Glycol Methacrylate Embedding for Improved Morphological, Morphometrical, and Immunohistochemical Investigations Under Light Microscopy: Testes as a Model

Methods in Molecular Biology

Volumn 689, Issue , 2011, Pages 3-18

  Chiarini Garcia, Hélio ^a   Parreira, Gleydes Gambogi ^a   Almeida, Fernanda R C L ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

embedding; fixation; Glycol methacrylate; light microscopy; testes

Indexed keywords

2 HYDROXYETHYL METHACRYLATE; DIAGNOSTIC AGENT; METHACRYLIC ACID DERIVATIVE;

ANIMAL; ARTICLE; CHEMISTRY; EVALUATION; HISTOLOGY; IMMUNOHISTOCHEMISTRY; MALE; MAMMAL; METHODOLOGY; MICROSCOPY; TESTIS; TISSUE FIXATION; ULTRASTRUCTURE;

ANIMALS; IMMUNOHISTOCHEMISTRY; MALE; MAMMALS; METHACRYLATES; MICROSCOPY; TESTIS; TISSUE EMBEDDING; TISSUE FIXATION;

MLCS; MLOWN;

EID: 79953139014     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-950-5_1     Document Type: Chapter

References (30)

1. Clermont, Y., Perey, B. (1957) Quantitative study of the cell population of the seminiferous tubules of immature rats. Am J Anat 100, 241–268.
2. Clermont, Y., Perey, B. (1957) The stages of the cycle of the seminiferous epithelium of the rat: practical definitions in PA-Schiff-hematoxylin stained sections. Rev Can Biol 16, 451–462.
3. Clermont, Y. (1962) Quantitative analysis of spermatogenesis of the rat: a revised model for the renewal of spermatogonia. Am J Anat 111, 111–129.
4. Clermont, Y., Bustos-Obregon, E. (1968) Re-examinations spermatogonial renewal in the rat by means of seminiferous tubules mounted ‘in toto’. Am J Anat 122, 237–247.
5. Russell, L. D., Clermont, Y. (1977) Degeneration of germ cells in normal, hypophysec- tomized and hormone treated hypophysec-tomized rats. Anat Rec 187, 347–366.
6. Chiarini-Garcia, H., Russell, L. D. (2001) High-resolution light microscopic characterization of mouse spermatogonia. Biol Reprod 65, 1170–1178.
7. Chiarini-Garcia, H., Hornick, J. R., Gris-wold, M. D., Russell, L. D. (2001) Distribution of type-A spermatogonia in the mouse is not random. Biol Reprod 65, 1179–1185.
8. Russell, L. D., Chiarini-Garcia, H., Korsmeyer, S. J., Knudson, C. M. (2002) Bax-dependent spermatogonia apopto-sis is required for testicular development and spermatogenesis. Biol Reprod 66, 950–958.
9. Chiarini-Garcia, H., Raymer, A. M., Russell, L. D. (2003) Non-random distribution of spermatogonia in rats: evidence of niches in the seminiferous tubules. Reproduction 126, 669–680.
10. jsd ) mice. Biol Reprod 77, 237–246.
11. Nascimento, H. F., Drumond, A. L., França, L. R., Chiarini-Garcia, H. (2008) Spermatogonial morphology, kinetics and niches in hamsters exposed to short-and long-photoperiod. Int J Androl, 32, 486–497. Doi:10.1111/j.1365–2605.2008.00884.x.
12. Chiarini-Garcia, H., Meistrich, M. L. (2008) High-resolution light microscopic characterization of spermatogonia, in (Hou, S. X., Singh, S. R., eds.), Germline Stem Cells, vol 450. Humana Press, Totowa, NJ, Methods in molecular biology, pp. 95–107.
13. Chiarini-Garcia, H., Alves-Freitas, D., Barbosa, I. S., Almeida, F. R. L. C. (2009) Evaluation of the seminiferous epithelial cycle, spermatogonial kinetics and niche in donkeys (Equus asi-nus). Anim Reprod Sci, 116, 139–154. Doi:10.1016/j.anireprosci.2008.12.019.
14. Bennett, H. S., Wyrick, A. D., Lee, S. W., McNeil, J. H. (1976) Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains. Stain Technol 51, 71–97.
15. Cole, M. B., Jr., Sykes, S. M. (1974) Glycol methacrylate in microscopy: a routine method for embedding and sectioning animal tissues. Stain Technol 49, 387–400.
16. Woodruff, J. M., Greenfield, S. A. (1979) Advantages of glycol methacrylate embedding systems for light microscopy. J Histotechnol 2, 164–167.
17. Chiarini-Garcia, H., Machado, C. R. S. (1992) Mast cell types in the lymph nodes of the opossum Didelphis albiventris (Mar-supialia, Didelphidae). Cell Tissue Res 268, 571–574.
18. Chiarini-Garcia, H., Ferreira, R. M. A. (1992) Histochemical evidence of heparin in granular cells of Hoplias malabaricus Bloch. J Fish Biol 41, 155–157.
19. Chiarini-Garcia, H., Pereira, F. M. A. (1999) Comparative studies of lymph nodes mast cell populations form five different marsupials species. Tissue Cell 31, 318–326.
20. Chiarini-Garcia, H., Santos, A. A. D., Machado, C. R. S. (2000) Mast cell types and cell-to-cell interactions in lymph nodes of the opossum Didelphis albiventris. Anat Embryol 201, 197–206.
21. Santos, A. A. D., Chiarini-Garcia, H., Oliveira, K. R., Machado, C. R. S. (2003) Development of different mast cell types in the opossum Didelphis albiventris. Anat Embryol 206, 239–245.
22. Rocha, J. S., Chiarini-Garcia, H. (2007) Mast cell heterogeneity between two different species of Hoplias sp (Characiformes: Erythrinidae): response to fixatives, anatomical distribution, histochemical contents and ultrastructural features. Fish Shellfish Immun 22, 218–229.
23. Paula, T. A. R., Chiarini-Garcia, H., França, L. R. (1999) Seminiferous epithelium cycle and its duration in capybaras (Hydrochoerus hydrochaeris). Tissue Cell 31, 327–334.
24. Neves, E. S., Chiarini-Garcia, H., França, L. R. (2002) Comparative testis morphometry and seminiferous epithelium cycle length in donkey and mules. Biol Reprod 67, 247–255.
25. Amaral, D., Chiarini-Garcia, H., Vale Filho, V. R., Allen, W. R. (2004) Effects of formalin and bouin fixation upon the mare’s endome-trial biopsies embedded in plastic resin. Braz J Vet Anim Sci 56, 7–12.
26. Melo, R. C. N., Rosa, P. G., Noyma, N. P., Pereira, W. F., Tavares, L. E. R., Parreira, G. G., Chiarini-Garcia, H., Roland, F. (2007) Histological approaches for high-quality imaging of zooplanktonic organisms. Micron (Oxford) 38, 714–721.
27. Bozzola, J. J., Russell, L. D. (1999) Electron Microscopy: Principles and Techniques for Biologists, 2nd edn. Jones and Bartlett, Sudbury, MA.
28. Karnovsky, M. J. A. (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27, 137A–138A.
29. Shuttlesworth, G. A., de Rooij, D.G., Huhtaniemi, I., Reissmann, T., Russell, L.D., Shetty, G., Wilson, G., Meistrich, M.L.. (2000) Enhancement of a spermatogonial proliferation and differentiation in irradiated rats by gonadotropin-releasing hormone antagonist administration. Endocrinol 141, 37–49.
30. Russell, L. D., Ettlin, R.A., Sinha Hikim, A. P., Clegg, E. D. (eds.) (1990) Histological and Histopathological Evaluation of the Testis. Cache River Press, Clearwater, IL.