Evaluation of three commercially available real-time PCR based systems for detection of Cronobacter species

International Journal of Food Microbiology

Volumn 146, Issue 2, 2011, Pages 200-202

  Fricker Feer, C ^a   Cernela, N ^b   Bolzan, S ^a   Lehner, A ^b   Stephan, R ^a  

^a QA and Food Safety Department Hochdorf Nutritec AG   (Switzerland)

^b VETSUISSE FACULTY UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Commercial real time PCR systems evaluation; Cronobacter spp.; Detection

Indexed keywords

ARTICLE; ARTIFICIAL MILK; BACTERIUM CULTURE; BACTERIUM DETECTION; COMMERCIAL PHENOMENA; CONTROLLED STUDY; CRONOBACTER; DIAGNOSTIC PROCEDURE; ENTEROBACTER SAKAZAKII; INTERMETHOD COMPARISON; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; SPECIES IDENTIFICATION;

ENTEROBACTER SAKAZAKII; FOOD CONTAMINATION; HUMANS; INFANT; INFANT FORMULA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ENTEROBACTER SAKAZAKII;

EID: 79953094722     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.02.006     Document Type: Article

References (14)

1. Anonymous Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. OJ L 338, 22.12.2005 2005, 1-26.
2. Hunter C.J., Petrosyan M., Ford H.R., Prasadarao N.V. Enterobacter sakazakii: an emerging pathogen in infants and neonates. Surgical Infections 2008, 9:533-539.
3. Iversen C., Lehner A., Mullane N., Marugg J., Fanning S., Stephan R., Joosten H. Identification of "Cronobacter" spp. (Enterobacter sakazakii). Journal of Clinical Microbiology 2007, 45:3814-3816.
4. Iversen C., Mullane N., Mc Cardell B., Tall B., Lehner A., Fanning S., Stephan R., Joosten J. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov. comb. nov., C. malonaticus sp. nov., C. turicensis sp. nov., C. muytjensii sp. nov., C. dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, C. dublinensis sp. nov. subsp. dublinensis subsp. nov., C. dublinensis sp. nov. subsp. lausannensis subsp. nov., and C. dublinensis sp. nov. subsp. lactaridi subsp. nov. International Journal of Systematic and Evolutionary Microbiology 2008, 58:1442-1447.
5. Iversen C., Druggan P., Schumacher S., Lehner A., Feer C., Gschwend K., Joosten H., Stephan R. Development of a novel screening method for the isolation of Cronobacter spp. (Enterobacter sakazakii). Applied and Environmental Microbiology 2008, 74:2550-2553.
6. Kuhnert P., Korczak B.M., Stephan R., Joosten H., Iversen C. Phylogeny and whole genome DNA-DNA similarity of Cronobacter (Enterobacter sakazakii) and related species by multilocus sequence analysis (MLSA). International Journal of Food Microbiology 2009, 136:152-158.
7. Lehner A., Stephan R. Microbiological, epidemiological and food safety aspects of Enterobacter sakazakii. Journal of Food Protection 2004, 67:2850-2857.
8. Lehner A., Nitzsche S., Breeuwer P., Diep B., Thelen K., Stephan R. Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection. BMC Microbiology 2006, 6:15.
9. Mohan Nair M.K., Venkitanarayanan K.S. Cloning and sequencing of the ompA gene of Enterobacter sakazakii and development of an ompA-targeted PCR for rapid detection of Enterobacter sakazakii in infant formula. Applied and Environmental Microbiology 2006, 72:2539-2546.
10. Popp A., Iversen C., Fricker-Feer C., Gschwend K., Stephan R. Identification of Enterobacteriaceae isolates from raw ingredients, environmental samples and products of an infant formula processing plant. Archiv für Lebensmittelhygiene 2009, 60:92-97.
11. Stephan R., Ziegler D., Pflüger V., Vogel G., Lehner A. Rapid genus and species specific identification of Cronobacter spp. by MALDI-TOF Mass spectrometry. Journal of Clinical Microbiology 2010, 48:2846-2851.
12. Stoop B., Lehner A., Iversen C., Fanning C., Stephan R. Development and evaluation of rpoB based PCR systems to differentiate the six proposed species within the genus Cronobacter. International Journal of Food Microbiology 2009, 136:165-168.
13. van Acker J., de Smet F., Muyldermans G., Bougatef A., Naessens A., Lauwers S. Outbreak of necrotizing enterocolitis associated with Enterobacter sakazakii in powdered milk formula. Journal of Clinical Microbiology 2001, 39:293-297.
14. Weir E. Powdered infant formula and fatal infection with Enterobacter sakazakii. Canadian Medical Association Journal 2002, 166:1570.