Erythrocyte membrane protein analysis by sodium dodecyl sulphate-capillary gel electrophoresis in the diagnosis of hereditary spherocytosis

Clinical Chemistry and Laboratory Medicine

Volumn 49, Issue 3, 2011, Pages 485-492

  Debaugnies, France ^a   Cotton, Frédéric ^a   Boutique, Charles ^a   Gulbis, Béatrice ^a  

^a ERASME HOSPITAL   (Belgium)

Author keywords

capillary gel electrophoresis; erythrocyte; membrane proteins

Indexed keywords

ACTIN; ANKYRIN; ERYTHROCYTE BAND 3 PROTEIN; ERYTHROCYTE BAND 4.1 PROTEIN; ERYTHROCYTE BAND 4.2 PROTEIN; FODRIN;

ANIMAL CELL; ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; HEREDITARY ELLIPTOCYTOSIS; HEREDITARY SPHEROCYTOSIS; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTE MEMBRANE; ERYTHROCYTES; HUMANS; MEMBRANE PROTEINS; SPHEROCYTOSIS, HEREDITARY;

EID: 79952777362     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2011.066     Document Type: Article

References (22)

1. Gulbis B, Eleftheriou A, Angastiniotis M, Ball S, Surralès J, Castella M, et al. Epidemiology of rare anaemias in Europe. Adv Exp Med Biol 2010;686:375-96.
2. Tse WT, Lux SE. Red blood cell membrane disorders. Br J Haematol 1999;104:2-13. (Pubitemid 29037011)
3. Bolton-Maggs PH, Stevens RF, Dodd NJ, Lamont G, Tittensor P, King MJ. Guidelines for the diagnosis and management of hereditary spherocytosis. Br J Haematol 2004;126:455-74. (Pubitemid 39093088)
4. Dodge JT, Mitchell C, Hanahan DJ. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys 1963;100:119-30.
5. Lin C, Cotton F, Boutique C, Dhermy D, Vertongen F, Gulbis B. Capillary gel electrophoresis: Separation of major erythrocyte membrane proteins. J Chromatogr B Biomed Sci Appl 2000;742:411-9. (Pubitemid 30331059)
6. Tyler JM, Hargreaves WR, Branton D. Purification of two spectrin- binding proteins: Biochemical and electron microscopic evidence for site-specific reassociation between spectrin and bands 2.1 and 4.1. Proc Natl Acad Sci USA 1979;76:5192-6. (Pubitemid 10236105)
7. 327 substitution in the cytoplasmic domain of erythrocyte band 3 protein associated with spherocytoc hemolytic anemia and partial deficiency of protein 4.2. Blood 1992;80:523-9.
8. Korsgren C, Cohen CM. Purification and properties of human erythrocyte band 4.2. J Biol Chem 1986;261:5536-43.
9. Korsgren C, Cohen CM. Associations of human erythrocyte band 4.2. J Biol Chem 1988;263:10212-8.
10. Tshilolo L, Kagambega F, Sztern B, Vertongen F, Gulbis B. La sphérocytose héréditaire: Bilan d'une année d'expérience de l'étude des protéines de la membrane érythrocytaire. Rev Med Brux 1998;5:417-23. (Pubitemid 128519438)
11. Streichman S, Gescheidt Y. Cryohemolysis for the detection of hereditary spherocytosis: Correlation studies with osmotic fragility and autohemolysis. Am J Hematol 1998;58:206-12. (Pubitemid 28303549)
12. Iglauer A, Reinhadt D, Schröter W, Pekrun A. Cryohemolysis test as a diagnostic tool for hereditary spherocytosis. Ann Hematol 1999;78:555-7. (Pubitemid 30009357)
13. King MJ, Behrens J, Rogers C, Flynn C. Rapid flow cytometric test for the diagnosis of membrane cytoskeleton-associated haemolytic anaemia. Br J Haematol 2000;111:924-33. (Pubitemid 32098933)
14. Girodon F, Garçon L, Bergoin E, Largier M, Delaunay J, Fénéant-Thibault M, et al. Usefulness of the eosin-5'-maleimide cytometric method as a first-line screening test for the diagnosis of hereditary spherocytosis: Comparison with ektacytometry and protein electrophoresis. Br J Haematol 2008; 140:468-70.
15. Sahr KE, Laurila P, Kotula L, Scarpa AL, Coupal E, Leto TL, et al. The complete cDNA and polypeptide sequences of human erythroid alpha-spectrin. J Biol Chem 1990;265:4434-43.
16. Winkelmann JC, Chang JG, Tse WT, Scarpa AL, Marchesi VT, Forget BG. Full-length sequence of the cDNA for human erythroid beta-spectrin. J Biol Chem 1990;265:11827-32.
17. Lux SE, John KM, Kopito RR, Lodish HF. Cloning and characterization of band 3, the human erythrocyte anion-exchange protein (AE1). Proc Natl Acad Sci USA 1989;86:9089-93. (Pubitemid 20010370)
18. Sung LA, Chien S, Chang LS, Lambert K, Bliss SA, Bouhassira EE, et al. Molecular cloning of human protein 4.2: A major component of the erythrocyte membrane. Proc Natl Acad Sci USA 1990;87:955-9.
19. Gallagher PG, Forget BG. Hematologically important mutations: Spectrin and ankyrin variants in hereditary spherocytosis. Blood Cells Mol Dis 1998;24:539-43. (Pubitemid 29086936)
20. Zhu K, Nguyen M, Strong W, Whitman-Guliaev C. Performance comparison of the Experion™ automated electrophoresis system and SDS-PAGE for protein analysis. Bio-Rad laboratories Inc.: Tech Note 5299; 2005.
21. Hassoun H, Palek J. Hereditary spherocytosis: A review of the clinical and molecular aspects of the disease. Blood Rev 1996; 10:129-47. (Pubitemid 26383608)
22. Saad ST, Costa FF, Vincentim DL, Salles TS, Pranke PH. Red cell membrane protein abnormalities in hereditary spherocytosis in Brazil. Br J Haematol 1994;88:295-9. (Pubitemid 24318176)