Quantitative PCR coupled with melt curve analysis for detection of selected Pseudo-nitzschia spp. (Bacillariophyceae) from the northwestern Mediterranean Sea

Applied and Environmental Microbiology

Volumn 77, Issue 5, 2011, Pages 1651-1659

  Andree, Karl B ^a,c   Fernández Tejedor, Margarita ^a,c   Elandaloussi, Laurence M ^a,c   Quijano Scheggia, Sonia ^d   Sampedro, Nagore ^b,c   Garcés, Esther ^b,c   Camp, Jordi ^b,c   Diogène, Jorge ^a,c  

^a IRTA   (Spain)

^b INSTITUT DE CIÈNCIES DEL MAR   (Spain)

^c Generalitat de Catalunya ^*  (Spain)

^d UNIVERSIDAD DE COLIMA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICONS; AS SPECIES; BACILLARIOPHYCEAE; CATALONIA; CELL COUNT; CELL DENSITY; CRYPTIC SPECIES; CURVE ANALYSIS; FALSE POSITIVE; INTERNAL TRANSCRIBED SPACERS; MANAGEMENT TOOL; MOLECULAR METHODS; NORTHWESTERN MEDITERRANEAN SEA; PSEUDO-NITZSCHIA; PSEUDO-NITZSCHIA SPP; QUANTITATIVE PCR; RIBOSOMAL DNA; TOXIGENIC; WATER SAMPLES;

CELL CULTURE; CLONE CELLS; OPTICAL DATA STORAGE; OPTICAL INSTRUMENTS; OPTICAL MICROSCOPY; SCANNING ELECTRON MICROSCOPY; SEAWATER; TOXIC MATERIALS;

POLYMERASE CHAIN REACTION;

RIBOSOMAL SPACER DNA; RIBOSOME DNA; RNA 5.8S; SEA WATER;

DATABASE; DIATOM; ELECTRON MICROSCOPY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SEAWATER; TAXONOMY;

ARTICLE; CHEMISTRY; CLASSIFICATION; CLUSTER ANALYSIS; DIATOM; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; MEDITERRANEAN SEA; METHODOLOGY; MICROBIOLOGY; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA GENE; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY; ULTRASTRUCTURE;

CLUSTER ANALYSIS; DIATOMS; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; GENES, RRNA; MEDITERRANEAN SEA; MICROSCOPY, ELECTRON, SCANNING; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 5.8S; SEAWATER; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ALFACS BAY; CATALONIA; MEDITERRANEAN SEA; MEDITERRANEAN SEA (NORTHWEST); SPAIN; TARRAGONA [CATALONIA];

BACILLARIOPHYCEAE; PSEUDO-NITZSCHIA;

EID: 79953188730     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01978-10     Document Type: Article

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