A candidate inactivated chimeric vaccine PCV1-2 constructed based on PCV1 and PCV2 isolates originating in China and its evaluation in conventional pigs in regard to protective efficacy against PCV2 infection

Microbiology and Immunology

Volumn 55, Issue 4, 2011, Pages 254-266

  Xujie, Liu ^a   Xiaobo, Wang ^a   Yi, Song ^a   Jing, Fan ^a   Song, Gao ^a   Xiufan, Liu ^a  

^a YANGZHOU UNIVERSITY   (China)

Author keywords

Candidate inactivated vaccine; Chimeric pCV1 2; Protective immunity

Indexed keywords

INACTIVATED VIRUS VACCINE; INACTIVATED VIRUS VACCINE PCV 1-2; MATERNAL ANTIBODY; NEUTRALIZING ANTIBODY; UNCLASSIFIED DRUG; VIRUS ANTIGEN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY TITER; ARTICLE; CHINA; CIRCOVIRIDAE INFECTION; CIRCOVIRUS; CIRCOVIRUS 1; CIRCOVIRUS 2; CLINICAL EVALUATION; CONTROLLED STUDY; DNA VIRUS; DRUG EFFICACY; DRUG SYNTHESIS; IMMUNOFLUORESCENCE TEST; IMMUNOGENICITY; LUNG LESION; LYMPH NODE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; SEROCONVERSION; SWINE; VIRUS CAPSID; VIRUS GENOME; VIRUS IMMUNITY; VIRUS ISOLATION; VIRUS LOAD; VIRUS NEUTRALIZATION; VIRUS STRAIN; WILD TYPE;

ANIMALS; ANTIBODIES, VIRAL; CAPSID PROTEINS; CELL LINE; CHINA; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; SWINE; SWINE DISEASES; VACCINES, INACTIVATED; VIRAL VACCINES;

MIRIDAE; SUIDAE;

EID: 79953080746     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2011.00311.x     Document Type: Article

References (30)

1. Tischer I., Gelderblom H., Vettermann W., Koch M.A. (1982) A very small porcine virus with circular single-stranded DNA. Nature 295: 64-66.
2. Tischer I., Rasch R., Tochtermann G. (1974) Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol [Orig A] 226: 153-67.
3. Cheung A.K. (2003) Transcriptional analysis of porcine circovirus type 2. Virology 305: 168-80.
4. Fenaux M., Halbur P.G., Gill M., Toth T.E., Meng X.J. (2000) Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38: 2494-503.
5. Mankertz A., Domingo M., Folch J.M., Lecann P., Jestin A., Segales J., Chmielewicz B., Plana-Duran J., Soike D. (2000) Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Res 66: 65-77.
6. Nawagitgul P., Morozov I., Bolin S.R., Harms P.A., Sorden S.D., Paul P.S. (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81: 2281-7.
7. Allan G.M., Kennedy S., Mcneilly F., Foster J.C., Ellis J.A., Krakowka S.J., Meehan B.M., Adair B.M. (1999) Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121: 1-11.
8. Allan G.M., Mcneilly F., Kennedy S., Daft B., Clarke E.G., Ellis J.A., Haines D.M., Meehan B.M., Adair B.M. (1998) Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10: 3-10.
9. Ellis J.A., Bratanich A., Clark E.G., Allan G., Meehan B., Haines D.M., Harding J., West K.H., Krakowka S., Konoby C., Hassard L., Martin K., Mcneilly F. (2000) Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12: 21-7.
10. Harms P.A., Sorden S.D., Halbur P.G., Bolin S.R., Lager K.M., Morozov I., Paul P.S. (2001) Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38: 528-39.
11. Ladekjaer-Mikkelsen A.S., Nielsen J., Stadejek T., Storgaard T., Krakowka S., Ellis J., Mcneilly F., Allan G., Botner A. (2002) Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Vet Microbiol 89: 97-114.
12. Opriessnig T., Fenaux M., Yu S., Evans R.B., Cavanaugh D., Gallup J.M., Pallares F.J., Thacker E.L., Lager K.M., Meng X.J., Halbur P.G. (2004) Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus. Vet Microbiol 98: 209-20.
13. Segales J., Allan G.M., Domingo M. (2005) Porcine circovirus diseases. Anim Health Res Rev 6: 119-42.
14. Fort M., Sibila M., Perez-Martin E., Nofrarias M., Mateu E., Segales J. (2009) One dose of a porcine circovirus 2 (PCV2) sub-unit vaccine administered to 3-week-old conventional piglets elicits cell-mediated immunity and significantly reduces PCV2 viremia in an experimental model. Vaccine 27: 4031-7.
15. Segales J., Urniza A., Alegre A., Bru T., Crisci E., Nofrarias M., Lopez-Soria S., Balasch M., Sibila M., Xu Z., Chu H.J., Fraile L., Plana-Duran J. (2009) A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms. Vaccine 27: 7313-21.
16. Reynaud G., Brun A., Charreyre C., Desgouilles S., Jeannin P. (2004) Safety of a repeated overdose of an inactivated adjuvanted PCV2 vaccine in conventional pregnant filts and sows. Proceedings of the 18th Congress International Pigs Veterinary Society; June 18-July 1 2004, Hamburg, Germany
17. Fenaux M., Opriessnig T., Halbur P.G., Elvinger F., Meng X.J. (2004) A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol 78: 6297-303.
18. Wang H.Y., Liu W.B., Gao S., Liu X.F. (2008) Inhibition of porcine circovirus type 2 replication by the plasmid vector expressing siRNAs. Acta Microbiologica Sinica 48: 1507-13.
19. Fenaux M., Opriessnig T., Halbur P.G., Meng X.J. (2003) Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. J Virol 77: 11232-43.
20. Sambrook J., Russell D.W. (2001) Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. In: Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, pp. 463-70.
21. Reed L.J., Muench H. (1938) A simple method of estimating fifty percent endpoints. The American Journal of Hygiene 27: 493-97.
22. Sorden S.D., Harms P.A., Nawagitgul P., Cavanaugh D., Paul P.S. (1999) Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest 11: 528-30.
23. Xu H., Gao S. (2010) Characterization and potential application of monoclonal antibodies against the capsid protein of porcine circovirus type 2, to be submitted.
24. Yin Z., Liu J. (1997) Immunity-serum test, neutralization experiment. In: Yin Z., Liu J., eds. Animal Virology. Beijing: Chinese Science Press, pp. 336-40.
25. Fenaux M., Halbur P.G., Haqshenas G., Royer R., Thomas P., Nawagitgul P., Gill M., Toth T.E., Meng X.J. (2002) Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76: 541-51.
26. Opriessnig T., Madson D.M., Prickett J.R., Kuhar D., Lunney J.K., Elsener J. Halbur P.G. (2008) Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection. Vet Microbiol 131: 103-14.
27. Allan G.M., Ellis J.A. (2000) Porcine circoviruses: a review. J Vet Diagn Invest 12: 3-14.
28. Ellis J., Krakowka S., Lairmore M., Haines D., Bratanich A., Clark E., Allan G., Konoby C., Hassard L., Meehan B., Martin K., Harding J., Kennedy S., Mcneilly F. (1999) Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J Vet Diagn Invest 11: 3-14.
29. Kennedy S., Moffett D., Mcneilly F., Meehan B., Ellis J., Krakowka S., Allan G.M. (2000) Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus. J Comp Pathol 122: 9-24.
30. Fort M., Fernandes L.T., Nofrarias M., Diaz I., Sibila M., Pujols J., Mateu E., Segales J. (2009) Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets. Vet Immunol Immunopathol 129: 101-7.