Effects on brown bear (Ursus arctos) spermatozoa freezability of different extender and dilution ratios used for pre-freezing centrifugation

European Journal of Wildlife Research

Volumn 57, Issue 2, 2011, Pages 259-266

  Nicolas, Maria ^a   Alvarez, Mercedes ^a   Gomes Alves, Susana ^a   Mata Campuzano, Maria ^a   Borragán, Santiago ^b   Martinez Pastor, Felipe ^a   de Paz, Paulino ^a   Anel, Luis ^a  

^a UNIVERSITY OF LEÓN   (Spain)

^b Cabarceno Park   (Spain)

Author keywords

Brown bear; Centrifugation; Dilution; Extender; Spermatozoa

Indexed keywords

URSUS ARCTOS;

EID: 79952814792     PISSN: 16124642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10344-010-0420-y     Document Type: Article

References (37)

1. Aitken RJ, Clarkson JS (1988) Significance of reactive oxygen species and antioxidants in defining of the efficiency of sperm preparation technique. J Androl 9: 367-376.
2. Althouse GC, Seager SWJ, Varner DD, Webb GW (1989) Diagnostic aids for the detection of urine in the equine ejaculate. Theriogenology 31: 1141-1148.
3. Alvarez JG, Lasso JL, Blasco L, Nuñez RC, Heyner S, Caballero PP, Storey BT (1993) Centrifugation of human spermatozoa induces sublethal damage; separation of human spermatozoa from seminal plasma by a dextran swim-up procedure without centrifugation extends their motile lifetime. Hum Reprod 8(7): 1087-1092.
4. Anel L, de Paz P, Alvarez M, Chamorro CA, Boixo JC, Manso A, Gonzalez M, Kaabi M, Anel E (2003) Field and in vitro assay of three methods for freezing ram semen. Theriogenology 60: 1293-1308.
5. Anel L, Alvarez M, Martínez-Pastor F, Gomes S, Nicolás M, Mata M, Martínez AF, Borragán S, Anel E, de Paz P (2008) Sperm cryopreservation in brown bear (Ursus arctos): preliminary result. Reprod Domest Anim 43: 9-17.
6. Anel L, Gomes-Alves S, Alvarez M, Borragan S, Anel E, Nicolas M, Martinez-Pastor F, de Paz P (2010) Effect of basic factors of extender composition on post-thawing quality of brown bear electroejaculated spermatozoa. Theriogenology. doi: 10. 1016/j. theriogenology. 2010. 03. 004.
7. Brinsko SP, Crockett EC, Squire EL (2000) Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage. Theriogenology 54: 129-136.
8. Cardullo RA, Cone RA (1986) Mechanical immobilization of rat sperm does not exchange their oxygen consumption rate. Biol Reprod 34: 820-830.
9. Carvajal G, Cuello C, Ruiz M, Vazquez JM, Martinez EA, Roca F (2004) Effects of centrifugation before freezing on boar sperm cryosurvival. J Androl 25: 389-396.
10. Carver DA, Ball BA (2002) Lipase activity in stallion seminal plasma and the effect of lipase on stallion spermatozoa during storage at 5 degrees C. Theriogenology 58(8): 1587-1595.
11. Chen LM, Hou R, Zhang ZH, Wang JS, An XR, Chen YF, Zheng HP, Xia GL, Zhang MJ (2007) Electroejaculation and semen characteristics of Asiatic Black bears (Ursus thibetanus). Anim Reprod Sci 101: 358-364.
12. Crockett EC, Graham JK, Bruemmer JE, Squires EL (2001) Effect of cooling on equine spermatozoa before freezing on post-thaw motility: preliminary results. Theriogenology 55: 793-803.
13. García-Macías V, Martínez-Pastor F, Álvarez M, Paz P, Borragán S, Anel E, Mata M, Anel L (2007) Use of a triple-stain (SYBR-14/PI/MC540) for viability and capacitation assessment in thawed semen from brown bear (Ursus arctos). Reprod Fertil Dev 19(1): 239.
14. Ishikawa A, Matsui M, Sakamoto H, Katagiri S, Takahashi Y (2001) Cryopreservation of the semen collected by electroejaculation from the Hokkaido brown bear (Ursus arctos yesoensis). J Vet Sci 64(4): 373-376.
15. Katkov II, Mazur P (1998) Influence of centrifugation regimes on motility, yield and cell associations of mouse spermatozoa. J Androl 19: 232-241.
16. Katkov II, Ostashko FI (1996) Correlation between electromechanical stability of cytoplasm membrane and cryoresistivity of bovine spermatozoa. Cryobiology 33: 680-687.
17. Kim SC, Kim HW (1998) Effects of nitrogenous components of urine on sperm motility: an in vitro study. Int J Androl 21(1): 29-33.
18. Kojima E, Tsuruga H, Komatsu T, Murase T, Tsubota TM, Kita I (2001) Characterization of semen collected from beagles and captive Japanese black bears (Ursus thibetanus japonicus). Theriogenology 55: 717-731.
19. Makler A, David R, Blumenfeld Z, Better OS (1981) Factors affecting sperm motility. VII. Sperm viability as affected by change of pH and osmolarity of semen and urine specimens. Fertil Steril 36(4): 507-511.
20. Martinez-Pastor F, Anel L, Guerra C, Alvarez M, Soler AJ, Garde JJ, Chamorro C, de PP (2006) Seminal plasma improves cryopreservation of Iberian red deer epididymal sperm. Theriogenology 66: 1847-1856.
21. Matas C, Decuadro G, Martinez-Miro S, Gadea J (2007) Evaluation of a cushioned method for centrifugation and processing for freezing boar semen. Theriogenology 67: 1087-1091.
22. McMahon CG, Abdo C, Incrocci L, Perelman M, Rowland D, Waldinger M, Xin ZC (2004) Disorders of orgasm and ejaculation in men. The Journal of Sexual Medicine 1: 58-65.
23. Mortimer D, Goel N, Shu MA (1988) Evaluation of the cell soft automated semen analysis system in a routine laboratory setting. Fertil Steril 50: 960-968.
24. Ng SC, Bongso TA, Sathananthan H, Tok VCN, Ratnam SS (1990) Microcentrifugation of human spermatozoa: its effect on fertilization of hamster oocytes after micro-insemination spermatozoal transfer. Hum Reprod 5: 209-211.
25. Okano T, Murase T, Tsubota T (2004) Electroejaculation and semen cryopreservation of free-ranging Japanese black bears (Ursus thibetanus japonicus). J Vet Med Sci 66(11): 1371-1376.
26. Ollero M, Cebrian-Pérez JA, Muiño-Blando T (1997) Improvement of cryopreserved ram sperm heterogeneity and viability by addition of seminal plasma. J Androl 18(6): 732-739.
27. Pellicer-Rubio MT, Magallon T, Combarnous Y (1997) Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity. Biol Reprod 57: 1023-1031.
28. Pickett BW, Sullivan JJ, Byers WW, Pace MM, Remmenga EE (1975) Effect of cenrifugation and seminal plasma on motility and fertility of stallion and bull spermatozoa. Fertil Steril 26: 167-174.
29. R Development Core Team (2009) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, ISBN 3-900051-07-0, http://www. R-project. org.
30. Rijsselaere T, van Soom A, Maes D, de Kruif A (2002) Effect of centrifugation on in vitro survival of fresh diluted canine spermatozoa. Theriogenology 57: 1669-1681.
31. Ritar AJ, Salamon S (1982) Effects of seminal plasma and of its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat. Aust J Bil Sci 35(3): 305-312.
32. Rota A, Milani C, Romagnoli S (2006) Effect of post-thaw dilution whit autologous prostatic fluid on dog semen motility and sperm acrosome status. Theriogenology 67: 520-525.
33. Shäfer-Somi S, Kluger S, Knapp E, Klein D, Aurich C (2006) Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa. Theriogenology 66(2): 173-182.
34. Sieme H, Katila T, Klug E (2004) Effect of semen collection practices on sperm characteristics before and after storage and on fertility of stallions. Theriogenology 61: 769-784.
35. Varisli O, Uguz C, Agca C, Agca Y (2009) Effect of various physical stress factors on rat sperm motility, acrosome and plasma membrane integrity. J Androl 30: 75-86.
36. Waite JA, Love CC, Brinsko SP, Teague SR, Salazar JL Jr, Mancill SS, Varner DD (2008) Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Theriogenology 70: 704-714.
37. Way AL, Lester CG, Killian GJ (2000) Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa. J Androl 21: 213-219.