Integrative expression system for delivery of antibody fragments by lactobacilli

Applied and Environmental Microbiology

Volumn 77, Issue 6, 2011, Pages 2174-2179

  Martín, M Cruz ^a   Pant, Neha ^b   Ladero, Victor ^a   Günaydin, Gökçe ^b   Andersen, Kasper Krogh ^b   Álvarez, Beatriz ^b   Martínez, Noelia ^a   Alvarez, Miguel A ^a   Hammarström, Lennart ^b   Marcotte, Harold ^b  

^a DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY OF DAIRY PRODUCTS   (Spain)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY FRAGMENT; EXPRESSION CASSETTE; EXPRESSION SYSTEM; HEAVY CHAIN; MOUSE MODELS; ROTAVIRUSES; SURFACE DISPLAYS; VARIABLE DOMAIN;

ANTIBODIES;

APF PROTEIN, LACTOBACILLUS CRISPATUS; BACTERIAL PROTEIN; IMMUNOGLOBULIN FRAGMENT;

ANTIBODY; BACTERIUM; CHROMOSOME; FRAGMENTATION; GENE EXPRESSION; NUMERICAL MODEL;

ANIMAL; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; IMMUNOLOGY; LACTOBACILLUS; LACTOBACILLUS FERMENTUM; MOUSE; PHYSIOLOGY; ROTAVIRUS INFECTION;

ANIMALS; BACTERIAL PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN FRAGMENTS; LACTOBACILLUS; LACTOBACILLUS FERMENTUM; MICE; ROTAVIRUS INFECTIONS;

LACTOBACILLUS PARACASEI; LAMA (MAMMAL); ROTAVIRUS;

EID: 79953208274     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02690-10     Document Type: Article

References (19)

1. Álvarez, M. A., M. Herrero, and J. E. Suárez. 1998. The site-specific recombination system of the Lactobacillus species bacteriophage A2 integrates in gram-positive and gram-negative bacteria. Virology 250:185-193.
2. Avall-Jääskeläinen, S., K. Kylä-Nikkilä, M. Kahala, T. Miikkulainen-Lahti, and A. Palva. 2002. Surface display of foreign epitopes on the Lactobacillus brevis S-layer. Appl. Environ. Microbiol. 68:5943-5951.
3. Chancey, C. J., et al. 2006. Lactobacilli-expressed single-chain variable fragment (scFv) specific for intercellular adhesion molecule 1 (ICAM-1) blocks cell-associated HIV-1 transmission across a cervical epithelial monolayer. J. Immunol. 176:5627-5636.
4. Hanniffy, S., et al. 2004. Potential and opportunities for use of recombinant lactic acid bacteria in human health. Adv. Appl. Microbiol. 56:1-64.
5. Hultberg, A., et al. 2007. Lactobacilli expressing llama VHH fragments neutralise Lactococcus phages. BMC Biotechnol. 7:58.
6. Jankovic, I., et al. 2003. Contribution of aggregation-promoting factor to maintenance of cell shape in Lactobacillus gasseri 4B2. J. Bacteriol. 185: 3288-3296.
7. Krüger, C., et al. 2002. In situ delivery of passive immunity by lactobacilli producing single-chain antibody. Nat. Biotechnol. 20:702-706.
8. Krüger, C., et al. 2006. Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries. Appl. Microbiol. Biotechnol. 72:732-737.
9. Marcotte, H., et al. 2004. The aggregation-promoting factor of Lactobacillus crispatus M247 and its genetic locus. J. Appl. Microbiol. 97:749-756.
10. Marcotte, H., et al. 2006. Expression of single-chain antibody against RgpA protease of Porphyromonas gingivalis in Lactobacillus. J. Appl. Microbiol. 100:256-263.
11. Marcotte, H., N. Pant, and L. Hammarström. 2008. Engineered lactobody-producing lactobacilli: a novel form of therapy against rotavirus infection. Future Virol. 3:327-341.
12. Martín, M. C., J. C. Alonso, J. E. Suárez, and M. A. Alvarez. 2000. Generation of food-grade recombinant lactic acid bacterium strains by site-specific recombination. Appl. Environ. Microbiol. 66:2599-2604.
13. Martín, M. C., et al. 2004. Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei. FEMS Microbiol. Lett. 237:385-391.
14. Oggioni, M. R., D. Medaglini, T. Maggi, and G. Pozzi. 1999. Engineering the gram-positive cell surface for construction of bacterial vaccine vectors. Methods 19:163-173.
15. Pant, N., et al. 2006. Lactobacilli expressing variable domain of llama heavychain antibody fragments (lactobodies) confer protection against rotavirusinduced diarrhea. J. Infect. Dis. 194:1580-1588.
16. Pérez-Arellano, I., M. Zúñiga, and G. Pérez-Martínez. 2001. Construction of compatible wide-host-range shuttle vectors for lactic acid bacteria and Escherichia coli. Plasmid 46:106-116.
17. Turner, M. S., L. M. Hafner, T. Walsh, and P. M. Giffard. 2004. Identification and characterization of the novel LysM domain-containing surface protein Sep from Lactobacillus fermentum BR11 and its use as a peptide fusion partner in Lactobacillus and Lactococcus. Appl. Environ. Microbiol. 70: 3673-3680.
18. Ventura, M., I. Jankovic, D. C. Walker, R. D. Pridmore, and R. Zink. 2002. Identification and characterization of novel surface proteins in Lactobacillus johnsonii and Lactobacillus gasseri. Appl. Environ. Microbiol. 68:6172-6181.
19. Wells, J. M., and A. Mercenier. 2008. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat. Rev. Microbiol. 6:349-362.