
20. Tomasino, S. F., et al. 2010. Use of alternative carrier materials in AOAC official method 2008.05, efficacy of liquid sporicides against spores of Bacillus subtilis on a hard, nonporous surface, quantitative three-step method. J. AOAC Int. 93:259-276.
21. Weis, C. P., et al. 2002. Secondary aerosolization of viable Bacillus anthracis spores in a contaminated US Senate office. JAMA 288:2853-2858.