The use of an in vitro microneutralization assay to evaluate the potential of recombinant VP5 protein as an antigen for vaccinating against Grass carp reovirus

Virology Journal

Volumn 8, Issue , 2011, Pages

  He, Yongxing ^a   Xu, Hongxu ^b   Yang, Qian ^a   Xu, Dan ^a   Lu, Liqun ^a  

^a SHANGHAI OCEAN UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE; GLUTATHIONE VP5 PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; RECOMBINANT VP5 PROTEIN; SUBUNIT VACCINE; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS PROTEIN; VIRUS ANTIGEN; VIRUS VACCINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE ISOLATION; GENETIC ENGINEERING; GRASS CARP REOVIRUS; GRASS CARP REOVIRUS INFECTION; IN VITRO STUDY; MOLECULAR WEIGHT; NONHUMAN; PROGENY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REOVIRUS; REOVIRUS INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERODIAGNOSIS; VIRION; VIRUS GENOME; VIRUS NEUTRALIZATION; VIRUS TITRATION; WESTERN BLOTTING; ANIMAL; ANIMAL DISEASE; BAGG ALBINO MOUSE; CELL LINE; CYPRINIFORMES; FEMALE; FISH DISEASE; GENETICS; IMMUNOLOGY; MOUSE; VACCINATION; VIROLOGY;

CTENOPHARYNGODON; GRASS CARP REOVIRUS; MUS;

ANIMALS; ANTIGENS, VIRAL; CELL LINE; CYPRINIDAE; FEMALE; FISH DISEASES; MICE; MICE, INBRED BALB C; NEUTRALIZATION TESTS; RECOMBINANT PROTEINS; REOVIRIDAE; REOVIRIDAE INFECTIONS; VACCINATION; VIRAL PROTEINS; VIRAL VACCINES;

EID: 79953113366     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-8-132     Document Type: Article

References (23)

1. Qiu T, Lu R, Zhang J, Zhu Z: Complete nucleotide sequence of the S10 genome segment of grass carp reovirus (GCRV). Dis Aquat Org 2001, 44:69-74. (Pubitemid 32165869)
2. Zhang C, Wang Q, Shi C, Zeng W, Liu Y, Wu S: Molecular analysis of grass carp reovirus HZ08 genome segments 1-3 and 5-6. Virus Genes 2010, 41(1):102-104.
3. Francki RIB, Fauquet CM, Knudson DL, Brown F: Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses Wien: Springer-Verlag; 1991.
4. Attoui H, Fang Q, Jaafar FM, Cantaloube J, Biagini P, Philippe dM, Lamballerie X: Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae). J Gen Virol 2002, 83:1941-1951. (Pubitemid 34846457)
5. Tyler KL: Pathogenesis of reovirus infections of the central nervous system. Seminars in Neuroscience 1991, 3(2):117-124.
6. Van der Heide L, Kalbac M, Brustolon M: Development of an attenuated apathogenic reovirus vaccine against viral arthritis/Tenosynovitis. Avian Dis 1983, 27(3):698-706.
7. Guo ZY, Giambrone JJ, Wu H, Dormitorio T: Safety and Efficacy of an Experimental Reovirus Vaccine for In Ovo Administration. Avian Dis 2003, 47(4):1423-1428. (Pubitemid 37538169)
8. Vasserman Y, Eliahoo D, Hemsani E, Kass N, Ayali G, Pokamunski S, Pitcovski J: The Influence of Reovirus Sigma C Protein Diversity on Vaccination Efficiency. Avian Dis 2004, 48(2):271-278. (Pubitemid 39508864)
9. Sommerset I, Krossøy B, Biering E, Frost P: Vaccines for fish in aquaculture. Expert rev vaccines 2005, 4(1):89-101. (Pubitemid 40327414)
10. Zeng L, Yang X, He L, Ai X, He J, Zuo W: Batch processing technology of cell cultured killed vaccine against the hemorrhage of Grass carp. J Fishery Sci China 1998, 5(2):62-67.
11. Ellis AE: Immunity to bacteria in fish. Fish Shell Immunol 1999, 9(4):291-308.
12. Cheng L, Zhu J, Hui WH, Zhang X, Honig B, Fang Q, Zhou ZH: Backbone Model of an Aquareovirus Virion by Cryo-Electron Microscopy and Bioinformatics. J Mol Biol 2009, 397(2):852-863.
13. Cheng L, Fang Q, Shah S, Atanasov IC, Zhou ZH: Subnanometer-Resolution Structures of the Grass Carp Reovirus Core and Virion. J Mol Biol 2008, 382(1):213-222.
14. Chandran K, Walker SB, Chen Y, Contreras CM, Schiff LA, Baker TS, Nibert ML: In vitro recoating of reovirus cores with baculovirus-expressed outer-capsid proteins μ1 and 3. J Virol 1999, 73:3941-3950.
15. Fan C, Shao L, Fang Q: Characterization of the nonstructural protein NS80 of grass carp reovirus. Arch Virol 2010, 155(11):1755-1763.
16. Andreoni M, Faircloth M, Vugler L, Britt W: A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus. J Virol Meth 1989, 23(2):157-167. (Pubitemid 19069993)
17. Pastrana DV, Vass WC, Lowy DR, Schiller JT: NHPV16 VLP Vaccine Induces Human Antibodies That Neutralize Divergent Variants of HPV16. Virology 2000, 279(1):361-369. (Pubitemid 34161026)
18. Schofield DJ, Glamann J, Emerson SU, Purcel RH: Identification by Phage Display and Characterization of Two Neutralizing Chimpanzee Monoclonal Antibodies to the Hepatitis E Virus Capsid Protein. J Virol 2000, 74(12):5548-5555. (Pubitemid 30346588)
19. Rangel AA, Rockemann DD, Hetrick FM, Samal SK: Identification of grass carp haemorrhage virus as a new genogroup of aquareovirus. J Gen Virol 1999, 80:2399-2402. (Pubitemid 29408367)
20. Fang Q, Seng EK, Ding QQ, Zhang LL: Characterization of infectious particles of grass carp reovirus by treatment with proteases. Arch Virol 2008, 153(4):675-682.
21. Zuo WG, Qian HX, Xue YF: Establishment of grass carp kidney cell line (CIK), Fresh-water Fisheries. 1984, , 2: 38-39, (in Chinese).
22. LaBarre DD, Lowy RJ: Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays. J Virol Methods 2001, 96(2):107-126. (Pubitemid 32607532)
23. Crawford-Miksza LK, Schnurr DP: Quantitative colorimetric microneutralization assay for characterization of Adenovirus. J Clin Microbiol 1994, 32(9):2331-2334. (Pubitemid 24258497)