Transcriptome analysis of the desert locust central nervous system: Production and annotation of a Schistocerca gregaria EST database

PLoS ONE

Volumn 6, Issue 3, 2011, Pages

  Badisco, Liesbeth ^a   Huybrechts, Jurgen ^a   Simonet, Gert ^a   Verlinden, Heleen ^a   Marchal, Elisabeth ^a   Huybrechts, Roger ^a   Schoofs, Liliane ^a   de Loof, Arnold ^a   Vanden Broeck, Jozef ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

BIOGENIC AMINE; CELL SURFACE RECEPTOR; COMPLEMENTARY DNA; NEUROPEPTIDE; PEPTIDE HORMONE; TRANSCRIPTOME; MESSENGER RNA; PRIMER DNA;

ARTICLE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; DNA LIBRARY; EXPRESSED SEQUENCE TAG; GENE EXPRESSION; GENOME ANALYSIS; INTERMETHOD COMPARISON; LOCUST; NERVE CELL PLASTICITY; NEUROBIOLOGY; NONHUMAN; PHASE TRANSITION; PROTEIN DATABASE; SCHISTOCERCA GREGARIA; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; AMINO ACID SEQUENCE; ANIMAL; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC DATABASE; GENETICS; GRASSHOPPER; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

HEXAPODA; ORTHOPTERA; SCHISTOCERCA GREGARIA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; DATABASES, GENETIC; DNA PRIMERS; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GRASSHOPPERS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIGNAL TRANSDUCTION;

EID: 79952846431     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017274     Document Type: Article

References (226)

1. Stone JV, Mordue W, Batley KE, Morris HR, (1976) Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight. Nature 263: 207-211.
2. Gade G, Goldsworthy GJ, Kegel G, Keller R, (1984) Single step purification of locust adipokinetic hormones I and II by reversed-phase high-performance liquid chromatography, and amino-acid composition of the hormone II. Hoppe-Seyler's Z Physiol Chem 365: 393-398.
3. Schoofs L, Vanden Broeck J, De Loof A, (1993) The myotropic peptides of Locusta migratoria: structures, distribution, functions and receptors. Insect Biochem 23: 859-881.
4. Schoofs L, Veelaert D, Vanden Broeck J, De Loof A, (1997) Peptides in the locusts, Locusta migratoria and Schistocerca gregaria. Peptides 18: 145-156.
5. Clynen E, Schoofs L, (2009) Peptidomic survey of the locust neuroendocrine system. Insect Biochem 39: 491-507.
6. The International Aphid Genomics Consortium (2010) Genome sequence of the pea aphid Acyrthosiphon pisum. PLoS Biol 8: e1000313.
7. Kirkness EF, Haas BJ, Sun W, Braig HR, Perotti MA, et al. (2010) Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle. Proc Natl Acad Sci USA 107: 12168-12173.
8. Wilmore PJ, Brown AK, (1975) Molecular properties of orthopteran DNA. Chromosoma 51: 337-345.
9. Uvarov B, (1966) Grasshoppers and locusts Cambridge Cambridge University Press.
10. Uvarov B, (1977) Grasshoppers and locusts London Centre for overseas pest research.
11. Pener MP, Yerushalmi Y, (1998) The physiology of locust phase polymorphism: an update. J Insect Physiol 44: 365-377.
12. Simpson SJ, Despland E, Hagele BF, Dodgson T, (2001) Gregarious behavior in desert locusts is evoked by touching their back legs. Proc Natl Acad Sci USA 98: 3895-3897.
13. Anstey ML, Rogers SM, Ott SR, Burrows M, Simpson SJ, (2009) Serotonin mediates behavioral gregarization underlying swarm formation in desert locusts. Science 323: 627-630.
14. Rogers SM, Matheson T, Despland E, Dodgson T, Burrows M, et al. (2003) Mechanosensory-induced behavioural gregarization in the desert locust Schistocerca gregaria. J Exp Biol 206: 3991-4002.
15. Rogers SM, Matheson T, Sasaki K, Kendrick K, Simpson SJ, et al. (2004) Substantial changes in central nervous system neurotransmitters and neuromodulators accompany phase change in the locust. J Exp Biol 207: 3603-3617.
16. Lester LR, Grach C, Pener MP, Simpson SJ, (2005) Stimuli inducing gregarious colouration and behaviour in nymphs of Schistocerca gregaria. J Insect Physiol 51: 737-747.
17. Ott SR, Rogers SM, (2010) Gregarious desert locusts have substantially larger brains with altered proportions compared with the solitarious phase. Proc Biol Sci 277: 3087-3096.
18. Withers GS, Fahrbach SE, Robinson GE, (1993) Selective neuroanatomical plasticity and division of labour in the honeybee. Nature 364: 238-240.
19. Durst C, Eichmuller S, Menzel R, (1994) Development and experience lead to increased volume of subcompartments of the honeybee mushroom body. Behav Neural Biol 62: 259-263.
20. Gronenberg W, Heeren S, Holldobler B, (1996) Age-dependent and task-related morphological changes in the brain and the mushroom bodies of the ant Camponotus floridanus. J Exp Biol 199: 2011-2019.
21. Riveros AJ, Gronenberg W, (2010) Brain allometry and neural plasticity in the bumblebee Bombus occidentalis. Brain Behav Evol 75: 138-148.
22. Molina Y, O'Donnell S, (2007) Mushroom body volume is related to social aggression and ovary development in the paperwasp Polistes instabilis. Brain Behav Evol 70: 137-144.
23. Molina Y, O'Donnell S, (2008) Age, sex, and dominance-related mushroom body plasticity in the paperwasp Mischocyttarus mastigophorus. Dev Neurobiol 68: 950-959.
24. Verlinden H, Badisco L, Marchal E, Van Wielendaele P, Vanden Broeck J, (2009) Endocrinology of reproduction and phase transition in locusts. Gen Comp Endocrinol 162: 79-92.
25. Kang L, Chen X, Zhou Y, Liu B, Zheng W, et al. (2004) The analysis of large-scale gene expression correlated to the phase changes of the migratory locust. Proc Natl Acad Sci USA 101: 17611-17615.
26. Ma ZY, Yu J, Kang L, (2006) LocustDB: a relational database for the transcriptome and biology of the migratory locust (Locusta migratoria). BMC Genomics 7: 11.
27. Song H, (2005) Phylogenetic perspectives on the evolution of locust phase polyphenism. J Orthopt Res 14: 235-245.
28. Song H, Wenzel JW, (2008) Phylogeny of bird-grasshopper subfamily Cyrtacanthacridinae (Orthoptera: Acrididae) and the evolution of locust phase polyphenism. Cladistics 24: 515-542.
29. Song H, (2011) Density-dependent phase polyphenism in nonmodel Locusts: a minireview. Psyche (In press).
30. Simpson SJ, Bouai chiA., Roessingh P, (1998) Effects of sensory stimuli on the behavioural phase state of the desert locust, Schistocerca gregaria. J Insect Physiol 44: 883-893.
31. Hagele BF, Simpson SJ, (2000) The influence of mechanical, visual and contact chemical stimulation on the behavioural phase state of solitarious desert locusts (Schistocerca gregaria). J Insect Physiol 46: 1295-1301.
32. Cullen DA, Sword GA, Dodgson T, Simpson SJ, (2010) Behavioural phase change in the Australian plague locust, Chortoicetes terminifera, is triggered by tactile stimulation of the antennae. J Insect Physiol 56: 937-942.
33. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, et al. (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25: 25-29.
34. Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, et al. (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21: 3674-3676.
35. Gotz S, Garcia-Gomez JM, Terol J, Williams TD, Nagaraj SH, et al. (2008) High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res 36: 3420-3435.
36. Claeys I, Poels J, Simonet G, Franssens V, Van Loy T, et al. (2005) Insect neuropeptide and peptide hormone receptors: current knowledge and future directions. Vitam Horm (NY) 73: 217-282.
37. Vanden Broeck J, (1996) G-protein-coupled receptors in insect cells. Int Rev Cytol 164: 189-268.
38. Vanden Broeck J, (2001) Insect G protein-coupled receptors and signal transduction. Arch Insect Biochem Physiol 48: 1-12.
39. Spehr M, Munger SD, (2009) Olfactory receptors: G protein-coupled receptors and beyond. J Neurochem 109: 1570-1583.
40. Morris MB, Dastmalchi S, Church WB, (2009) Rhodopsin: structure, signal transduction and oligomerisation. Int J Biochem Cell Biol 41: 721-724.
41. Hauser F, Cazzamali G, Williamson M, Park Y, Li B, et al. (2008) A genome-wide inventory of neurohormone GPCRs in the red flour beetle Tribolium castaneum. Front Neuroendocrinol 29: 142-165.
42. Hauser F, Cazzamali G, Williamson M, Blenau W, Grimmelikhuijzen CJ, (2006) A review of neurohormone GPCRs present in the fruitfly Drosophila melanogaster and the honey bee Apis mellifera. Prog Neurobiol 80: 1-19.
43. Filmore D, (2004) It's a GPCR world. Modern Drug Discovery 7: 24-28.
44. Towner P, Harris P, Wolstenholme AJ, Hill C, Worm K, et al. (1997) Primary structure of locust opsins: a speculative model which may account for ultraviolet wavelength light detection. Vision Res 37: 495-503.
45. Mertens I, Meeusen T, Huybrechts R, De Loof A, Schoofs L, (2002) Characterization of the short neuropeptide F receptor from Drosophila melanogaster. Biochem Biophys Res Commun 297: 1140-1148.
46. Garczynski SF, Brown MR, Shen P, Murray TF, Crim JW, (2002) Characterization of a functional neuropeptide F receptor from Drosophila melanogaster. Peptides 23: 773-780.
47. Chen ME, Pietrantonio PV, (2006) The short neuropeptide F-like receptor from the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae). Arch Insect Biochem Physiol 61: 195-208.
48. Garczynski SF, Crim JW, Brown MR, (2007) Characterization and expression of the short neuropeptide F receptor in the African malaria mosquito, Anopheles gambiae. Peptides 28: 109-118.
49. Garczynski SF, Crim JW, Brown MR, (2005) Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae. Peptides 26: 99-107.
50. Clynen E, Husson SJ, Schoofs L, (2009) Identification of new members of the (short) neuropeptide F family in locusts and Caenorhabditis elegans. Ann NY Acad Sci 1163: 60-74.
51. Lee KS, You KH, Choo JK, Han YM, Yu K, (2004) Drosophila short neuropeptide F regulates food intake and body size. J Biol Chem 279: 50781-50789.
52. Lee KS, Kwon OY, Lee JH, Kwon K, Min KJ, et al. (2008) Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling. Nat Cell Biol 10: 468-475.
53. Wu Q, Wen T, Lee G, Park JH, Cai HN, et al. (2003) Developmental control of foraging and social behavior by the Drosophila neuropeptide Y-like system. Neuron 39: 147-161.
54. Dierick HA, Greenspan RJ, (2007) Serotonin and neuropeptide F have opposite modulatory effects on fly aggression. Nat Genet 39: 678-682.
55. Yamanaka N, Yamamoto S, Zitnan D, Watanabe K, Kawada T, et al. (2008) Neuropeptide receptor transcriptome reveals unidentified neuroendocrine pathways. PLoS One 3: e3048.
56. Audsley N, Weaver RJ, (2009) Neuropeptides associated with the regulation of feeding in insects. Gen Comp Endocrinol 162: 93-104.
57. Kataoka H, Toschi A, Li JP, Carney RL, Schooley DA, et al. (1989) Identification of an allatotropin from adult Manduca sexta. Science 243: 1481-1483.
58. Pener MP, (1991) Locust phase polymorphism and its endocrine relations. Advances in Insect Physiology London Academic Press Limited.
59. West AP Jr, Llamas LL, Snow PM, Benzer S, Bjorkman PJ, (2001) Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-coupled receptor associated with extended lifespan. Proc Natl Acad Sci USA 98: 3744-3749.
60. Ja WW, West AP Jr, Delker SL, Bjorkman PJ, Benzer S, et al. (2007) Extension of Drosophila melanogaster life span with a GPCR peptide inhibitor. Nat Chem Biol 3: 415-419.
61. Cvejic S, Zhu Z, Felice SJ, Berman Y, Huang XY, (2004) The endogenous ligand Stunted of the GPCR Methuselah extends lifespan in Drosophila. Nat Cell Biol 6: 540-546.
62. Ja WW, Carvalho GB, Madrigal M, Roberts RW, Benzer S, (2009) The Drosophila G protein-coupled receptor, Methuselah, exhibits a promiscuous response to peptides. Protein Sci 18: 2203-2208.
63. Erreger K, Chen PE, Wyllie DJ, Traynelis SF, (2004) Glutamate receptor gating. Crit Rev Neurobiol 16: 187-224.
64. Knopfel T, Uusisaari M, (2008) Modulation of excitation by metabotropic glutamate receptors. Results Probl Cell Differ 44: 163-175.
65. Johnson JW, Ascher P, (1987) Glycine potentiates the NMDA response in cultured mouse brain neurons. Nature 325: 529-531.
66. Skeberdis VA, Lan J, Opitz T, Zheng X, Bennett MV, et al. (2001) mGluR1-mediated potentiation of NMDA receptors involves a rise in intracellular calcium and activation of protein kinase C. Neuropharmacology 40: 856-865.
67. Marshall J, Buckingham SD, Shingai R, Lunt GG, Goosey MW, et al. (1990) Sequence and functional expression of a single alpha subunit of an insect nicotinic acetylcholine receptor. EMBO J 9: 4391-4398.
68. Marshall J, Darlison MG, Lunt GG, Barnard EA, (1988) Cloning of putative nicotinic acetylcholine receptor genes from the locust. Biochem Soc Trans 16: 463-465.
69. Casida JE, (2009) Pest toxicology: the primary mechanisms of pesticide action. Chem Res Toxicol 22: 609-619.
70. Nagatsu T, Levitt M, Udenfriend S, (1964) Tyrosine hydroxylase. The initial step in norepinephrine biosynthesis. J Biol Chem 239: 2910-2917.
71. Mena MA, Casarejos MJ, Solano RM, de Yebenes JG, (2009) Half a century of L-DOPA. Curr Top Med Chem 9: 880-893.
72. Lovenberg W, Weissbach H, Udenfriend S, (1962) Aromatic L-amino acid decarboxylase. J Biol Chem 237: 89-93.
73. Axelrod J, Saavedra JM, (1977) Octopamine. Nature 265: 501-504.
74. Vanden Broeck J, Vulsteke V, Huybrechts R, De Loof A, (1995) Characterization of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila S2 cells. J Neurochem 64: 2387-2395.
75. Verlinden H, Vleugels R, Marchal E, Badisco L, Pfluger HJ, et al. (2010) The role of octopamine in locusts and other arthropods. J Insect Physiol 56: 854-867.
76. Ichiyama A, Nakamura S, Nishizuka Y, Hayaishi O, (1970) Enzymic studies on the biosynthesis of serotonin in mammalian brain. J Biol Chem 245: 1699-1709.
77. Christenson JG, Dairman W, Udenfriend S, (1972) On the identity of DOPA decarboxylase and 5-hydroxytryptophan decarboxylase (immunological titration-aromatic L-amino acid decarboxylase-serotonin-dopamine-norepinephrine). Proc Natl Acad Sci USA 69: 343-347.
78. Wang L, Erlandsen H, Haavik J, Knappskog PM, Stevens RC, (2002) Three-dimensional structure of human tryptophan hydroxylase and its implications for the biosynthesis of the neurotransmitters serotonin and melatonin. Biochemistry 41: 12569-12574.
79. Seidah NG, Chretien M, (1994) Pro-protein convertases of subtilisin/kexin family. Methods Enzymol 244: 175-188.
80. Van de Ven WJ, Voorberg J, Fontijn R, Pannekoek H, van den Ouweland AM, et al. (1990) Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes. Mol Brain Res 14: 265-275.
81. Hatsuzawa K, Murakami K, Nakayama K, (1992) Molecular and enzymatic properties of furin, a Kex2-like endoprotease involved in precursor cleavage at Arg-X-Lys/Arg-Arg sites. J Biochem 111: 296-301.
82. Zhu X, Lindberg I, (1995) 7B2 facilitates the maturation of proPC2 in neuroendocrine cells and is required for the expression of enzymatic activity. J Cell Biol 129: 1641-1650.
83. Benjannet S, Savaria D, Chretien M, Seidah NG, (1995) 7B2 is a specific intracellular binding protein of the prohormone convertase PC2. J Neurochem 64: 2303-2311.
84. Benjannet S, Lusson J, Hamelin J, Savaria D, Chretien M, et al. (1995) Structure-function studies on the biosynthesis and bioactivity of the precursor convertase PC2 and the formation of the PC2/7B2 complex. FEBS Lett 362: 151-155.
85. Lamango NS, Zhu X, Lindberg I, (1996) Purification and enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2. Arch Biochem Biophys 330: 238-250.
86. Seidah NG, Day R, Marcinkiewicz M, Benjannet S, Chretien M, (1991) Mammalian neural and endocrine pro-protein and pro-hormone convertases belonging to the subtilisin family of serine proteinases. Enzyme 45: 271-284.
87. Seidah NG, Day R, Benjannet S, Rondeau N, Boudreault A, et al. (1992) The prohormone and proprotein processing enzymes PC1 and PC2: structure, selective cleavage of mouse POMC and human renin at pairs of basic residues, cellular expression, tissue distribution, and mRNA regulation. NIDA Res Monogr 126: 132-150.
88. Smeekens SP, Avruch AS, LaMendola J, Chan SJ, Steiner DF, (1991) Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans. Proc Natl Acad Sci USA 88: 340-344.
89. Day R, Schafer MK, Watson SJ, Chretien M, Seidah NG, (1992) Distribution and regulation of the prohormone convertases PC1 and PC2 in the rat pituitary. Mol Endocrinol 6: 485-497.
90. Hwang JR, Siekhaus DE, Fuller RS, Taghert PH, Lindberg I, (2000) Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2. Insect cell-specific processing and secretion. J Biol Chem 275: 17886-17893.
91. Macours N, Poels J, Hens K, Francis C, Huybrechts R, (2004) Structure, evolutionary conservation, and functions of angiotensin- and endothelin-converting enzymes. Int Rev Cytol 239: 47-97.
92. Macours N, Hens K, (2004) Zinc-metalloproteases in insects: ACE and ECE. Insect Biochem 34: 501-510.
93. Corvol P, Michaud A, Soubrier F, Williams TA, (1995) Recent advances in knowledge of the structure and function of the angiotensin I converting enzyme. J Hypertens Supplement 3 13: S3-S10.
94. Isaac R, Schoofs L, Williams TA, Veelaert D, Sajid M, et al. (1998) A novel peptide-processing activity of insect peptidyl-dipeptidase A (angiotensin I-converting enzyme): the hydrolysis of lysyl-arginine and arginyl-arginine from the C-terminus of an insect prohormone peptide. Biochem J 330 (Pt 1): 61-65.
95. Schoofs L, Veelaert D, De Loof A, Huybrechts R, Isaac E, (1998) Immunocytochemical distribution of angiotensin I-converting enzyme-like immunoreactivity in the brain and testis of insects. Brain Res 785: 215-227.
96. Isaac RE, Parkin ET, Keen JN, Nassel DR, Siviter RJ, et al. (2002) Inactivation of a tachykinin-related peptide: identification of four neuropeptide-degrading enzymes in neuronal membranes of insects from four different orders. Peptides 23: 725-733.
97. Macours N, Hens K, Francis C, De Loof A, Huybrechts R, (2003) Molecular evidence for the expression of angiotensin converting enzyme in hemocytes of Locusta migratoria: stimulation by bacterial lipopolysaccharide challenge. J Insect Physiol 49: 739-746.
98. Xu D, Emoto N, Giaid A, Slaughter C, Kaw S, et al. (1994) ECE-1: a membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1. Cell 78: 473-485.
99. Macours N, Poels J, Hens K, Luciani N, De Loof A, et al. (2003) An endothelin-converting enzyme homologue in the locust, Locusta migratoria: functional activity, molecular cloning and tissue distribution. Insect Mol Biol 12: 233-240.
100. Fujimura-Kamada K, Nouvet FJ, Michaelis S, (1997) A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor. J Cell Biol 136: 271-285.
101. Tam A, Nouvet FJ, Fujimura-Kamada K, Slunt H, Sisodia SS, et al. (1998) Dual roles for Ste24p in yeast a-factor maturation: NH2-terminal proteolysis and COOH-terminal CAAX processing. J Cell Biol 142: 635-649.
102. Vanden Broeck J, (2001) Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster. Peptides 22: 241-254.
103. Nassel DR, (2002) Neuropeptides in the nervous system of Drosophila and other insects: multiple roles as neuromodulators and neurohormones. Prog Neurobiol 68: 1-84.
104. Schulz-Aellen MF, Roulet E, Fischer-Lougheed J, O'Shea M, (1989) Synthesis of a homodimer neurohormone precursor of locust adipokinetic hormone studied by in vitro translation and cDNA cloning. Neuron 2: 1369-1373.
105. Meredith J, Ring M, Macins A, Marschall J, Cheng NN, et al. (1996) Locust ion transport peptide (ITP): primary structure, cDNA and expression in a baculovirus system. J Exp Biol 199: 1053-1061.
106. Janssen T, Claeys I, Simonet G, De Loof A, Girardie J, et al. (2001) CDNA cloning and transcript distribution of two different neuroparsin precursors in the desert locust, Schistocerca gregaria. Insect Mol Biol 10: 183-189.
107. Vanden Broeck J, Veelaert D, Bendena WG, Tobe SS, De Loof A, (1996) Molecular cloning of the precursor cDNA for schistostatins, locust allatostatin-like peptides with myoinhibiting properties. Mol Cell Endocrinol 122: 191-198.
108. Goldsworthy GJ, Lee MJ, Luswata R, Drake AF, Hyde D, (1997) Structures, assays and receptors for locust adipokinetic hormones. Comp Biochem Physiol B, Biochem Mol Biol 117: 483-496.
109. Van der Horst DJ, Van Marrewijk WJ, Diederen JH, (2001) Adipokinetic hormones of insect: release, signal transduction, and responses. Int Rev Cytol 211: 179-240.
110. Gade G, (2009) Peptides of the adipokinetic hormone/red pigment-concentrating hormone family: a new take on biodiversity. Ann NY Acad Sci 1163: 125-136.
111. Siegert KJ, (1999) Locust corpora cardiaca contain an inactive adipokinetic hormone. FEBS Lett 447: 237-240.
112. Woodhead AP, Stay B, Seidel SL, Khan MA, Tobe SS, (1989) Primary structure of four allatostatins: neuropeptide inhibitors of juvenile hormone synthesis. Proc Natl Acad Sci USA 86: 5997-6001.
113. Pratt GE, Farnsworth DE, Siegel NR, Fok KF, Feyereisen R, (1989) Identification of an allatostatin from adult Diploptera punctata. Biochem Biophys Res Commun 163: 1243-1247.
114. Bendena WG, Donly BC, Tobe SS, (1999) Allatostatins: a growing family of neuropeptides with structural and functional diversity. Ann NY Acad Sci 897: 311-329.
115. Gade G, Hoffmann KH, (2005) Neuropeptides regulating development and reproduction in insects. Physiol Entomol 30: 103-121.
116. Veelaert D, Passier P, Devreese B, Vanden Broeck J, Van Beeumen J, et al. (1997) Isolation and characterization of an adipokinetic hormone release-inducing factor in locusts: the crustacean cardioactive peptide. Endocrinology 138: 138-142.
117. Veenstra JA, (2009) Allatostatin C and its paralog allatostatin double C: the arthropod somatostatins. Insect Biochem 39: 161-170.
118. Weaver RJ, Audsley N, (2009) Neuropeptide regulators of juvenile hormone synthesis: structures, functions, distribution, and unanswered questions. Ann NY Acad Sci 1163: 316-329.
119. Paemen L, Tips A, Schoofs L, Proost P, Van Damme J, et al. (1991) Lom-AG-myotropin: a novel myotropic peptide from the male accessory glands of Locusta migratoria. Peptides 12: 7-10.
120. Schoofs L, Holman GM, Hayes TK, Kochansky JP, Nachman RJ, et al. (1990) Locustatachykinin III and IV: two additional insect neuropeptides with homology to peptides of the vertebrate tachykinin family. Regul Pept 31: 199-212.
121. Schoofs L, Holman GM, Hayes TK, Nachman RJ, De Loof A, (1990) Locustatachykinin I and II, two novel insect neuropeptides with homology to peptides of the vertebrate tachykinin family. FEBS Lett 261: 397-401.
122. Clynen E, Huybrechts J, Verleyen P, De Loof A, Schoofs L, (2006) Annotation of novel neuropeptide precursors in the migratory locust based on transcript screening of a public EST database and mass spectrometry. BMC Genomics 7: 201.
123. Minth CD, Bloom SR, Polak JM, Dixon JE, (1984) Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine. Proc Natl Acad Sci USA 81: 4577-4581.
124. Curry WJ, Shaw C, Johnston CF, Thim L, Buchanan KD, (1992) Neuropeptide F: primary structure from the tubellarian, Artioposthia triangulata. Comp Biochem Physiol C, Comp Pharmacol Toxicol 101: 269-274.
125. Maule AG, Shaw C, Halton DW, Brennan GP, Johnston CF, et al. (1992) Neuropeptide F (Moniezia expansa): localization and characterization using specific antisera. Parasitology 105 (Pt 3): 505-512.
126. Leung PS, Shaw C, Maule AG, Thim L, Johnston CF, et al. (1992) The primary structure of neuropeptide F (NPF) from the garden snail, Helix aspersa. Regul Pept 41: 71-81.
127. Rajpara SM, Garcia PD, Roberts R, Eliassen JC, Owens DF, et al. (1992) Identification and molecular cloning of a neuropeptide Y homolog that produces prolonged inhibition in Aplysia neurons. Neuron 9: 505-513.
128. Brown MR, Crim JW, Arata RC, Cai HN, Chun C, et al. (1999) Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family. Peptides 20: 1035-1042.
129. Audsley N, McIntosh C, Phillips JE, (1992) Isolation of a neuropeptide from locust corpus cardiacum which influences ileal transport. J Exp Biol 173: 261-274.
130. Phillips JE, Wiens C, Audsley N, Jeffs L, Bilgen T, et al. (1996) Nature and control of chloride transport in insect absorptive epithelia. J Exp Zool 275: 292-299.
131. Macins A, Meredith J, Zhao Y, Brock HW, Phillips JE, (1999) Occurrence of ion transport peptide (ITP) and ion transport-like peptide (ITP-L) in orthopteroids. Arch Insect Biochem Physiol 40: 107-118.
132. Huybrechts J, Clynen E, De Loof A, Schoofs L, (2003) Osmotic lysis of corpora cardiaca using distilled water reveals the presence of partially processed peptides and peptide fragments. Physiol Entomol 28: 46-53.
133. Boureme D, Fournier B, Matz G, Girardie J, (1989) Immunological and functional cross-reactivities between locust neuroparsins and proteins from cockroach corpora cardiaca. J Insect Physiol 35: 265-271.
134. Girardie J, Girardie A, Huet JC, Pernollet JC, (1989) Amino acid sequence of locust neuroparsins. FEBS Lett 245: 4-8.
135. Hietter H, Vandorsselaer A, Luu B, (1991) Characterization of 3 structurally related 8-9 Kda monomeric peptides present in the corpora cardiaca of Locusta- A revised structure for the neuroparsins. Insect Biochem 21: 259-264.
136. Lagueux M, Kromer E, Girardie J, (1992) Cloning of a Locusta cDNA encoding neuroparsin-A. Insect Biochem 22: 511-516.
137. Fournier B, Herault JP, Proux J, (1987) Antidiuretic factor from the nervous corpora cardiaca of the migratory locust- Improvement of an existing in vitro bioassay. Gen Comp Endocrinol 68: 49-56.
138. Vanhems E, Delbos M, Girardie J, (1990) Insulin and neuroparsin promote neurite outgrowth in cultured locust CNS. Eur J Neurosci 2: 776-782.
139. Moreau R, Gourdoux L, Girardie J, (1988) Neuroparsin- a new energetic neurohormone in the African locust. Arch Insect Biochem Physiol 8: 135-145.
140. Girardie J, Huet JC, Atay-Kadiri Z, Ettaouil S, Delbecque JP, et al. (1998) Isolation, sequence determination, physical and physiological characterization of the neuroparsins and ovary maturing parsins of Schistocerca gregaria. Insect Biochem 28: 641-650.
141. Claeys I, Simonet G, Van Loy T, De Loof A, Vanden Broeck J, (2003) cDNA cloning and transcript distribution of two novel members of the neuroparsin family in the desert locust, Schistocerca gregaria. Insect Mol Biol 12: 473-481.
142. Badisco L, Claeys I, Van Loy T, Van Hiel MB, Franssens V, et al. (2007) Neuroparsins, a family of conserved arthropod neuropeptides. Gen Comp Endocrinol 153: 64-71.
143. Badisco L, Claeys I, Van Hiel MB, Clynen E, Huybrechts J, et al. (2008) Purification and characterization of an insulin-related peptide in the desert locust, Schistocerca gregaria: immunolocalization, cDNA cloning, transcript profiling and interaction with neuroparsin. J Mol Endocrinol 40: 137-150.
144. Claeys I, Simonet G, Breugelmans B, Van Soest S, Franssens V, et al. (2005) Quantitative real-time RT-PCR analysis in desert locusts reveals phase dependent differences in neuroparsin transcript levels. Insect Mol Biol 14: 415-422.
145. Claeys I, Breugelmans B, Simonet G, Van Soest S, Sas F, et al. (2006) Neuroparsin transcripts as molecular markers in the process of desert locust (Schistocerca gregaria) phase transition. Biochem Biophys Res Commun 341: 599-606.
146. Roller L, Yamanaka N, Watanabe K, Daubnerova I, Zitnan D, et al. (2008) The unique evolution of neuropeptide genes in the silkworm Bombyx mori. Insect Biochem 38: 1147-1157.
147. Boerjan B, Cardoen D, Bogaerts A, Landuyt B, Schoofs L, et al. (2010) Mass spectrometric profiling of (neuro)-peptides in the worker honeybee, Apis mellifera. Neuropharmacology 58: 248-258.
148. Stangier J, Hilbich C, Beyreuther K, Keller R, (1987) Unusual cardioactive peptide (CCAP) from pericardial organs of the shore crab Carcinus maenas. Proc Natl Acad Sci USA 84: 575-579.
149. Stangier J, Hilbich C, Keller R, (1989) Occurrence of crustacean cardioactive peptide (CCAP) in the nervous system of an insect, Locusta migratoria. J Comp Physiol B, Biochem Syst Environ Physiol 159: 5-11.
150. Donini A, Agricola H, Lange AB, (2001) Crustacean cardioactive peptide is a modulator of oviduct contractions in Locusta migratoria. J Insect Physiol 47: 277-285.
151. Tublitz NJ, Truman JW, (1985) Intracellular stimulation of an identified neuron evokes cardioacceleratory peptide release. Science 228: 1013-1015.
152. Tublitz NJ, Truman JW, (1985) Identification of neurones containing cardioacceleratory peptides (CAPs) in the ventral nerve cord of the tobacco hawkmoth, Manduca sexta. J Exp Biol 116: 395-410.
153. Tublitz NJ, Truman JW, (1985) Insect cardioactive peptides. I. Distribution and molecular characteristics of two cardioacceleratory peptides in the tobacco hawkmoth, Manduca sexta. J Exp Biol 114: 365-379.
154. Tublitz NJ, Truman JW, (1985) Insect cardioactive peptides. II. Neurohormonal control of heart activity by two cardioacceleratory peptides in the tobacco hawkmoth, Manduca sexta. J Exp Biol 114: 381-395.
155. Huesmann GR, Cheung CC, Loi PK, Lee TD, Swiderek KM, et al. (1995) Amino acid sequence of CAP2b, an insect cardioacceleratory peptide from the tobacco hawkmoth Manduca sexta. FEBS Lett 371: 311-314.
156. Wegener C, Herbert Z, Eckert M, Predel R, (2002) The periviscerokinin (PVK) peptide family in insects: evidence for the inclusion of CAP(2b) as a PVK family member. Peptides 23: 605-611.
157. Wegener C, Predel R, Eckert M, (1999) Quantification of periviscerokinin-1 in the nervous system of the American cockroach, Periplaneta americana. An insect neuropeptide with unusual distribution. Arch Insect Biochem Physiol 40: 203-211.
158. Clynen E, Huybrechts J, De Loof A, Schoofs L, (2003) Mass spectrometric analysis of the perisympathetic organs in locusts: identification of novel periviscerokinins. Biochem Biophys Res Commun 300: 422-428.
159. Predel R, Wegener C, (2006) Biology of the CAPA peptides in insects. Cell Mol Life Sci 63: 2477-2490.
160. Lehmberg E, Ota RB, Furuya K, King DS, Applebaum SW, et al. (1991) Identification of a diuretic hormone of Locusta migratoria. Biochem Biophys Res Commun 179: 1036-1041.
161. Kay I, Wheeler CH, Coast GM, Totty NF, Cusinato O, et al. (1991) Characterization of a diuretic peptide from Locusta migratoria. Bio Chem Hoppe-Seyler 372: 929-934.
162. Kataoka H, Troetschler RG, Li JP, Kramer SJ, Carney RL, et al. (1989) Isolation and identification of a diuretic hormone from the tobacco hornworm, Manduca sexta. Proc Natl Acad Sci USA 86: 2976-2980.
163. Huising MO, Flik G, (2005) The remarkable conservation of corticotropin-releasing hormone (CRH)-binding protein in the honeybee (Apis mellifera) dates the CRH system to a common ancestor of insects and vertebrates. Endocrinology 146: 2165-2170.
164. Girardie J, Richard O, Huet JC, Nespoulous C, Van Dorsselaer A, et al. (1991) Physical characterization and sequence identification of the ovary maturating parsin. A new neurohormone purified from the nervous corpora cardiaca of the African locust (Locusta migratoria migratorioides). Eur J Biochem 202: 1121-1126.
165. Fraenkel G, Hsiao C, Seligman M, (1966) Properties of bursicon: an insect protein hormone that controls cuticular tanning. Science 151: 91-93.
166. Dewey EM, McNabb SL, Ewer J, Kuo GR, Takanishi CL, et al. (2004) Identification of the gene encoding bursicon, an insect neuropeptide responsible for cuticle sclerotization and wing spreading. Curr Biol 14: 1208-1213.
167. Honegger HW, Dewey EM, Ewer J, (2008) Bursicon, the tanning hormone of insects: recent advances following the discovery of its molecular identity. J Comp Physiol A, Neuroethol Sens Neural Behav Physiol 194: 989-1005.
168. Luan H, Lemon WC, Peabody NC, Pohl JB, Zelensky PK, et al. (2006) Functional dissection of a neuronal network required for cuticle tanning and wing expansion in Drosophila. J Neurosci 26: 573-584.
169. Mendive FM, Van Loy T, Claeysen S, Poels J, Williamson M, et al. (2005) Drosophila molting neurohormone bursicon is a heterodimer and the natural agonist of the orphan receptor DLGR2. FEBS Lett 579: 2171-2176.
170. Luo CW, Dewey EM, Sudo S, Ewer J, Hsu SY, et al. (2005) Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2. Proc Natl Acad Sci USA 102: 2820-2825.
171. Van Loy T, Van Hiel MB, Vandersmissen HP, Poels J, Mendive F, et al. (2007) Evolutionary conservation of bursicon in the animal kingdom. Gen Comp Endocrinol 153: 59-63.
172. Corless CL, Matzuk MM, Ramabhadran TV, Krichevsky A, Boime I, (1987) Gonadotropin beta subunits determine the rate of assembly and the oligosaccharide processing of hormone dimer in transfected cells. J Cell Biol 104: 1173-1181.
173. Boime I, Ben-Menahem D, (1999) Glycoprotein hormone structure-function and analog design. Recent Prog Horm Res 54: 271-288.
174. Querat B, Sellouk A, Salmon C, (2000) Phylogenetic analysis of the vertebrate glycoprotein hormone family including new sequences of sturgeon (Acipenser baeri) beta subunits of the two gonadotropins and the thyroid-stimulating hormone. Biol Reprod 63: 222-228.
175. Kwok HF, So WK, Wang Y, Ge W, (2005) Zebrafish gonadotropins and their receptors: I. Cloning and characterization of zebrafish follicle-stimulating hormone and luteinizing hormone receptors-evidence for their distinct functions in follicle development. Biol Reprod 72: 1370-1381.
176. So WK, Kwok HF, Ge W, (2005) Zebrafish gonadotropins and their receptors: II. Cloning and characterization of zebrafish follicle-stimulating hormone and luteinizing hormone subunits-their spatial-temporal expression patterns and receptor specificity. Biol Reprod 72: 1382-1396.
177. Hsu SY, Nakabayashi K, Bhalla A, (2002) Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5. Mol Endocrinol 16: 1538-1551.
178. Macdonald LE, Wortley KE, Gowen LC, Anderson KD, Murray JD, et al. (2005) Resistance to diet-induced obesity in mice globally overexpressing OGH/GPB5. Proc Natl Acad Sci USA 102: 2496-2501.
179. Sudo S, Kuwabara Y, Park JI, Hsu SY, Hsueh AJ, (2005) Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich repeat-containing G protein-coupled receptor-1 (DLGR1) and stimulation of human thyrotropin receptors by chimeric fly GPA2 and human GPB5. Endocrinology 146: 3596-3604.
180. Baggerman G, Cerstiaens A, De Loof A, Schoofs L, (2002) Peptidomics of the larval Drosophila melanogaster central nervous system. J Biol Chem 277: 40368-40374.
181. Hummon AB, Richmond TA, Verleyen P, Baggerman G, Huybrechts J, et al. (2006) From the genome to the proteome: uncovering peptides in the Apis brain. Science 314: 647-649.
182. Li B, Predel R, Neupert S, Hauser F, Tanaka Y, et al. (2008) Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum. Genome Res 18: 113-122.
183. Riehle MA, Garczynski SF, Crim JW, Hill CA, Brown MR, (2002) Neuropeptides and peptide hormones in Anopheles gambiae. Science 298: 172-175.
184. Verleyen P, Chen X, Baron S, Preumont A, Hua YJ, et al. (2009) Cloning of neuropeptide-like precursor 1 in the gray flesh fly and peptide identification and expression. Peptides 30: 522-530.
185. Ma M, Gard AL, Xiang F, Wang J, Davoodian N, et al. (2009) Combining in silico transcriptome mining and biological mass spectrometry for neuropeptide discovery in the Pacific white shrimp Litopenaeus vannamei. Peptides 31: 27-43.
186. Rahman MM, Breuer M, Begum M, Baggerman G, Huybrechts J, et al. (2008) Localization of the phase-related 6-kDa peptide (PRP) in different tissues of the desert locust Schistocerca gregaria-immunocytochemical and mass spectrometric approach. J Insect Physiol 54: 543-554.
187. Rahman MM, Vandingenen A, Begum M, Breuer M, De Loof A, et al. (2003) Search for phase specific genes in the brain of desert locust, Schistocerca gregaria (Orthoptera: Acrididae) by differential display polymerase chain reaction. Comp Biochem Physiol A, Comp Physiol 135: 221-228.
188. Rahman MM, Vanden Bosch L, Baggerman G, Clynen E, Hens K, et al. (2002) Search for peptidic molecular markers in hemolymph of crowd-(gregarious) and isolated-reared (solitary) desert locusts, Schistocerca gregaria. Peptides 23: 1907-1914.
189. Sage H, Vernon RB, Funk SE, Everitt EA, Angello J, (1989) SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J Cell Biol 109: 341-356.
190. Sage H, Vernon RB, Decker J, Funk S, Iruela-Arispe ML, (1989) Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice. J Histochem Cytochem 37: 819-829.
191. Sage H, Decker J, Funk S, Chow M, (1989) SPARC: a Ca2+-binding extracellular protein associated with endothelial cell injury and proliferation. J Mol Cell Cardiol 21 (Suppl 1): 13-22.
192. Golembieski WA, Ge S, Nelson K, Mikkelsen T, Rempel SA, (1999) Increased SPARC expression promotes U87 glioblastoma invasion in vitro. Int J Dev Neurosci 17: 463-472.
193. Brekken RA, Sage EH, (2001) SPARC, a matricellular protein: at the crossroads of cell-matrix communication. Matrix Biol 19: 816-827.
194. Murphy-Ullrich JE, Lightner VA, Aukhil I, Yan YZ, Erickson HP, et al. (1991) Focal adhesion integrity is downregulated by the alternatively spliced domain of human tenascin. J Cell Biol 115: 1127-1136.
195. Rosenblatt S, Bassuk JA, Alpers CE, Sage EH, Timpl R, et al. (1997) Differential modulation of cell adhesion by interaction between adhesive and counter-adhesive proteins: characterization of the binding of vitronectin to osteonectin (BM40, SPARC). Biochem J 324 (Pt 1): 311-319.
196. Funk SE, Sage EH, (1991) The Ca2(+)-binding glycoprotein SPARC modulates cell cycle progression in bovine aortic endothelial cells. Proc Natl Acad Sci USA 88: 2648-2652.
197. Tremble PM, Lane TF, Sage EH, Werb Z, (1993) SPARC, a secreted protein associated with morphogenesis and tissue remodeling, induces expression of metalloproteinases in fibroblasts through a novel extracellular matrix-dependent pathway. J Cell Biol 121: 1433-1444.
198. Barker TH, Baneyx G, Cardo-Vila M, Workman GA, Weaver M, et al. (2005) SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity. J Biol Chem 280: 36483-36493.
199. Kupprion C, Motamed K, Sage EH, (1998) SPARC (BM-40, osteonectin) inhibits the mitogenic effect of vascular endothelial growth factor on microvascular endothelial cells. J Biol Chem 273: 29635-29640.
200. Hasselaar P, Sage EH, (1992) SPARC antagonizes the effect of basic fibroblast growth factor on the migration of bovine aortic endothelial cells. J Cell Biochem 49: 272-283.
201. Wu RX, Laser M, Han H, Varadarajulu J, Schuh K, et al. (2006) Fibroblast migration after myocardial infarction is regulated by transient SPARC expression. J Mol Med 84: 241-252.
202. Raines EW, Lane TF, Iruela-Arispe ML, Ross R, Sage EH, (1992) The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and-BB and inhibits the binding of PDGF to its receptors. Proc Natl Acad Sci USA 89: 1281-1285.
203. Gongidi V, Ring C, Moody M, Brekken R, Sage EH, et al. (2004) SPARC-like 1 regulates the terminal phase of radial glia-guided migration in the cerebral cortex. Neuron 41: 57-69.
204. Kidd T, Bland KS, Goodman CS, (1999) Slit is the midline repellent for the robo receptor in Drosophila. Cell 96: 785-794.
205. Dimitrova S, Reissaus A, Tavosanis G, (2008) Slit and Robo regulate dendrite branching and elongation of space-filling neurons in Drosophila. Dev Biol 324: 18-30.
206. Brierley DJ, Blanc E, Reddy OV, Vijayraghavan K, Williams DW, (2009) Dendritic targeting in the leg neuropil of Drosophila: the role of midline signalling molecules in generating a myotopic map. PLoS Biol 7: e1000199.
207. Boyan G, Therianos S, Williams JL, Reichert H, (1995) Axogenesis in the embryonic brain of the grasshopper Schistocerca gregaria: an identified cell analysis of early brain development. Development 121: 75-86.
208. Geva N, Guershon M, Orlova M, Ayali A, (2009) Memoirs of a locust: density-dependent behavioral change as a model for learning and memory. Neurobiol Learn Mem 93: 175-182.
209. Dukas R, (2008) Evolutionary biology of insect learning. Annu Rev Entomol 53: 145-160.
210. Kloppenburg P, Mercer AR, (2008) Serotonin modulation of moth central olfactory neurons. Annu Rev Entomol 53: 179-190.
211. Mercer AR, Kirchhof BS, Hildebrand JG, (1996) Enhancement by serotonin of the growth in vitro of antennal lobe neurons of the sphinx moth Manduca sexta. J Neurobiol 29: 49-64.
212. Mayford M, Barzilai A, Keller F, Schacher S, Kandel ER, (1992) Modulation of an NCAM-related adhesion molecule with long-term synaptic plasticity in Aplysia. Science 256: 638-644.
213. Ortiz de Montellano PR, De Voss JJ, (2005) Substrate oxidation by Cytochrome P450 enzymes. In: Ortiz de Montellano PR, editors. Cytochrome P450: Structure, Mechanism, and Biochemistry New York Kluwer Academic/Plenum Publishers pp. 183-246.
214. Wassarman DA, Steitz JA, (1991) RNA splicing. Alive with DEAD proteins. Nature 349: 463-464.
215. Schmid SR, Linder P, (1992) D-E-A-D protein family of putative RNA helicases. Mol Microbiol 6: 283-291.
216. Berthier K, Chapuis MP, Simpson SJ, Ferenz HJ, Habib Kane CM, et al. (2010) Laboratory populations as a resource for understanding the relationship between genotypes and phenotypes: A global case study in locusts. Advances in Insect Physiology 39: 1-37.
217. Hoste B, Luyten L, Claeys I, Clynen E, Rahman MM, et al. (2002) An improved breeding method for solitarious locusts. Entomol Exp Appl 104: 281-288.
218. Bonaldo MF, Lennon G, Soares MB, (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6: 791-806.
219. Ewing B, Green P, (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8: 186-194.
220. Ewing B, Hillier L, Wendl MC, Green P, (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 8: 175-185.
221. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
222. Kumar CG, LeDuc R, Gong G, Roinishivili L, Lewin HA, et al. (2004) ESTIMA, a tool for EST management in a multi-project environment. BMC Bioinformatics 5: 176.
223. Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, et al. (2005) InterProScan: protein domains identifier. Nucleic Acids Res 33: W116-W120.
224. Hunter S, Apweiler R, Attwood TK, Bairoch A, Bateman A, et al. (2009) InterPro: the integrative protein signature database. Nucleic Acids Res 37: D211-D215.
225. Van Hiel MB, Van Wielendaele P, Temmerman L, Van Soest S, Vuerinckx K, et al. (2009) Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. BMC Mol Biol 10: 56.
226. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.