Validation and accreditation of a duplex real-time PCR test for reliable in planta detection of Chalara fraxinea

EPPO Bulletin

Volumn 41, Issue 1, 2011, Pages 21-26

  Ioos, R ^a   Fourrier, C ^a  

^a Station de Mycologie   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CHALARA; FUNGI;

EID: 79952673025     PISSN: 02508052     EISSN: 13652338     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2010.02430.x     Document Type: Article

References (8)

1. Chandelier A, André F & Laurent F (2009) Detection of Chalara fraxinea in common ash (Fraxinus excelsior) using real time PCR. Forest Pathology 40, 87-95.
2. EPPO (2010) PM 7/98 (1): Specific requirements for laboratories preparing accreditation for a plant pest diagnostic activity. EPPO Bulletin 40, 5-22.
3. Husson C, Scala B, Cael O, Frey P, Feau N & Marçais B (2011) Chalara fraxinea is an invasive pathogen in France. European Journal of Plant Pathology (In press).
4. Ioos R, Fourrier C, Iancu G & Gordon TR (2009a) Sensitive detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction using dual-labeled probe chemistry. Phytopathology 99, 582-590.
5. Ioos R, Kowalski T, Husson C & Holdenrieder O (2009b) Rapid in planta detection of Chalara fraxinea by a real-time PCR assay using a dual-labelled probe. European Journal of Plant Pathology 125, 329-335.
6. Johansson SBK, Vasaitis R, Ihrmark K, Barklund P & Stenlid J (2009) Detection of Chalara fraxinea from tissue of Fraxinus excelsior using species-specific ITS primers. Forest Pathology 40, 111-115.
7. Kowalski T (2006) Chalara fraxinea sp. nov. associated with dieback of ash (Fraxinus excelsior) in Poland. Forest Pathology 36, 264-270.
8. Shewart WA (1939) Statistical Method from the Viewpoint of Quality Control. US Department of Agriculture, Washington, DC.