Titration of fluorochrome-conjugated antibodies for labeling cell surface markers on live cells

Current Protocols in Cytometry

Volumn , Issue SUPPL.54, 2010, Pages

  Hulspas, Ruud ^a  

^a CytonomeST LLC   (United States)

Author keywords

Background; Cell sorting; Flow cytometry; Monoclonal antibodies; Nonspecific binding

Indexed keywords

CELL SURFACE MARKER; FLUOROCHROME; MONOCLONAL ANTIBODY;

ANTIGEN BINDING; ANTIGEN EXPRESSION; ARTICLE; CELL LABELING; CELL SELECTION; CONCENTRATION (PARAMETERS); DATA ANALYSIS; DILUTION; FLOW CYTOMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIGNAL NOISE RATIO; TITRIMETRY;

EID: 79953172890     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0629s54     Document Type: Article

References (4)

1. Hulspas, R., O'Gorman, M.R.G., Wood, B.L., Gratama, J.W., and Sutherland, D.R. 2009. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B 76:355-364.
2. Kantor, A.B. and Roederer, M. 1996. FACS analysis of leukocytes. In Handbook of Experimental Immunology, 5th ed. (L.A. Herzenberg, D.M. Weir, and C. Blackwell, eds.) pp. 49.1-49.13. Blackwell Scientific, Cambridge, Mass.
3. Owens, M.A. and Loken, M.R. 1997. Titering antibodies. In Flow cytometry principles for clinical laboratory practice, pp. 65-67. Wiley-Liss, New York.
4. Srivastava, P., Sladek, T.L., Goodman, M.N., and Jacobberger, J.W. 1992. Streptavidin based quantitative staining of intracellular antigens for flow cytometric analysis. Cytometry 13:711-721.