Heterogeneity of Aspergillus niger microcolonies in liquid shaken cultures

Applied and Environmental Microbiology

Volumn 77, Issue 4, 2011, Pages 1263-1267

  de Bekker, Charissa ^a   van Veluw, G Jerre ^a   Vinck, Arman ^a   Wiebenga, L Ad ^a   Wösten, Han A B ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS NIGER; COMPLEX OBJECTS; FERULIC ACID ESTERASE; GREEN FLUORESCENT PROTEIN; INDUSTRIAL FERMENTATION; LASER MICRODISSECTIONS; LOW LEVEL; MICROCOLONIES; NIGER; PROTEIN PRODUCTION; QUANTITATIVE PCR; SHAKEN CULTURE; STATISTICAL ANALYSIS;

ASPERGILLUS; FLUORESCENCE; GENE EXPRESSION; INDUSTRIAL WASTES; LIQUIDS; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; RNA;

POPULATION STATISTICS;

CARBOXYLESTERASE; FERULOYL ESTERASE; GLUCAN 1,4 ALPHA GLUCOSIDASE; GREEN FLUORESCENT PROTEIN; RNA;

COLONY; FERMENTATION; FUNGUS; GENE EXPRESSION; HETEROGENEITY; PROTEIN; QUANTITATIVE ANALYSIS;

ARTICLE; ASPERGILLUS NIGER; BIOREACTOR; CLASSIFICATION; CYTOLOGY; FERMENTATION; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; MYCELIUM; POLYMERASE CHAIN REACTION; PROMOTER REGION; ULTRASTRUCTURE;

ASPERGILLUS NIGER; BIOREACTORS; CARBOXYLIC ESTER HYDROLASES; FERMENTATION; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION REGULATION, FUNGAL; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GREEN FLUORESCENT PROTEINS; MYCELIUM; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RNA;

ASPERGILLUS NIGER; FUNGI;

EID: 79953170251     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02134-10     Document Type: Article

References (20)

1. Boel, E., et al. 1984. Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs. EMBO J. 3:1097-1102.
2. Bos, C. J., et al. 1988. Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr. Genet. 14:437-443.
3. Conesa, A., P. J. Punt, N. van Luijk, and C. A. M. J. J. van den Hondel. 2001. The secretion pathway in filamentous fungi: a biotechnological view. Fungal Genet. Biol. 33:155-171.
4. de Vries, R. P., and J. Visser. 1999. Regulation of the feruloyl esterase (faeA) gene from Aspergillus niger. Appl. Environ. Microbiol. 65:5500-5503.
5. El-Enshasy, H., J. Kleine, and U. Rinas. 2006. Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger. Process Biochem. 41:2103-2112.
6. Finkelstein, D. B., et al. 1989. Protein secretion in Aspergillus niger, p. 295-300. In C. L. Hershberger, S. W. Queener, and G. Hegeman (ed.), Genetics and molecular biology of industrial microorganisms. American Society for Microbiology, Washington, DC.
7. Freudenberg, S., K. Fasold, S. R. Müller, D. Siedenberg, G. Kretzmer, K. Schügerl, and M. Giuseppin. 1996. Fluorescence microscopic investigation of Aspergillus awamori growing on synthetic and complex media and producing xylanase. J. Biotechnol. 46:265-273.
8. Goosen, T., et al. 1987. Transformation of Aspergillus niger using the homologous orotidine-5_-phosphate-decarboxylase gene. Curr. Genet. 11:499-503.
9. Jennings, D. H., J. D. Thornton, M. F. Galpin, and C. R. Coggins. 1974. Translocation in fungi. Symp. Soc. Exp. Biol. 28:139-156.
10. Kasuga, T., and N. L. Glass. 2008. Dissecting colony development of Neurospora crassa using mRNA profiling and comparative genomics approaches. Eukaryot. Cell 7:1549-1564.
11. Kusters-van Someren, M. A., J. A. M. Harmsen, H. C. M. Kester, and J. Visser. 1991. Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans. Curr. Genet. 20:293-299.
12. Levin, A. M., et al. 2007. Spatial differentiation in the vegetative mycelium of Aspergillus niger. Eukaryot. Cell 6:2311-2322.
13. Masai, K., et al. 2006. Square-plate culture method allows detection of differential gene expression and screening of novel, region-specific genes in Aspergillus oryzae. Appl. Microbiol. Biotechnol. 71:881-891.
14. Moukha, S. M., H. A. B. Wösten, E. Mylius, M. Asther, and J. G. H. Wessels. 1993. Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium. J. Bacteriol. 175:3672-3678.
15. Petersen, K. L., J. Lehmbeck, and T. Christensen. 1999. A new transcriptional activator for amylase genes in Aspergillus. Mol. Gen. Genet. 262:668-676.
16. Punt, P. J., et al. 2002. Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol. 20:200-206.
17. Siedenberg, D., et al. 1999. GlaA promoter controlled production of a mutant green fluorescent protein (S65T) by recombinant Aspergillus niger during growth on defined medium in batch and fed-batch cultures. Biotechnol. Prog. 15:43-50.
18. Vinck, A., et al. 16 August 2010, posting date. Heterogenic expression of genes encoding secreted proteins at the periphery of Aspergillus niger colonies. Environ. Microbiol. doi:10.1111/j.1462-2920.2010.02322.x.
19. Vinck, A., et al. 2005. Hyphal differentiation in the exploring mycelium of Aspergillus niger. Mol. Microbiol. 58:693-699.
20. Wösten, H. A. B., S. M. Moukha, J. H. Sietsma, and J. G. H. Wessels. 1991. Localization of growth and secretion of proteins in Aspergillus niger. J. Gen. Microbiol. 137:2017-2023.