Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infection

Methods in Molecular Biology

Volumn 689, Issue , 2011, Pages 69-80

  Fabrino, Daniela L ^a   Ribeiro, Grazielle A ^a   Teixeira, Lívia ^a   Melo, Rossana C N ^a  

^a FEDERAL UNIVERSITY OF JUIZ DE FORA   (Brazil)

Author keywords

animal models; Chagas disease; glycol methacrylate; heart; histology; inflammation; Trypanosoma cruzi

Indexed keywords

2 HYDROXYETHYL METHACRYLATE; EOSIN; FUCHSINE; HEMATOXYLIN; METHACRYLIC ACID DERIVATIVE; TOLONIUM CHLORIDE;

ANIMAL; ARTICLE; HEART; HISTOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROTOMY; PARASITOLOGY; RAT; TISSUE FIXATION; TRYPANOSOMA CRUZI; ULTRASTRUCTURE;

ANIMALS; EOSINE YELLOWISH-(YS); HEART; HEMATOXYLIN; HISTOLOGICAL TECHNIQUES; METHACRYLATES; MICROTOMY; RATS; ROSANILINE DYES; TISSUE EMBEDDING; TISSUE FIXATION; TOLONIUM CHLORIDE; TRYPANOSOMA CRUZI;

MLCS; MLOWN;

EID: 79953127420     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-950-5_5     Document Type: Chapter

References (15)

1. Chagas, C. (1909) New human trypanoso-miasis. Morphology and life cycle of Schysotrypanum cruzi, the course of a new human disease. Mem I Oswaldo Cruz 1, 159–218.
2. Melo, R. C. N. (2009) Acute heart inflammation: ultrastructural and functional aspects of macrophages elicited by Trypanosoma cruzi infection. J Cell Mol Med 13, 279–294.
3. Teixeira, A. R., Nitz, N., Guimaro, M. C., Gomes, C., Santos-Buch, C. A. (2006) Cha-gas’ disease. Postgrad Med J 82, 788–798.
4. Ropert, C., Ferreira, L. R., Campos, M. A., Procopio, D. O., Travassos, L. R., Ferguson, M. A., Reis, L. F., Teixeira, M. M., Almeida, I. C., Gazzinelli, R. T. (2002) Macrophage signaling by glycosylphosphatidylinositol-anchored mucin-like glycoproteins derived from Trypanosoma cruzi trypomastigotes. Microbes Infect 4, 1015–1025.
5. Teixeira, A. R., Nascimento, R. J, Sturm, N. R. (2006) Evolution and pathology in Cha-gas’ disease – a review. Mem I Oswaldo Cruz 101, 463–491.
6. Melo, R. C. N., Rosa, P. G., Noyma, N. P., Pereira, W. F., Tavares, L. E., Parreira, G. G., Chiarini-Garcia, H., Roland, F. (2007) Histological approaches for high-quality imaging of zooplanktonic organisms. Micron 38, 714–721.
7. Bennett, H. S., Wyrick, A. D., Lee, S. W., McNeil, J. H. (1976) Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains. Stain Technol 51, 71–97.
8. Cole, M. B., Jr., Sykes, S. M. (1974) Glycol methacrylate in light microscopy: a routine method for embedding and sectioning animal tissues. Stain Technol 49, 387–400.
9. Hanson, W. L., Roberson, E. L. (1974) Density of parasites in various organs and the relation to numbers of trypomastigotes in the blood during acute infections of Trypanosoma cruzi in mice. J Protozool 21, 512–517.
10. Melo, R. C. N., Machado, C. R. S. (2001) Trypanosoma cruzi: peripheral blood monocytes and heart macrophages in the resistance to acute experimental infection in rats. Exp Parasitol 97, 15–23.
11. Cerri, P. S., Sasso-Cerri, E. (2003) Staining methods applied to glycol methacrylate embedded tissue sections. Micron 34, 365–372.
12. Fabrino, D. L., Leon, L. L., Parreira, G. G., Genestra, M., Almeida, P. E., Melo, R. C. N. (2004) Peripheral blood monocytes show morphological pattern of activation and decreased nitric oxide production during acute Chagas’ disease in rats. Nitric Oxide 11, 166–174.
13. Abreu, M., Baroza, L., Rossi, M. (1993) Toluidine blue-basic fuchsin stain for glycol-methacrylate embedded tissue. J Histotechnol 16, 139–140.
14. Melo, R. C. N., Machado, C. R. S. (1998) Depletion of radiosensitive leukocytes exacerbates the heart sympathetic den-ervation and parasitism in experimental Cha-gas’ disease in rats. J Neuroimmunol 84, 151–157.
15. Mann, H. B., Whitney, D. R. (1947) On a test of whether one of two random variables is stochastically larger than the other. Ann Math Statistics 18, 50–60.