Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure

Animal Reproduction Science

Volumn 124, Issue 1-2, 2011, Pages 85-89

  Gutierrez Cepeda L ^a   Fernandez A ^a   Crespo, F ^b   Gosalvez J ^c   Serres, C ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b Centro Militar de Cría Caballar FESCCR Ministry of Defense   (Spain)

^c UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Colloidal centrifugation; Equine semen processing; EquiPure

Indexed keywords

ANIMAL; ANIMAL DISEASE; ARTICLE; CENTRIFUGATION; CLINICAL PROTOCOL; ECONOMICS; EVALUATION; HORSE; LABORATORY DIAGNOSIS; MALE; METHODOLOGY; SEMEN ANALYSIS; SPERM RETRIEVAL;

ANIMALS; CENTRIFUGATION; CLINICAL PROTOCOLS; HORSES; MALE; SEMEN ANALYSIS; SPECIMEN HANDLING; SPERM RETRIEVAL;

EQUIDAE;

EID: 79953028160     PISSN: 03784320     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anireprosci.2011.02.001     Document Type: Article

References (24)

1. Alvarenga M.A., Leao K.M., Papa F.O., Landim-Alvarenga F.C., Medeiros A.S.L., Gomes G.M. The use of alternative cryoprotectors for freezing stallion semen. Proceedings, Workshop on Transporting Gametes and Embryos 2003, 74-76.
2. Aurich C. Recent advances in cooled-semen technology. Anim. Reprod. Sci. 2008, 107:268-275.
3. Benito D., Alvarez A., Crespo F., Mateos E., Gómez-Cuétara C., Serres C. Análisis computerizado del semen de caballo de Pura Raza Española. IV Congreso Ibérico de Reproducción Animal 2003, Las Palmas, España.
4. Brinsko S.P. Insemination doses: how low can we go?. Theriogenology 2006, 66:543-550.
5. Brinsko S.P., Crockett E.C., Squires E.L. Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage. Theriogenology 2000, 54:129-136.
6. Cancel A.M., Lobdell D., Mendola P., Perreault D. Objective evaluation wing assisted reproductive tesperm analysis. Hum. Reprod. 2000, 15:1322-1328.
7. Edmond A.J., Teague S.R., Brinsko S.P., Comerford K.L., Waite J.A., Mancill S.S., Love C.C., Varner D.D. Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa. Anim. Reprod. Sci. 2008, 107:318.
8. Geraghty R.M. Effective removal of equine arteritis virus from stallion semen. Equine Vet. J. 2006, 38:224-229.
9. Johannisson A., Morrell J.M., Thorén J., Jönsson M., Dalin A.-M., Rodriguez-Martinez H. Colloidal centrifugation with Androcoll-ETM prolongs stallion sperm motility, viability and chromatin integrity. Anim. Reprod. Sci. 2009, 116:119-128.
10. Kenney R.M. Manual for clinical fertility evaluation of the stallion, Society for Theriogenology 1983, Hastings, NE.
11. Loomis P.R. Advanced methods for handling and preparation of stallion semen. Vet. Clin. N. Am.: Equine 2006, 22:663-676.
12. Loomis P.R., Graham J.K. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim. Reprod. Sci. 2008, 105:119-128.
13. López-Fernández C., Crespo F., Arroyo F., Fernández J.L., Arana P., Johnston S.D., Gosálvez J. Dynamics of sperm DNA fragmentation in domestic animals II. The stallion. Theriogenology 2007, 68:1240-1250.
14. Love C.C., Kenney R.M. Relationship of sperm chromatin to fertility in the stallion. Proceedings of the 40th Annual Convention AAEP 1994, 21-22.
15. Love C.C., Brinsko S.P., Varner D.D. Use of a silane-coated silica particle solution to enhance the quality of ejaculated semen in stallion. Theriogenology 2002, 58:317-320.
16. Macpherson M.L., Blanchard T.L., Love C.C., Brinsko S.P., Varner D.D. Use of a silane-coated silica particle solution to enhance the quality of ejaculated semen in stallion. Theriogenology 2002, 58:317-320.
17. Morrell J.M., Dalin A.-M., Rodriguez-Martinez H. Prolongation of stallion sperm survival by centrifugation through coated silica colloids: a preliminary study. Anim. Reprod. 2008, 5:121-126.
18. Morrell J.M., Johannisson H., Strutz H., Dalin A.-M., Rodriguez-Martinez H. Colloidal centrifugation of stallion semen: changes in sperm motility, velocity, and chromatin integrity during storage. J. Equine Vet. Sci. 2009, 29:24-32.
19. Morrell J.M., Thorén J., Jönsson M., Dalin A.-M., Rodriguez-Martinez H. Colloidal centrifugation with Androcoll-ETM prolongs stallion sperm motility, viability and chromatin integrity. Anim. Reprod. Sci. 2009, 116:119-128.
20. Ortega-Ferrusola C., González-Fernández L., Muriel A., Macías-García B., Rodríguez-Martínez H., Tapia J.A., Peña F.J. Does the microbial flora in the ejaculate affect the freezeability of stallion sperm?. Reprod. Domest. Anim. 2009, 44:518-522.
21. Samper J.C., Morris C.A. Current methods for stallion semen cryopreservation: a survey. Theriogenology 1998, 49:895-903.
22. Sieme H., Harrison R.A.P., Petrunkina A.M. Cryobiological determinants of frozen semen quality, with special reference to stallion. Anim. Reprod. Sci. 2008, 107:276-292.
23. Varner D.D. Developments in stallion semen evaluation. Theriogenology 2008, 70:448-462.
24. Varner D.D., Love C.C., Brinsko S.P., Blanchard T.L., Bliss S., Carroll S., Eslick M. Semen processing for the subfertile stallion. J. Equine Vet. Sci. 2008, 28:677-685.